Project

Bioinformatics for a high-throughput deep mutational scanning protein-protein interaction assay: turning a prototype into a precision medicine toolbox

Code
01D18619
Duration
01 October 2019 → 31 October 2020
Funding
Regional and community funding: Special Research Fund
Research disciplines
  • Natural sciences
    • Bioinformatics of disease
    • Development of bioinformatics software, tools and databases
    • Structural bioinformatics and computational proteomics
Keywords
Protein-protein interactions deep mutational scanning high-throughput screening omics and bioinformatics precision medicine
 
Project description

This proposal contributes to the development of a high-throughput method to characterize the impact of nearly every mutation on specific protein-protein interactions. After bioinformatics-driven experimental optimization, this method will be applied to tumor-driver protein interactions (TP53, PTEN, PIK3R1) and results compared to large-scale tumor mutation and gene expression data. This will allow to demonstrate the major relevance for personalized medicine.