Project

Fluorescence correlation spectroscopy

Code
01B01108
Duration
01 January 2009 → 31 December 2010
Funding
Regional and community funding: Special Research Fund
Research disciplines
  • Natural sciences
    • Characterisation of biologically active (macro)molecules
    • Analytical biochemistry
    • Cellular interactions and extracellular matrix
    • Plant cell and molecular biology
  • Medical and health sciences
    • Molecular biophysics
    • Other organic elements and compounds
    • Cellular interactions and extracellular matrix
    • Molecular biophysics
    • Other organic elements and compounds
    • Biomarker discovery
    • In vitro testing
    • Biopharmaceutics
    • Phytochemistry
    • Clinical pharmacy
    • Analytical toxicology
    • Molecular biophysics
    • Genetics
  • Engineering and technology
    • Biomaterials
    • Biomedical modelling
    • Biochemical engineering
    • Molecular and cellular biomechanics
    • Environmental biotechnology diagnostics
    • Industrial molecular engineering of nucleic acids and proteins
Keywords
fluorescence microscopy drug delivery nanomaterials
 
Project description

Fluorescence Correlation Spectroscopy (FCS) is an advanced microscopy technique that allows to gather quantitative information on the dynamic behaviour and stability of nanoparticles and molecules. Our research group demonstrated the possibility of FCS to study the stability of macromolecular drugs mainly in vitro (e.g. buffers, vitreous and blood). It is however essential to be able to perform FCS measurements also in living cells. This project evaluates the use of FCS to study the behaviour of nanomaterials in living cells.