Project

Mutational analysis of the glycosylation reactions catalysed by GH-13

Duration
01 October 2012 → 30 September 2016
Funding
Regional and community funding: Special Research Fund, Research Foundation - Flanders (FWO)
Research disciplines
  • Engineering and technology
    • Biocatalysis and enzyme technology
    • Bioprocessing, bioproduction and bioproducts
    • Fermentation
    • Industrial microbiology
    • Industrial molecular engineering of nucleic acids and proteins
Keywords
glycosylation biocatalysis enzyme engineering
 
Project description

Glycosylation can have a profound effect on the biological and physicochemical properties of a molecule. In this project, glycosylation reactions catalysed by GH-13 enzymes will be analysed by the systematic mutagenesis of aminoacids in their active site. Based on the obtained information, the enzymes will subsequently be optimised through enzyme engineering to increase their activity towards non-carbohydrate acceptors.