Centre for Advanced Light Microscopy

Cofunding core facility - Ghent Light Microscopy (GLiM)

Exploring the market potential of photoporation as the next generation of intracellular delivery technology for the R&D and cell therapy markets

Doctoral project Aranit Harizaj

Doctoral project Hendrik Deschout

Doctoral project Jasper Jacobs

Doctoral project Jielin Wang

Doctoral project Jing Liu

Doctoral project Katrien Forier

Doctoral project Laurens Raes

Doctoral project Lotte Vermeulen

Doctoral project Thomas Martens

Engineering of universal iPSC derived therapeutic cells by nanofiber photoporation

Genetic modification of cells by photoporation for cancer research and treatment

In-depth analysis of vapor nanobubble-mediated permeabilization at the level of the plasma membrane and nuclear envelope.

Layer-by-layer self-assembly technique: a tunable tool for the delivery of therapeutic nucleic acids.

LBL delivery systems for intracellular ‎delivery ‎of therapeutic nucleic ‎acids.

Non-viral engineering of natural killer cells: exploring nanoparticle-sensitized photoporation as a promising transfection technology.

Nuclear photoporation for enhancing intranuclear delivery and studying laminopathies

Towards cell state-preserving delivery of therapeutic macromolecules into macrophages with polydopamine-sensitized photoporation.

Towards minimally invasive delivery of large molecules into human T cells by photoporation with polydopamine nanosensitizers and photothermal nanofibers.

Unfolding the versatility of laser-induced vapor nanobubbles: from balancing intracellular delivery with regulated cell death to loading of extracellular vesicles.

Design of lipid nanoparticles for RNA-based ex vivo T cell engineering in cancer immunotherapy

Development and polymerization of novel 2-alkyl-2-oxazine monomers towards cationic polymers for gene delivery

Doctoral project Alexander Tishchenko Khoruzh

Doctoral project Emile Drijvers

Doctoral project Ewoud Cosaert

Doctoral project Jolien Vitse

Doctoral project Olivier De Clercq

Doctoral project Rita Sobral Fernandes Machado dos Santos

Doctoral project Robert Jansens

Formation and stability of protein particles with different properties in pharmaceutical formulations and biological fluids

Generation of iPSC-derived CAR-T cells

Generation of iPSC-derived hematopoietic stem cells.

Light-responsive polymer for efficient intracellular delivery of biomacromolecules

Luminescent nanoparticles for in vivo bioimaging

Novel strategies to treat vitreous opacities

Ocular delivery of base editors for genome editing in the retina

Photoporation of fluorescent nanobodies for super resolution imaging and structure-function analysis of immune and cancer cell invadosomes.

Uncovering the mechanism underlying and consequences of alphaherpesvirus-induced formation of tunneling nanotubes (TNTs)

A microfluidics approach towards contact-free laser-induced photoporation for cell-based therapies

A microfluidics approach towards contact-free laser-induced photoporation for cell-based therapies

Benchtop photoporator

BOF staff support in the framework of an ERC-Grant

Centre for nano- and biophotonics

Combining photoporation with electrophoresis for improved transfection of hard-to-transfect cells

Development and evaluation of specific strategies for the targeted labeling of intracellular structures with quantum dots in live cells

Development of advanded fluorescence microscopy methods for studying the structure, the transport and stability of nanomedicines for gene therapy of the retinal pigment epithelium.

Development of nanophosphors for tracking of dendritic cells in immunotherapy

Drug loading and genetic engineering of mesenchymal stem cells through photoporation for the treatment of glioblastoma and osteoarthritis

Engineering of universal iPSC-derived therapeutic cells by nanofiber photoporation.

Engineering of universal iPSC-derived therapeutic cells by nanofiber photoporation.

Exosome staging to predict axillary lymph node metastasis in early breast cancer.

Extracellular vesicle (EV) transport across the brain barriers: from mechanistic and biological insights towards strategies for delivery of therapeutics to the brain.

FB-III (“FB triple-I”) : Flanders BioImaging: Integrating Imaging Infrastructure from cell to man.

Identification of individual exosome like vesicles by surface enhanced Raman spectroscopy

Improved intracellular delivery of upconversion nanoparticles by laser-induced vapor nanobubbles for multimodal bio-imaging applications

Innovation mandate with spin-off: Photoporation for in vitro / ex vivo transfecting of cells

Laser bench for laser scanning confocal microscopy

Laser illumination system for laser-based widefield fluorescence microscopy

Laser-induced vapor nanobubbles and light-responsive nanocarriers for improved treatment of biofilm-related infections

Laser induced vapour nanobubbles as a novel therapeutic approach for treating biofilm-related infections

Laser-induced vapour nanobubbles for intracellular delivery of nanomaterials and treatment of biofilm infections

Light-triggered nanobombs for intracellular delivery of functional macromolecules

Light-triggered self-assembled nanostructures for efficient and safe production of engineered therapeutic immune cells

NOn-VIral gene modified STEM cell therapy

Non-viral gene therapy: overcoming the endosomal barrier

Optical molecular microscopy

Photoporation as platform technology to study intercellular communication by Extracellular Vesicles and to facilitate their therapeutic use

Quantum dot nanoparticles for live cell imaging and intracellular targeting.

Selective photoporation of the nuclear envelope to mimic pathological defects and facilitate intranuclear delivery.

Stimulated emission depletion microscopy (STED): Nanoscopy for innovative research in the life sciences

Unravelling the cellular response to photoporation

Verhaert demonstrator project

Water vapor nano bubbles as a new concept for the delivery of nanoparticles in the cytoplasm of target cells for the inactivation of biofilms

Analysis and prevention of protein particle formation – An approach towards safer antibody drugs in the clinic

Collaboration on the optimisation of macromolecular pharmaceutical access to cellular targets (COMPACT)

Disposable sample holder with integrated optics for enabling Light Sheet Microscopy on standard light microscopes

Evaluation of cellular dynamics using automated widefield imaging

Fluorescence correlation spectroscopy

Light for life: high contrast multimodal medical imaging using inorganic fluorophores

Multitasking nanoparticles for intracellular imaging and biosensing

Penetration of antimicrobial agents into biofilms and use of quorum sensing inhibitors as novel anti-biofilm compounds

Perturbation of apolipoprotein E4 (apoE4) activity in AD pathology using single domain antibodies and targeting them to the brain

Photo-induced destruction of protein aggregates and liquefaction of the vitreous humour using light sensitive nanomaterials.

Photoporation of fluorescent nanobodies for super resolution imaging and structure-function analysis of immune and cancer cell invadosomes.

Photoporation of the inner limiting membrane to enhance retinal drug delivery after intravitreal injection

Quorum sensing and outer membrane vesicles : intra-and interspecies communication of Stenotrophomonas maltophilia 

Single-cell ICP-mass spectrometry in the biological sciences: on the way to real-world use

Tunneling nanotubes induced by the US3 protein of alphaherpesviruses

Ultrafast-scanning tandem ICP-mass spectrometer for interference-free and highly-sensitive transient signal monitoring

Uncovering the mechanism underlying and consequences of alphaherpesvirus-induced formation of tunneling nanotubes (TNTs)

Uncovering the mechanism underlying and consequences of alphaherpesvirus-induced formation of tunneling nanotubes (TNTs)

Upgrade of confocal FLIM microscope with two-photon excitation and detection modules

White light laser-based confocal fluorescence lifetime imaging microscope for multi-parameter 3D live imaging

Biophysical study of the absorption and transport of nanomaterials in living cells in a biopharmaceutical environment.