Project

Mutational analysis of the glycosylation reactions catalysed by GH-13

Code
3F009812
Looptijd
01-10-2012 → 30-09-2016
Financiering
Regional and community funding: Special Research Fund, Research Foundation - Flanders (FWO)
Onderzoeksdisciplines
  • Engineering and technology
    • Biocatalysis and enzyme technology
    • Bioprocessing, bioproduction and bioproducts
    • Fermentation
    • Industrial microbiology
    • Industrial molecular engineering of nucleic acids and proteins
Trefwoorden
biokatalyse glycosylering enzyme engineering
 
Projectomschrijving

Glycosylation can have a profound effect on the biological and physicochemical properties of a molecule. In this project, glycosylation reactions catalysed by GH-13 enzymes will be analysed by the systematic mutagenesis of aminoacids in their active site. Based on the obtained information, the enzymes will subsequently be optimised through enzyme engineering to increase their activity towards non-carbohydrate acceptors.