Research Explorer

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Researcher

Stefaan De Smedt

Awards & Distinctions

517 Results

2025

Acoustic tweezers for targeted drug delivery

Léa GuerassimoffStefaan De SmedtFélix SauvageMichael Baudoin

A1 Journal Article in ADVANCED DRUG DELIVERY REVIEWS

Current state of mRNA vaccine development against Mycobacterium tuberculosis

Ilke AernoutRein VerbekeStefaan De SmedtFrancis ImpensIne Lentacker

Bookchapter in Trends in mRNA vaccine research

Exploring the interface : the interaction between pulmonary surfactant and RNA therapeutics

Lore HermanKoen RaemdonckStefaan De Smedt

Impact of pneumatic tube transportation on the aggregation of monoclonal antibodies in clinical practice

Michaela CohrsNele ClottensPieter RamautKevin BraeckmansStefaan De SmedtTieneke BautersHristo Svilenov

A1 Journal Article in EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES

Intrinsic differential scanning fluorimetry for protein stability assessment in microwell plates

Michaela CohrsAlastair DavyManon Van AckereStefaan De SmedtKevin BraeckmansMarkus EpeHristo Svilenov

A1 Journal Article in MOLECULAR PHARMACEUTICS

Surfactant protein B-derived peptides as endosomal escape enhancers for pulmonary delivery of siRNA

Lore HermanRoberta GuagliardoAgata ZamborlinQiaoyu LiuJesús Pérez-GilStefaan De SmedtKoen Raemdonck

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

2024

Alpha-galactosylceramide improves the potency of mRNA LNP vaccines against cancer and intracellular bacteria

Sofie MeulewaeterIlke AernoutJoke DeprezYanou EngelenMargo De VelderLorenzo FranceschiniKarine BreckpotSerge Van CalenberghCaroline AsselmanKatie Boucher et al.

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Beyond chloroquine : cationic amphiphilic drugs as endosomal escape enhancers for nucleic acid therapeutics

Aliona DebisschopBram BogaertCristina MunteanStefaan De SmedtKoen Raemdonck

A1 Journal Article in CURRENT OPINION IN CHEMICAL BIOLOGY

Considerations on the design of lipid-based mRNA vaccines against cancer

Sofie MeulewaeterYao ZhangAbishek WadhwaKevin FoxIne LentackerKenneth W. HarderPieter R. CullisStefaan De SmedtMiffy H.Y. ChengRein Verbeke

A1 Journal Article in JOURNAL OF MOLECULAR BIOLOGY

Evaluating β2-agonists as siRNA delivery adjuvants for pulmonary surfactant-coated nanogel inhalation therapy

Pieterjan MerckxGRIET CONICKXEvy BlommeTania MaesKen BrackeGuy BrusselleStefaan De SmedtKoen Raemdonck

A1 Journal Article in EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS

Immunopeptidomics mapping of Listeria monocytogenes T cell epitopes in mice

Adillah GulLecia L. PewePatrick WillemsRupert MayerFabien Henri TheryCaroline AsselmanIlke AernoutRein VerbekeDenzel EggermontLaura Van Moortel et al.

A1 Journal Article in MOLECULAR & CELLULAR PROTEOMICS

Laser-induced vapor nanobubbles for B16-F10 melanoma cell killing and intracellular delivery of chemotherapeutics

Jana RamonYanou EngelenHerlinde De KeersmaeckerIlia GoemaereDeep PunjJulián Mejía MoralesCédric BonteGeert BerxEsther HosteStephan Stremersch et al.

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Less is more : self-amplifying mRNA becomes self-killing upon dose escalation in immune-competent retinal cells

Helena VanlucheneOriane GillonKaren PeynshaertStefaan De SmedtNiek SandersKoen RaemdonckKatrien Remaut

A1 Journal Article in EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS

Nanomedicine to aid immunogenic cell death (ICD)-based anticancer therapy

Robin DemuynckYanou EngelenAndre SkirtachStefaan De SmedtIne LentackerDmitri Krysko

A1 Journal Article in TRENDS IN CANCER

Non-viral delivery of RNA for therapeutic T cell engineering

Dominika BerdeckaStefaan De SmedtWinnok H. De VosKevin Braeckmans

A1 Journal Article in ADVANCED DRUG DELIVERY REVEIWS

Nucleic acid degradation as barrier to gene delivery : a guide to understand and overcome nuclease activity

Heyang ZhangJo VandesompeleKevin BraeckmansStefaan De SmedtKatrien Remaut

A1 Journal Article in CHEMICAL SOCIETY REVIEWS

Optimizing high-intensity focused ultrasound-induced immunogenic cell-death using passive cavitation mapping as a monitoring tool

Yanou EngelenDmitri KryskoIuliia EfimovaKarine BreckpotMichel VersluisStefaan De SmedtGuillaume LajoinieIne Lentacker

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Photoporation of NK-92MI cells with biodegradable polydopamine nanosensitizers as a promising strategy for the generation of engineered NK cell therapies

Charlotte HinnekensAranit HarizajDominika BerdeckaIlke AernoutMolood ShariatiStefanie PeetersEva LionStefaan De SmedtBart VandekerckhoveKevin Braeckmans et al.

A1 Journal Article in APPLIED MATERIALS TODAY

Selective replacement of cholesterol with cationic amphiphilic drugs enables the design of lipid nanoparticles with improved RNA delivery

Bram BogaertAliona DebisschopThomas EhouarneHannelore Van EeckhoutteJoyceline De VolderAn JacobsEline PottieRiet De RyckeAurélie CrabbéPieter Mestdagh et al.

A1 Journal Article in NANO LETTERS

Supramolecular dye nanoassemblies for advanced diagnostics and therapies

Pouria RamezaniStefaan De SmedtFélix Sauvage

A1 Journal Article in BIOENGINEERING & TRANSLATIONAL MEDICINE

Towards the clinical evaluation of Galsome-NEO, a glycolipid-adjuvanted mRNA cancer vaccine

Sofie MeulewaeterStefaan De SmedtIne Lentacker

2023

A lipid nanoparticle platform for RNA delivery with cationic amphiphilic drugs : merging the best of both worlds

Bram BogaertKoen RaemdonckStefaan De Smedt

CO2-driven nebulization of pH-sensitive supramolecular polymers for intraperitoneal hydrogel formation and the treatment of peritoneal metastasis

Helena BraetPeter-Paul FransenRemco MariënGiovanna LolloWim CeelenChris VervaetLieve BalcaenFrank VanhaeckeChristian VanhoveStefan van der Vegte et al.

A1 Journal Article in ACS APPLIED MATERIALS & INTERFACES

Continuous freeze-drying of messenger RNA lipid nanoparticles enables storage at higher temperatures

Sofie MeulewaeterGust NuyttenMiffy H.Y. ChengStefaan De SmedtPieter R. CullisThomas De BeerIne LentackerRein Verbeke

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Delivery of macromolecules in unstimulated T cells by photoporation with polydopamine nanoparticles

Dominika BerdeckaAranit HarizajIlia GoemaereDeep PunjGlenn GoetgelukStijn De MunterHerlinde De KeersmaeckerVeerle BoterbergPeter DubruelBart Vandekerckhove et al.

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Designing of gradient scaffolds and their applications in tissue regeneration

Ananya PattnaikA. Swaroop SanketSanghamitra PradhanRajashree SahooSudiptee DasSwarnaprbha PanyTimothy E. L. DouglasRambabu DandelaQiang LiuJaykumar Rajadas et al.

A1 Journal Article in BIOMATERIALS

Every nano-step counts : a critical reflection on do’s and don’ts in researching nanomedicines for retinal gene therapy

Karen PeynshaertJoke DevoldereStefaan De SmedtKatrien Remaut

A1 Journal Article in EXPERT OPINION ON DRUG DELIVERY

High pressure nebulization of smart hydrogels : driving intraperitoneal drug delivery forward

Helena BraetKatrien RemautStefaan De Smedt

Immunotherapy : towards engineered NK cell therapy : mRNA transfection of NK92 cells by photoporation with biodegradable polydopamine nanosensitizersHOTOPORATION WITH BIODEGRADABLE POLYDOPAMINE NANOSENSITIZERS

Charlotte HinnekensAranit HarizajIlia GoemaereStefaan De SmedtBart VandekerckhoveJuan FraireKevin Braeckmans

In vitro and ex vivo models for assessing drug permeation across the cornea

Inès De HoonRabah BoukherroubStefaan De SmedtSabine SzuneritsFélix Sauvage

A1 Journal Article in MOLECULAR PHARMACEUTICS

Influence of the size and charge of carbon quantum dots on their corneal penetration and permeation enhancing properties

Inès De HoonAlexandre BarrasTomasz SwebockiBernd VanmeerhaegheBram BogaertCristina MunteanAmar AbderrahmaniRabah BoukherroubStefaan De SmedtFélix Sauvage et al.

A1 Journal Article in ACS APPLIED MATERIALS & INTERFACES

Light-triggered mechanical disruption of extracellular barriers by swarms of enzyme-powered nanomotors for enhanced delivery

Juan FraireMaria GuixAna C. HortelaoNoelia Ruiz-GonzálezAnna C. BakeneckerPouria RamezaniCharlotte HinnekensFélix SauvageStefaan De SmedtKevin Braeckmans et al.

A1 Journal Article in ACS NANO

Nanobody-mediated SPECT/CT imaging reveals the spatiotemporal expression of programmed death-ligand 1 in response to a CD8+ T cell and iNKT cell activating mRNA vaccine

Thomas ErtveldtSofie MeulewaeterYannick De VlaeminckOscar OlarteKatrijn BroosSerge Van CalenberghStephanie BourgeoisJoke DeprezYves HeremansCleo Goyvaerts et al.

A1 Journal Article in THERANOSTICS

Non-viral engineering of NK cells

Charlotte HinnekensStefaan De SmedtJuan FraireKevin Braeckmans

A1 Journal Article in BIOTECHNOLOGY ADVANCES

Photothermal nanofibers enable macromolecule delivery in unstimulated human T cells

Dominika BerdeckaManon MinsartTao LuDeep PunjRiet De RyckeMina NikolićEduardo Bolea FernandezFrank VanhaeckeRanhua XiongStefaan De Smedt et al.

A1 Journal Article in APPLIED MATERIALS TODAY

Photothermal nanomaterial-mediated photoporation

Ranhua XiongFélix SauvageJuan FraireChaobo HuangStefaan De SmedtKevin Braeckmans

A1 Journal Article in ACCOUNTS OF CHEMICAL RESEARCH

Principles of antibodies with ultralong complementarity-determining regions and picobodies

Marcel Fridolin PassonStefaan De SmedtHristo Svilenov

A1 Journal Article in BIOTECHNOLOGY ADVANCES

Repositioning the antihistamine ebastine as an intracellular siRNA delivery enhancer

Cristina MunteanEva BlondeelLaure HarinckKunal PednekarJai PrakashOlivier De WeverJeanne Leblond ChainStefaan De SmedtKatrien RemautKoen Raemdonck

A1 Journal Article in INTERNATIONAL JOURNAL OF PHARMACEUTICS

Response surface methodology to efficiently optimize intracellular delivery by photoporation

Ilia GoemaereDeep PunjAranit HarizajJessica WoolstonSofie ThysKaren SterckStefaan De SmedtWinnok H. De VosKevin Braeckmans

A1 Journal Article in INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Smart hydrogels delivered by high pressure aerosolization can prevent peritoneal adhesions

Helena BraetPeter-Paul FransenYong ChenSimon Van HerckRemco MariënValérie VanhoorneWim CeelenAnnemieke MadderSteven BalletRichard Hoogenboom et al.

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

The alpha-adrenergic antagonist prazosin promotes cytosolic siRNA delivery from lysosomal compartments

Thijs Van de VyverCristina MunteanIuliia EfimovaDmitri KryskoLynn De BackerStefaan De SmedtKoen Raemdonck

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

The effect of electrostatic high pressure nebulization on the stability, activity and ex vivo distribution of ionic self-assembled nanomedicines

Helena BraetValentina AndrettoRemco MariënBeyza YücesanStefan van der VegteRagna HaegebaertGiovanna LolloStefaan De SmedtKatrien Remaut

A1 Journal Article in ACTA BIOMATERIALIA

Transport by circulating myeloid cells drives liposomal accumulation in inflamed synovium

Joke DeprezRein VerbekeSofie MeulewaeterIlke AernoutHeleen DewitteTine DecruyJulie CoudenysJulie Van DuyseGert Van IsterdaelDan Peer et al.

A1 Journal Article in NATURE NANOTECHNOLOGY

2022

A lipid nanoparticle platform for mRNA delivery through repurposing of cationic amphiphilic drugs

Bram BogaertFelix SauvageRoberta GuagliardoCristina Muntean[missing] Van Phuc NguyenEline PottieMike WelsAn-Katrien MinnaertRiet De RyckeQiangbing Yang et al.

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Bio-nanohybrid gelatin/quantum dots for cellular imaging and biosensing applications

Sangram Keshari SamalStefaan SoenenDario PuppiKarolien De WaelSanghamitra PatiStefaan De SmedtKevin BraeckmansPeter Dubruel

A1 Journal Article in INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Evaluation of liposome-loaded microbubbles as a theranostic tool in a murine collagen-induced arthritis model

Joke DeprezSilke RooversGuillaume LajoinieHeleen DewitteTine DecruyJulie CoudenysBenedicte DescampsChristian VanhoveMichel VersluisDirk Elewaut et al.

A2 Journal Article in SCIENTIA PHARMACEUTICA

Exploring biochemical and biophysical methods for the delivery of biologics to the corneal epithelium

Mike WelsFélix SauvageStefaan De Smedt

Fluorine MR imaging probes dynamic migratory profiles of perfluorocarbon-loaded dendritic cells after streptozotocin-induced inflammation

Shweta SainiAn-Sofie VanherwegenSayuan LiangRein VerbekeHannelie KorfIne LentackerStefaan De SmedtConny GysemansUwe Himmelreich

A1 Journal Article in MOLECULAR IMAGING AND BIOLOGY

Gas-shearing synthesis of core–shell multicompartmental microparticles as cell-like system for enzymatic cascade reaction

Qingli QuXiaoli ZhangHossein RavanbakhshGuosheng TangJian ZhangYankang DengKevin BraeckmansStefaan De SmedtRanhua XiongChaobo Huang

A1 Journal Article in CHEMICAL ENGINEERING JOURNAL

Gene therapy to enhance angiogenesis in chronic wounds

Elnaz Shaabani SichaniMaryam SharifiaghdamReza Faridi-MajidiStefaan De SmedtKevin BraeckmansJuan Fraire

A1 Journal Article in MOLECULAR THERAPY-NUCLEIC ACIDS

ICG-mediated photodisruption of the inner limiting membrane enhances retinal drug delivery

Karen PeynshaertHelena VanlucheneKaat De ClerckAn-Katrien MinnaertMorgane VerhoevenNoémie GouspillouNezahat BostanToshio HisatomiGeraldine AccouFélix Sauvage et al.

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Immunopeptidomics-based design of mRNA vaccine formulations against Listeria monocytogenes

Rupert MayerRein VerbekeCaroline AsselmanIlke AernoutAdillah GulDenzel EggermontKatie BoucherFabien Henri TheryTeresa MaiaHans Demol et al.

A1 Journal Article in NATURE COMMUNICATIONS

Laser-induced nanobubbles safely ablate vitreous opacities in vivo

Félix SauvageVan Phuc NguyenYanxiu LiAranit HarizajJ. SebagDimitri RoelsViktor Van HavereKaren PeynshaertRanhua XiongJuan Fraire et al.

A1 Journal Article in NATURE NANOTECHNOLOGY

Light triggered nanoscale biolistics for efficient intracellular delivery of functional macromolecules in mammalian cells

Juan FraireElnaz Shaabani SichaniMaryam SharifiaghdamMatthias RombautCharlotte HinnekensDawei HuaJana RamonLaurens RaesEduardo Bolea FernandezToon Brans et al.

A1 Journal Article in NATURE COMMUNICATIONS

Modulating intracellular pathways to improve non-viral delivery of RNA therapeutics

Thijs Van de VyverStefaan De SmedtKoen Raemdonck

A1 Journal Article in ADVANCED DRUG DELIVERY REVIEWS

Multicompartmental microcapsules for enzymatic cascade reactions prepared through gas shearing and surface gelation

Qingli QuWeixia ChengXiaoli ZhangAying ZhouYankang DengMiaomiao ZhuTianjiao ChuBella B. ManshianRanhua XiongStefaan J. Soenen et al.

A1 Journal Article in BIOMACROMOLECULES

Non-invasive cell-tracking methods for adoptive T cell therapies

Jelter Van HoeckChristian VanhoveStefaan De SmedtKoen Raemdonck

A1 Journal Article in DRUG DISCOVERY TODAY

Non-viral siRNA delivery to T cells : challenges and opportunities in cancer immunotherapy

Jelter Van HoeckKevin BraeckmansStefaan De SmedtKoen Raemdonck

A1 Journal Article in BIOMATERIALS

Ocular enzyme delivery and nanotechnology : expanding the therapeutic horizon for vitreoretinal diseases

An-Katrien MinnaertKatrien RemautStefaan De Smedt

Photodisruption of the inner limiting membrane : exploring ICG loaded nanoparticles as photosensitizers

Kaat De ClerckGeraldine AccouFélix SauvageKevin BraeckmansStefaan De SmedtKatrien RemautKaren Peynshaert

A1 Journal Article in PHARMACEUTICS

The cellular response to plasma membrane disruption for nanomaterial delivery

Gaëlle HouthaeveStefaan De SmedtKevin BraeckmansWinnok H. De Vos

A1 Journal Article in NANO CONVERGENCE

Transient nuclear lamin A/C accretion aids in recovery from vapor nanobubble-induced permeabilisation of the plasma membrane

Gaëlle HouthaeveGerardo García-Díaz BarrigaStephan StremerschHerlinde De KeersmaeckerJuan FraireJo VandesompelePieter MestdaghStefaan De SmedtKevin BraeckmansWinnok De Vos

A1 Journal Article in CELLULAR AND MOLECULAR LIFE SCIENCES

Yeast-produced fructosamine-3-kinase retains mobility after ex vivo intravitreal injection in human and bovine eyes as determined by Fluorescence Correlation Spectroscopy

An-Katrien MinnaertLoes van SchieHendrik GrootaertJonas HimpeSimon DevosWannes WeytsHerlinde De KeersmaeckerKevin BraeckmansElisabeth Van AkenJoris Delanghe et al.

A1 Journal Article in INTERNATIONAL JOURNAL OF PHARMACEUTICS

2021

Black phosphorus mediated photoporation : a broad absorption nanoplatform for intracellular delivery of macromolecules

Jielin WangAranit HarizajYongbo WuXiaofang JiangToon BransJuan FraireJulián Mejía MoralesStefaan De SmedtZhilie TangRanhua Xiong et al.

A1 Journal Article in NANOSCALE

Bubble forming films for spatial selective cell killing

Dawei HuaAranit HarizajMike WelsToon BransStephan StremerschHerlinde De KeersmaeckerEduardo Bolea FernandezFrank VanhaeckeDimitri RoelsKevin Braeckmans et al.

A1 Journal Article in ADVANCED MATERIALS

Carbon quantum dots as a dual platform for the inhibition and light-based destruction of collagen fibers : implications for the treatment of eye floaters

Alexandre BarrasFélix SauvageInès De HoonKevin BraeckmansDawei HuaGaëtan BuvatJuan FraireChristophe LethienJ. SebagMichael Harrington et al.

A1 Journal Article in NANOSCALE HORIZONS

Cas9 RNP transfection by vapor nanobubble photoporation for ex vivo cell engineering

Laurens RaesMelissa PilleAranit HarizajGlenn GoetgelukJelter Van HoeckStephan StremerschJuan FraireToon BransOlivier Gerrit de JongRoel Maas-Bakker et al.

A1 Journal Article in MOLECULAR THERAPY-NUCLEIC ACIDS

Challenges and strategies for the delivery of biologics to the cornea

Mike WelsD. RoelsKoen RaemdonckStefaan De SmedtFélix Sauvage

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Concentration gradients in material sciences : methods to design and biomedical applications

Dawei HuaRanhua XiongKevin BraeckmansBenoit ScheidChaobo HuangFélix SauvageStefaan De Smedt

A1 Journal Article in ADVANCED FUNCTIONAL MATERIALS

Cytosolic delivery of gadolinium via photoporation enables improved in vivo magnetic resonance imaging of cancer cells

Aranit HarizajBenedicte DescampsChristophe MangodtStephan StremerschArianna StoppaLieve BalcaenToon BransHilde De RoosterNausikaa DevriendtJuan Fraire et al.

A1 Journal Article in BIOMATERIALS SCIENCE

Delivering on the promise of adoptive T cell therapy : assessment of non-viral methods for direct cytosolic delivery of membrane-impermeable cargo

Jelter Van HoeckKoen RaemdonckStefaan De Smedt

Enhanced siRNA delivery and selective apoptosis induction in H1299 cancer cells by layer-by-layer-assembled Se nanocomplexes : toward more efficient cancer therapy

Maryam SharifiaghdamElnaz Shaabani SichaniZeynab SharifiaghdamHerlinde De KeersmaeckerRiet De RyckeStefaan De SmedtReza Faridi-MajidiKevin BraeckmansJuan Fraire

A1 Journal Article in FRONTIERS IN MOLECULAR BIOSCIENCES

Exploring (bio-)polymeric matrices and light for advanced pharmaceutical and medical applications

Dawei HuaStefaan De SmedtChaobo HuangRanhua XiongFélix Sauvage

Exploring high pressure nebulization of pluronic F127 hydrogels for intraperitoneal drug delivery

Helena BraetMohammad Rahimi GorjiCharlotte DebbautG GhorbaniaslThibault Van WalleghemSenne CornelisSarah CosynsChris VervaetWouter WillaertWim Ceelen et al.

A1 Journal Article in EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS

Exploring novel drug delivery strategies in rheumatoid arthritis : nanomedicines and ultrasound triggered drug delivery

Joke DeprezStefaan De SmedtPeggy JacquesIne Lentacker

Hydrogel-induced cell membrane disruptions enable direct cytosolic delivery of membrane-impermeable cargo

Jelter Van HoeckThijs Van de VyverAranit HarizajGlenn GoetgelukPieterjan MerckxJing LiuMike WelsFélix SauvageHerlinde De KeersmaeckerChristian Vanhove et al.

A1 Journal Article in ADVANCED MATERIALS

Increasing angiogenesis factors in hypoxic diabetic wound conditions by siRNA delivery : additive effect of LbL-gold nanocarriers and desloratadine-induced lysosomal escape

Elnaz Shaabani SichaniMaryam SharifiaghdamJoris LammensHerlinde De KeersmaeckerChris VervaetThomas De BeerElahe MotevaseliMohammad Hossein GhahremaniParvin MansouriStefaan De Smedt et al.

A1 Journal Article in INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Layer by layer assembled chitosan-coated gold nanoparticles for enhanced siRNA delivery and silencing

Elnaz Shaabani SichaniMaryam SharifiaghdamHerlinde De KeersmaeckerRiet De RyckeStefaan De SmedtReza Faridi-MajidiKevin BraeckmansJuan Fraire

A1 Journal Article in INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Lipoplexes to deliver oligonucleotides in gram-positive and gram-negative bacteria : towards treatment of blood infections

Sara PereiraRita Sobral SantosLuís MoreiraNuno GuimarãesMariana GomesHeyang ZhangKatrien RemautKevin BraeckmansStefaan De SmedtNuno Filipe Azevedo

A1 Journal Article in PHARMACEUTICS

Macrophage reprogramming into a pro-healing phenotype by siRNA delivered with LBL assembled nanocomplexes for wound healing applications

Maryam SharifiaghdamElnaz Shaabani SichaniZeynab SharifiaghdamHerlinde De KeersmaeckerBart LucasJoris LammensHossein GhanbariLadan Teimoori-ToolabiChris VervaetThomas De Beer et al.

A1 Journal Article in NANOSCALE

Nanoparticle-sensitized photoporation enables inflammasome activation studies in targeted single cells dagger

Aranit HarizajFilip Van HauwermeirenStephan StremerschRiet De RyckeHerlinde De KeersmaeckerToon BransJuan FraireKarolien GrauwenStefaan De SmedtIne Lentacker et al.

A1 Journal Article in NANOSCALE

Non-viral transfection technologies for next-generation therapeutic T cell engineering

Laurens RaesStefaan De SmedtKoen RaemdonckKevin Braeckmans

A1 Journal Article in BIOTECHNOLOGY ADVANCES

Old drugs with new tricks : repurposing small molecules to promote lysosomal escape of small nucleic acid therapeutics

Thijs Van de VyverKoen RaemdonckStefaan De Smedt

Opening doors with ultrasound and microbubbles : beating biological barriers to promote drug delivery

Joke DeprezG. LajoinieYanou EngelenStefaan De SmedtIne Lentacker

A1 Journal Article in ADVANCED DRUG DELIVERY REVIEWS

PEGylation of recombinant human deoxyribonuclease I decreases its transport across lung epithelial cells and uptake by macrophages

Sohaib MahriEléonore HardyTobias WilmsHerlinde De KeersmaeckerKevin BraeckmansStefaan De SmedtCynthia BosquillonRita Vanbever

A1 Journal Article in INTERNATIONAL JOURNAL OF PHARMACEUTICS

Photoporation with biodegradable polydopamine nanosensitizers enables safe and efficient delivery of mRNA in human T cells

Aranit HarizajMike WelsLaurens RaesStephan StremerschGlenn GoetgelukToon BransBart VandekerckhoveFélix SauvageStefaan De SmedtIne Lentacker et al.

A1 Journal Article in ADVANCED FUNCTIONAL MATERIALS

Photothermal nanofibres enable safe engineering of therapeutic cells

Ranhua XiongDawei HuaJelter Van HoeckDominika BerdeckaLaurens LégerStijn De MunterJuan FraireLaurens RaesAranit HarizajFélix Sauvage et al.

A1 Journal Article in NATURE NANOTECHNOLOGY

Physical transfection technologies for macrophages and dendritic cells in immunotherapy

Aranit HarizajStefaan De SmedtIne LentackerKevin Braeckmans

A1 Journal Article in EXPERT OPINION ON DRUG DELIVERY

Pulmonary surfactant as a versatile biomaterial to fight COVID-19

Lore HermanStefaan De SmedtKoen Raemdonck

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Stimuli-responsive nanobubbles for biomedical applications

Ranhua XiongRonald X. XuChaobo HuangStefaan De SmedtKevin Braeckmans

A1 Journal Article in CHEMICAL SOCIETY REVIEWS

Strategies for controlling the innate immune activity of conventional and self-amplifying mRNA therapeutics : getting the message across

An-Katrien MinnaertHelena VanlucheneRein VerbekeIne LentackerStefaan De SmedtKoen RaemdonckNiek SandersKatrien Remaut

A1 Journal Article in ADVANCED DRUG DELIVERY REVIEWS

Surfactant protein B promotes cytosolic SiRNA delivery by adopting a virus-like mechanism of action

Roberta GuagliardoLore HermanJelle PendersAgata ZamborlinHerlinde De KeersmaeckerThijs Van de VyverSandrine VerstraetenPieterjan MerckxMarie-Paule Mingeot-LeclercqMercedes Echaide et al.

A1 Journal Article in ACS NANO

The dawn of mRNA vaccines : the COVID-19 case

Rein VerbekeIne LentackerStefaan De SmedtHeleen Dewitte

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Together is better : mRNA co‐encapsulation in lipoplexes is required to obtain ratiometric co‐delivery and protein expression on the single cell level

Heyang ZhangJeroen BussmannFlorian H. HuhnkeJoke DevoldereAn-Katrien MinnaertWim JiskootFriedhelm SerwaneJoachim SpatzMagnus RödingStefaan De Smedt et al.

A1 Journal Article in ADVANCED SCIENCE

Vaccinia virus protein B18R : influence on mRNA immunogenicity and translation upon non-viral delivery in different ocular cell types

An-Katrien MinnaertJoke DevoldereKaren PeynshaertLaure VercruysseStefaan De SmedtKatrien Remaut

A1 Journal Article in PHARMACEUTICS

Vapor nanobubble-mediated photoporation constitutes a versatile intracellular delivery technology

Jana RamonRanhua XiongStefaan De SmedtKoen RaemdonckKevin Braeckmans

A1 Journal Article in CURRENT OPINION IN COLLOID & INTERFACE SCIENCE

2020

Beyond the surface : unraveling the intracellular mechanism behind surfactant protein B (SP-B) mediated siRNA delivery

Roberta GuagliardoKoen RaemdonckStefaan De Smedt

Cationic amphiphilic drugs boost the lysosomal escape of small nucleic acid therapeutics in a nanocarrier-dependent manner

Thijs Van de VyverBram BogaertLynn De BackerFreya JorisRoberta GuagliardoJelter Van HoeckPieterjan MerckxSerge Van CalenberghSrinivas RamishettiDan Peer et al.

A1 Journal Article in ACS NANO

Exploring the interface between nanomedicines and aerosolization for intraperitoneal drug delivery

Molood ShariatiKatrien RemautStefaan De SmedtWim Ceelen

Faithful fabrication of biocompatible multicompartmental memomicrospheres for digitally color‐tunable barcoding

Guosheng TangLong ChenZixuan WangShuting GaoQingli QuRanhua XiongKevin BraeckmansStefaan De SmedtYu Shrike ZhangChaobo Huang

A1 Journal Article in SMALL

Fluorescence-based quantification of messenger RNA and plasmid DNA decay kinetics in extracellular biological fluids and cell extracts

Heyang ZhangKoen RomboutsLaurens RaesRanhua XiongStefaan De SmedtKevin BraeckmansKatrien Remaut

A1 Journal Article in ADVANCED BIOSYSTEMS

Influence of pathogenic stimuli on Müller cell transfection by lipoplexes

Karen PeynshaertJoke DevoldereFrederik PhilipsFauve VergauweStefaan De SmedtKatrien Remaut

A1 Journal Article in EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS

Intracellular delivery of mRNA in adherent and suspension cells by vapor nanobubble photoporation

Laurens RaesStephan StremerschJuan FraireToon BransGlenn GoetgelukStijn De MunterLien Van HoeckeRein VerbekeJelter Van HoeckRanhua Xiong et al.

A1 Journal Article in NANO-MICRO LETTERS

Intracellular labeling with extrinsic probes : delivery strategies and applications

Jing LiuJuan FraireStefaan De SmedtRanhua XiongKevin Braeckmans

A1 Journal Article in SMALL

Long-term live-cell microscopy with labeled nanobodies delivered by laser-induced photoporation

Jing LiuTim HebbrechtToon BransEef ParthoensSaskia LippensChengnan LiHerlinde De KeersmaeckerWinnok H. De VosStefaan De SmedtRabah Boukherroub et al.

A1 Journal Article in NANO RESEARCH

Lung surfactant-hydrogel nanocomposites for pulmonary siRNA delivery : breaching barriers for nanomedicine-based inhalation therapy

Pieterjan MerckxKoen RaemdonckStefaan De Smedt

Lyophilization and nebulization of pulmonary surfactant-coated nanogels for siRNA inhalation therapy

Pieterjan MerckxJoris LammensGust NuyttenBram BogaertRoberta GuagliardoTania MaesChris VervaetThomas De BeerStefaan De SmedtKoen Raemdonck

A1 Journal Article in EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS

Materials and technologies to combat counterfeiting of pharmaceuticals : current and future problem tackling

Heyang ZhangDawei HuaChaobo HuangSangram Keshari SamalRanhua XiongFélix SauvageKevin BraeckmansKatrien RemautStefaan De Smedt

A1 Journal Article in ADVANCED MATERIALS

Nanomaterials to avoid and destroy protein aggregates

Félix SauvageJoost SchymkowitzFrederic RousseauBela Z. SchmidtKatrien RemautKevin BraeckmansStefaan De Smedt

A1 Journal Article in NANO TODAY

Nebulization of thermosensitive hydrogels to treat peritoneal carcinomatosis

Helena BraetMohammad Rahimi GorjiCharlotte DebbautWim CeelenStefaan De SmedtKatrien Remaut

Ocular barriers to retinal delivery of intravitreal liposomes : impact of vitreoretinal interface

Shirin TavakoliKaren PeynshaertTatu LajunenJoke DevoldereEva M. del AmoMarika RuponenStefaan De SmedtKatrien RemautArto Urtti

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Surface functionalization with polyethylene glycol and polyethyleneimine improves the performance of graphene-based materials for safe and efficient intracellular delivery by laser-induced photoporation

Jing LiuChengnan LiToon BransAranit HarizajShana Van de SteeneThomas De BeerStefaan De SmedtSabine SzuneritsRabah BoukherroubRanhua Xiong et al.

A1 Journal Article in INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Synergy between intraperitoneal aerosolization (PIPAC) and cancer nanomedicine : cisplatin-loaded polyarginine-hyaluronic acid nanocarriers efficiently eradicate peritoneal metastasis of advanced human ovarian cancer

Molood ShariatiGiovanna LolloKevin MathaBenedicte DescampsChristian VanhoveLeen Van de SandeWouter WillaertLieve BalcaenFrank VanhaeckeJean-Pierre Benoit et al.

A1 Journal Article in ACS APPLIED MATERIALS & INTERFACES

Targeted nanoparticles towards increased L cell stimulation as a strategy to improve oral peptide delivery in incretin-based diabetes treatment

Yining XuHerlinde De KeersmaeckerKevin BraeckmansStefaan De SmedtPatrice D. CaniVéronique PréatAna Beloqui

A1 Journal Article in BIOMATERIALS

The versatility of nucleic acids : a detailed view on mRNA therapeutics and the exploration of oligonucleotides for in-drug labeling

Heyang ZhangKatrien RemautStefaan De Smedt

Vapor nanobubble is the more reliable photothermal mechanism for inducing endosomal escape of siRNA without disturbing cell homeostasis

Juan FraireGaëlle HouthaeveJing LiuLaurens RaesLotte VermeulenStephan StremerschToon BransGerardo Garcia-Diaz BarrigaSarah De KeulenaerFilip Van Nieuwerburgh et al.

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

2019

Aerosolization of nanotherapeutics as a newly emerging treatment regimen for peritoneal carcinomatosis

Molood ShariatiWouter WillaertWim CeelenStefaan De SmedtKatrien Remaut

A1 Journal Article in CANCERS

Biocompatible lipid‐coated persistent luminescent nanoparticles for in vivo imaging of dendritic cell migration

Aranit HarizajOlivier De ClercqBenedicte DescampsChristian VanhoveStefaan De SmedtDirk PoelmanIne LentackerKevin Braeckmans

A1 Journal Article in PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION

Broadening the message : a nanovaccine co-loaded with messenger RNA and α-GalCer induces antitumor immunity through conventional and natural killer T cells

Rein VerbekeIne LentackerKarine BreckpotJonas JanssensSerge Van CalenberghStefaan De SmedtHeleen Dewitte

A1 Journal Article in ACS NANO

Challenges for labeling and longitudinal tracking of adoptively transferred autoreactive T lymphocytes in an experimental type-1 diabetes model

Shweta SainiHannelie KorfSayuan LiangRein VerbekeBella ManshianKoen RaemdonckIne LentackerConny GysemansStefaan De SmedtUwe Himmelreich

A1 Journal Article in MAGNETIC RESONANCE MATERIALS IN PHYSICS BIOLOGY AND MEDICINE

Comparison of MRI properties between multimeric DOTAGA and DO3A gadolinium-dendron conjugates

Maleotane NdiayeVolodymyr MalytskyiThomas VangijzegemFélix SauvageMike WelsCyril CadiouJuliette MoreauCéline HenoumontSébastien BoutryRobert N. Muller et al.

A1 Journal Article in INORGANIC CHEMISTRY

Design of messenger RNA lipid nanoparticles for cancer immunotherapy

Rein VerbekeStefaan De SmedtHeleen Dewitte

Ecofriendly electrospun membranes loaded with visible-light-responding nanoparticles for multifunctional usages : highly efficient air filtration, dye scavenging, and bactericidal activity

Dan LvRuoxue WangGuosheng TangZhipeng MouJiandu LeiJingquan HanStefaan De SmedtRanhua XiongChaobo Huang

A1 Journal Article in ACS APPLIED MATERIALS & INTERFACES

Enhancing nucleic acid delivery with ultrasound and microbubbles

Heleen DewitteSilke RooversStefaan De SmedtIne Lentacker

Bookchapter in Nanotechnology for nucleic acid delivery : methods and protocols

Exploring light-sensitive nanocarriers for simultaneous triggered antibiotic release and disruption of biofilms upon generation of laser-induced vapor nanobubbles

Eline TeirlinckAlexandre BarrasJing LiuJuan FraireTatu LajunenRanhua XiongKatrien ForierChengnan LiArto UrttiRabah Boukherroub et al.

A1 Journal Article in PHARMACEUTICS

Gas‐shearing fabrication of multicompartmental microspheres : a one‐step and oil‐free approach

Guosheng TangRanhua XiongDan LvRonald X. XuKevin BraeckmansChaobo HuangStefaan De Smedt

A1 Journal Article in ADVANCED SCIENCE

Gold nanoparticle-mediated photoporation enables delivery of macromolecules over a wide range of molecular weights in human CD4+ T cells

Laurens RaesClarissa Van HeckeJulie MichielsStephan StremerschJuan FraireToon BransRanhua XiongStefaan De SmedtLinos VandekerckhoveKoen Raemdonck et al.

A1 Journal Article in CRYSTALS

High pressure nebulization (PIPAC) versus injection for the intraperitoneal administration of mRNA complexes

Molood ShariatiHeyang ZhangLeen Van de SandeBenedicte DescampsChristian VanhoveWouter WillaertWim CeelenStefaan De SmedtKatrien Remaut

A1 Journal Article in PHARMACEUTICAL RESEARCH

Improved label-free identification of individual exosome-like vesicles with Au@Ag nanoparticles as SERS substrate

Juan FraireStephan StremerschDavinia BouckaertTinne MonteyneThomas De BeerPieter WuytensRiet De RyckeAndre SkirtachKoen RaemdonckStefaan De Smedt et al.

A1 Journal Article in ACS APPLIED MATERIALS & INTERFACES

Laser-induced vapor nanobubbles improve diffusion in biofilms of antimicrobial agents for wound care

Eline TeirlinckJuan FraireHeleen Van AckerJasper WilleRosalie SwimbergheToon BransRanhua XiongMaarten MeireRoeland De MoorStefaan De Smedt et al.

A2 Journal Article in BIOFILM

Longitudinal in vivo assessment of host-microbe interactions in a murine model of pulmonary aspergillosis

Shweta SainiJennifer PoelmansHannelie KorfJames L. DooleySayuan LiangBella B. ManshianRein VerbekeStefaan J. SoenenGreetje Vande VeldeIne Lentacker et al.

A1 Journal Article in ISCIENCE

Morphology and composition of the inner limiting membrane : species-specific variations and relevance toward drug delivery research

Karen PeynshaertJoke DevoldereAn-Katrien MinnaertStefaan De SmedtKatrien Remaut

A1 Journal Article in CURRENT EYE RESEARCH

Müller cells as a target for retinal therapy

Joke DevoldereKaren PeynshaertStefaan De SmedtKatrien Remaut

A1 Journal Article in DRUG DISCOVERY TODAY

Nanocarrier lipid composition modulates the impact of pulmonary surfactant protein B (SP-B) on cellular delivery of siRNA

Roberta GuagliardoPieterjan MerckxAgata ZamborlinLynn De BackerMercedes EchaideJesus Pérez-GilStefaan De SmedtKoen Raemdonck

A1 Journal Article in PHARMACEUTICS

Non-viral delivery of chemically modified mRNA to the retina : subretinal versus intravitreal administration

Joke DevoldereKaren PeynshaertHeleen DewitteChristian VanhoveLies De GroefLieve MoonsSinem Yilmaz ÖzcanDeniz DalkaraStefaan De SmedtKatrien Remaut

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Photoablation of human vitreous opacities by light-induced vapor nanobubbles

Félix SauvageJuan FraireKatrien RemautJ SebagKaren PeynshaertMichael HarringtonFrans J Van de VeldeRanhua XiongMarie-Jose TassignonToon Brans et al.

A1 Journal Article in ACS NANO

Potential and pitfalls of non-viral mRNA delivery for ocular therapies

Joke DevoldereKatrien RemautStefaan De Smedt

Sonoprinting liposomes on tumor spheroids by microbubbles and ultrasound

Silke RooversJoke DeprezD PriwitaningrumG LajoinieN RivronHeidi DeclercqOlivier De WeverE StrideS Le GacM Versluis et al.

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Sonoprinting of nanomedicines : from mechanistic insights to preclinical translation

Silke RooversStefaan De SmedtIne Lentacker

Sonoprinting of nanoparticle-loaded microbubbles : unraveling the multi-timescale mechanism

Silke RooversGuillaume LajoinieIne De CockToon BransHeleen DewitteKevin BraeckmansMichel VersuisStefaan De SmedtIne Lentacker

A1 Journal Article in BIOMATERIALS

The obstacle course to the inner retina : hyaluronic acid-coated lipoplexes cross the vitreous but fail to overcome the inner limiting membrane

Joke DevoldereMike WelsKaren PeynshaertHeleen DewitteStefaan De SmedtKatrien Remaut

A1 Journal Article in EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS

The role of ultrasound-driven microbubble dynamics in drug delivery : from microbubble fundamentals to clinical translation

Silke RooversTim SegersGuillaume LajoinieJoke DeprezMichel VersluisStefaan De SmedtIne Lentacker

A1 Journal Article in LANGMUIR

Three decades of messenger RNA vaccine development

Rein VerbekeIne LentackerStefaan De SmedtHeleen Dewitte

A1 Journal Article in NANO TODAY

2018

Endosomal size and membrane leakiness influence proton sponge-based rupture of endosomal vesicles

Lotte VermeulenToon BransSangram Keshari SamalPeter DubruelJo DemeesterStefaan De SmedtKatrien RemautKevin Braeckmans

A1 Journal Article in ACS NANO

Fluorescence correlation spectroscopy to find the critical balance between extracellular association and intracellular dissociation of mRNA complexes

Heyang ZhangStefaan De SmedtKatrien Remaut

A1 Journal Article in ACTA BIOMATERIALIA

In vitro and ex vivo models to study drug delivery barriers in the posterior segment of the eye

Karen PeynshaertJoke DevoldereStefaan De SmedtKatrien Remaut

A1 Journal Article in ADVANCED DRUG DELIVERY REVIEWS

Laser-induced vapour nanobubbles improve drug diffusion and efficiency in bacterial biofilms

Eline TeirlinckRanhua XiongToon BransKatrien ForierJuan FraireHeleen Van AckerNele MatthijsRiet De RyckeStefaan De SmedtTom Coenye et al.

A1 Journal Article in NATURE COMMUNICATIONS

Methodologies to investigate intracellular barriers for nucleic acid delivery in non-viral gene therapy

Lotte VermeulenToon BransStefaan De SmedtKatrien RemautKevin Braeckmans

A1 Journal Article in NANO TODAY

Nanomaterials and molecular transporters to overcome the bacterial envelope barrier : towards advanced delivery of antibiotics

Rita SantosCéu FigueiredoNuno F. AzevedoKevin BraeckmansStefaan De Smedt

A1 Journal Article in ADVANCED DRUG DELIVERY REVIEWS

Nucleic acid loading and fluorescent labeling of isolated extracellular vesicles requires adequate purification

Stephan StremerschToon BransKevin BraeckmansStefaan De SmedtKoen Raemdonck

A1 Journal Article in INTERNATIONAL JOURNAL OF PHARMACEUTICS

Optimized messenger RNA immunolipoplexes for cancer immunotherapy : balancing immunogenicity and adjuvancy

Rein VerbekeIne LentackerKarine BreckpotSerge Van CalenberghStefaan De SmedtHeleen Dewitte

Photothermally triggered endosomal escape and its influence on transfection efficiency of gold-functionalized JetPEI/pDNA nanoparticles

Lotte VermeulenJuan FraireLaurens RaesEllen De MeesterSarah De KeulenaerFilip Van NieuwerburghStefaan De SmedtKatrien RemautKevin Braeckmans

A1 Journal Article in INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Pulmonary surfactant and drug delivery : focusing on the role of surfactant proteins

Roberta GuagliardoJésus Pérez-GilStefaan De SmedtKoen Raemdonck

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Quantifying the average number of nucleic acid therapeutics per nanocarrier by single particle tracking microscopy

Elisa ZagatoLotte VermeulenHeleen DewitteGriet Van ImschootRoosmarijn VandenbrouckeJo DemeesterStefaan De SmedtKristiaan NeytsKatrien RemautKevin Braeckmans

A1 Journal Article in MOLECULAR PHARMACEUTICS

Repeated photoporation with graphene quantum dots enables homogeneous labeling of live cells with extrinsic markers for fluorescence microscopy

Jing LiuRanhua XiongToon BransSaskia LippensEef ParthoensFrancesca Cella ZanacchiRaffaella MagrassiSantosh K SinghSreekumar KurungotSabine Szunerits et al.

A1 Journal Article in LIGHT-SCIENCE & APPLICATIONS

Repurposing cationic amphiphilic drugs as adjuvants to induce lysosomal siRNA escape in nanogel transfected cells

Freya JorisLynn De BackerThijs Van de VyverChiara BastiancichStefaan De SmedtKoen Raemdonck

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Selective labeling of individual neurons in dense cultured networks with nanoparticle-enhanced photoporation

Ranhua XiongPeter VerstraelenJo DemeesterAndre SkirtachJean-Pierre TimmermansStefaan De SmedtWinnok De VosKevin Braeckmans

A1 Journal Article in FRONTIERS IN CELLULAR NEUROSCIENCE

Surfactant protein B (SP-B) enhances the cellular siRNA delivery of proteolipid coated nanogels for inhalation therapy

Pieterjan MerckxLynn De BackerLien Van HoeckeRoberta GuagliardoMercedes EchaidePieter BaatsenBárbara OlmedaXavier SaelensJésus Pérez-GilStefaan De Smedt et al.

A1 Journal Article in ACTA BIOMATERIALIA

Targeted perturbation of nuclear envelope integrity with vapor nanobubble-mediated photoporation

Gaëlle HouthaeveRanhua XiongJoke RobijnsBert LuyckxYasmine BeulqueToon BransCoen CampsteijnSangram Keshari SamalStephan StremerschStefaan De Smedt et al.

A1 Journal Article in ACS NANO

Technical implementations of light sheet microscopy

Elisa ZagatoToon BransStefaan De SmedtKatrien RemautKristiaan NeytsKevin Braeckmans

A1 Journal Article in MICROSCOPY RESEARCH AND TECHNIQUE

The proton sponge hypothesis : fable or fact?

Lotte VermeulenStefaan De SmedtKatrien RemautKevin Braeckmans

A1 Journal Article in EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS

What you see is what you get : the physicochemistry of nanoparticles dictates how they cross ocular delivery barriers and alter autophagy

Karen PeynshaertKatrien RemautStefaan De Smedt

2017

Choose your cell model wisely : the in vitro nanoneurotoxicity of differentially coated iron oxide nanoparticles for neural cell labeling

Freya JorisDaniel ValdepérezBeatriz PelazTianqiang WangShareen H. DoakBella B. ManshianStefaan J. SoenenWolfgang J. ParakStefaan De SmedtKoen Raemdonck

A1 Journal Article in ACTA BIOMATERIALIA

Co-delivery of nucleoside-modified mRNA and TLR agonists for cancer immunotherapy : restoring the immunogenicity of immunosilent mRNA

Rein VerbekeIne LentackerLaura WayteckKarine BreckpotMieke Van BockstalBenedicte DescampsChristian VanhoveStefaan De SmedtHeleen Dewitte

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Co-delivery of nucleoside-modified mRNA and immunomodulators for cancer immunotherapy: restoring the immunogenicity of immunosilent mRNA

Rein VerbekeIne LentackerLaura WayteckKarine BreckpotBenedicte DescampsChristian VanhoveStefaan De SmedtHeleen Dewitte

Coating of quantum dots strongly defines their effect on lysosomal health and autophagy

Karen PeynshaertStefaan J SoenenBella B ManshianShareen H DoakKevin BraeckmansStefaan De SmedtKatrien Remaut

A1 Journal Article in ACTA BIOMATERIALIA

Comparing photoporation and nucleofection for delivery of small interfering RNA to cytotoxic T cells

Laura WayteckRanhua XiongKevin BraeckmansStefaan De SmedtKoen Raemdonck

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Effect of hyaluronic acid-binding to lipoplexes on intravitreal drug delivery for retinal gene therapy

Thomas MartensKaren PeynshaertThaís Leite NascimentoElias FattalMarcus KarlstetterThomas LangmannSerge PicaudJo DemeesterStefaan De SmedtKatrien Remaut et al.

A1 Journal Article in EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES

Exploring the HYDRAtion method for loading siRNA on liposomes : the interplay between stability and biological activity in human undiluted ascites fluid

George DakwarKevin BraeckmansWim CeelenStefaan De SmedtKatrien Remaut

A1 Journal Article in DRUG DELIVERY AND TRANSLATIONAL RESEARCH

Fast spatial-selective delivery into live cells

Ranhua XiongClaire DrullionPeter VerstraelenJo DemeesterAndre SkirtachCorinne AbbadieWinnok De VosStefaan De SmedtKevin Braeckmans

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

High-pressure nebulization as application route for the peritoneal administration of siRNA complexes

An-Katrien MinnaertGeorge DakwarJuan M BenitoJosé M García FernándezWim CeelenStefaan De SmedtKatrien Remaut

A1 Journal Article in MACROMOLECULAR BIOSCIENCE

In vitro and ex vivo delivery of tailored siRNA-nanoliposomes for E2F1 silencing as a potential therapy for colorectal cancer

Sabrina BochicchioBarbara DapasIlaria RussoCarolina CiacciOrnella PiazzaStefaan De SmedtEline PottieAnna Angela BarbaGabriele Grassi

A1 Journal Article in INTERNATIONAL JOURNAL OF PHARMACEUTICS

Intracellular delivery of biopharmaceuticals and contrast agents by VNB photoporation and sizing nanomaterials in bio-fluids by FRAP

Ranhua XiongKevin BraeckmansStefaan De SmedtAndre Skirtach

Intracellular delivery of oligonucleotides in Helicobacter pylori by fusogenic liposomes in the presence of gastric mucus

Rita SantosGeorge DakwarElisa ZagatoToon BransCéu FigueiredoKoen RaemdonckNuno F AzevedoStefaan De SmedtKevin Braeckmans

A1 Journal Article in BIOMATERIALS

Microfabricated devices for single objective single plane illumination microscopy (SoSPIM)

Elisa ZagatoToon BransSteven VerstuyftDries Van ThourhoutJeroen MissinneGeert Van SteenbergeJo DemeesterStefaan De SmedtKatrien RemautKristiaan Neyts et al.

A1 Journal Article in OPTICS EXPRESS

Nanomedicine-based intraperitoneal therapy for the treatment of peritoneal carcinomatosis : mission possible?

George DakwarMolood ShariatiWouter WillaertWim CeelenStefaan De SmedtKatrien Remaut

A1 Journal Article in ADVANCED DRUG DELIVERY REVIEWS

PEGylated and functionalized aliphatic polycarbonate polyplex nanoparticles for intravenous administration of HDAC5 siRNA in cancer therapy

Antoine FrèreAlexandra BaroniElodie HendrickAnne-Sophie DelvigneFrançois OrangeOlivier PeulenGeorge DakwarJérôme DiricqPhilippe DuboisBrigitte Evrard et al.

A1 Journal Article in ACS APPLIED MATERIALS & INTERFACES

Polyimide/cellulose acetate core/shell electrospun fibrous membranes for oil-water separation

Wenjing MaZhongfu GuoJuntao ZhaoQian YuFang WangJingquan HanHui PanJianfeng YaoQilu ZhangSangram Keshari Samal et al.

A1 Journal Article in SEPARATION AND PURIFICATION TECHNOLOGY

Small molecules convey big messages : boosting non-viral nucleic acid delivery with low molecular weight drugs

Freya JorisStefaan De SmedtKoen Raemdonck

A1 Journal Article in NANO TODAY

The repurposing of low molecular weight drugs to induce lysosomal release of siRNA and in vitro approaches to probe the toxicity of inorganic nanoparticles

Freya JorisStefaan De SmedtKoen Raemdonck

The role of intracellular trafficking of CdSe/ZnS QDs on their consequent toxicity profile

Bella B ManshianThomas MartensKarsten KantnerKevin BraeckmansStefaan De SmedtJo DemeesterGareth JS JenkinsWolfgang J ParakBeatriz PelazShareen H Doak et al.

A1 Journal Article in JOURNAL OF NANOBIOTECHNOLOGY

Toward smart design of retinal drug carriers : a novel bovine retinal explant model to study the barrier role of the vitreoretinal interface

Karen PeynshaertJoke DevoldereValérie ForsterSerge PicaudChristian VanhoveStefaan De SmedtKatrien Remaut

A1 Journal Article in DRUG DELIVERY

Unraveling the potential of nucleic acid mimics to target Helicobacter pylori infections : the effect of biological barriers

Rita SantosNuno F AzevedoKevin BraeckmansStefaan De SmedtCéu Figueiredo

mRNA-based nanoparticles for therapeutic cancer vaccination

Rein VerbekeLaura WayteckKarine BreckpotChristian VanhoveBenedicte DescampsStefaan De SmedtHeleen DewitteIne Lentacker

2016

Combining the strengths of cytotoxic T cells and nanomedicines for cancer immunotherapy

Laura WayteckStefaan De SmedtKoen Raemdonck

Comparing exosome-like vesicles with liposomes for the functional cellular delivery of small RNAs

Stephan StremerschRoosmarijn VandenbrouckeElien Van WonterghemAn HendrixStefaan De SmedtKoen Raemdonck

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Core-sheath structured electrospun nanofibrous membranes for oil-water separation

Wenjing MaQilu ZhangSangram Keshari SamalFang WangBuhong GaoHui PanHaijun XuJianfeng YaoXianxu ZhanStefaan De Smedt et al.

A1 Journal Article in RSC ADVANCES

Cytosolic delivery of nanolabels prevents their asymmetric inheritance and enables extended quantitative in vivo cell imaging

Ranhua XiongFreya JorisSayuan LiangRiet De RyckeSaskia LippensJo DemeesterAndre SkirtachKoen RaemdonckUwe HimmelreichStefaan De Smedt et al.

A1 Journal Article in NANO LETTERS

Evading innate immunity in nonviral mRNA delivery : don't shoot the messenger

Joke DevoldereHeleen DewitteStefaan De SmedtKatrien Remaut

A1 Journal Article in DRUG DISCOVERY TODAY

Exploring extracellular vesicles for siRNA delivery and Raman-based diagnostics

Stephan StremerschStefaan De SmedtKoen RaemdonckKevin Braeckmans

Exploring the intraperitoneal route of administration for siRNA delivery in the treatment of pertitoneal carcinomatosis

George DakwarKatrien RemautStefaan De SmedtWim Ceelen

Hitchhiking nanoparticles: reversible coupling of lipid-based nanoparticles to cytotoxic T lymphocytes

Laura WayteckHeleen DewitteLynn De BackerKarine BreckpotJo DemeesterStefaan De SmedtKoen Raemdonck

A1 Journal Article in BIOMATERIALS

Identification of individual exosome-like vesicles by surface enhanced Raman spectroscopy

Stephan StremerschMonica MarroBat-El PinchasikPieter BaatsenAn HendrixStefaan De SmedtPablo Loza-AlvarezAndre SkirtachKoen RaemdonckKevin Braeckmans

A1 Journal Article in SMALL

Laser-assisted photoporation : fundamentals, technological advances and applications

Ranhua XiongSangram Keshari SamalJo DemeesterAndre SkirtachStefaan De SmedtKevin Braeckmans

A1 Journal Article in ADVANCES IN PHYSICS-X

Sizing nanomaterials in bio-fluids by cFRAP enables protein aggregation measurements and diagnosis of bio-barrier permeability

Ranhua XiongRoosmarijn VandenbrouckeKatleen BroosToon BransElien Van WonterghemClaude LibertJo DemeesterStefaan De SmedtKevin Braeckmans

A1 Journal Article in NATURE COMMUNICATIONS

Sonoprinting and the importance of microbubble loading for the ultrasound mediated cellular delivery of nanoparticles

Ine De CockGuillaume LajoinieMichel VersluisStefaan De SmedtIne Lentacker

A1 Journal Article in BIOMATERIALS

The impact of species and cell type on the nanosafety profile of iron oxide nanoparticles in neural cells

Freya JorisDaniel ValdepérezBeatriz PelazStefaan J SoenenBella B ManshianWolfgang J ParakStefaan De SmedtKoen Raemdonck

A1 Journal Article in JOURNAL OF NANOBIOTECHNOLOGY

Therapeutic and diagnostic applications of extracellular vesicles

Stephan StremerschStefaan De SmedtKoen Raemdonck

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Unraveling microbubble-cell interactions and drug delivery mechanisms in ultrasound-guided therapy

Ine De CockStefaan De SmedtIne Lentacker

pH responsive polyurethane (core) and cellulose acetate phthalate (shell) electrospun fibers for intravaginal drug delivery

Dawei HuaZhongche LiuFang WangBuhong GaoFei ChenQilu ZhangRanhua XiongJingquan HanSangram Keshari SamalStefaan De Smedt et al.

A1 Journal Article in CARBOHYDRATE POLYMERS

2015

Bio-inspired materials in drug delivery : exploring the role of pulmonary surfactant in siRNA inhalation therapy

Lynn De BackerAlejandro CerradaJesús Pérez-GilStefaan De SmedtKoen Raemdonck

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Bio-inspired pulmonary surfactant-modified nanogels : a promising siRNA delivery system

Lynn De BackerKevin BraeckmansMarc CA StuartJo DemeesterStefaan De SmedtKoen Raemdonck

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Biomimetic magnetic silk scaffolds

Sangram Keshari SamalMamoni DashTatiana ShelyakovaHeidi DeclercqMarc UhlarzManuel Banobre-LopezPeter DubruelMaria CornelissenThomas HerrmannsdorferJose Rivas et al.

A1 Journal Article in ACS APPLIED MATERIALS & INTERFACES

Coating nanocarriers with hyaluronic acid facilitates intravitreal drug delivery for retinal gene therapy

Thomas MartensKatrien RemautHendrik DeschoutJohan FJ EngbersenWim E HenninkMies J van SteenbergenJo DemeesterStefaan De SmedtKevin Braeckmans

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Design and evaluation of immunotherapeutic imaging for cancer vaccination

Heleen DewitteStefaan De SmedtKarine BreckpotIne Lentacker

Disregarded effect of biological fluids in siRNA delivery : human ascites fluid severely restricts cellular uptake of nanoparticles

George DakwarKevin BraeckmansJo DemeesterWim CeelenStefaan De SmedtKatrien Remaut

A1 Journal Article in ACS APPLIED MATERIALS & INTERFACES

Effect of native gastric mucus on in vivo hybridization therapies directed at Helicobacter pylori

Rita SantosGeorge DakwarRanhua XiongKatrien ForierKatrien RemautStephan StremerschNuno GuimarãesSilvia FonteneteJesper WengelMarina Leite et al.

A1 Journal Article in MOLECULAR THERAPY-NUCLEIC ACIDS

Efficient delivery of quantum dots in live cells by gold nanoparticle mediated photoporation

Ranhua XiongFreya JorisIne De CockJo DemeesterStefaan De SmedtAndre SkirtachKevin Braeckmans

Evaluation of mRNA lipoplexes for single-step antigen loading and maturation of dendritic cells in cancer immunotherapy

Rein VerbekeHeleen DewitteIne LentackerStefaan De Smedt

Exploring bio-inspired nanocomposites of nanogels and pulmonary surfactant for small interfering RNA delivery

Lynn De BackerStefaan De SmedtKoen Raemdonck

Hybrid pulmonary surfactant-coated nanogels mediate efficient in vivo delivery of siRNA to murine alveolar macrophages

Lynn De BackerThomas NaessensStefaan De KokerElisa ZagatoJo DemeesterJohan GrootenStefaan De SmedtKoen Raemdonck

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Immunogenic mRNA nanoparticles for dendritic cell-based cancer vaccination

Rein VerbekeHeleen DewitteIne LentackerStefaan De Smedt

Intraperitoneal nonviral nucleic acid delivery in the treatment of peritoneal cancer

George DakwarStefaan De SmedtKatrien Remaut

Bookchapter in Intraperitoneal cancer therapy : principles and practice

Laser-induced vapor nanobubbles for efficient delivery of macromolecules in live cells

Ranhua XiongKoen RaemdonckKaren PeynshaertIne LentackerIne De CockJo DemeesterStefaan De SmedtAndre SkirtachKevin Braeckmans

Lessons in simplicity that should shape the future of drug delivery

Koen RaemdonckStefaan De Smedt

A1 Journal Article in NATURE BIOTECHNOLOGY

Microbubbles as magic bullets in cancer immunotherapy

Heleen DewitteSandra Van LintKatrien VanderperrenHendrik HaersEmmelie StockStefaan De SmedtKarine BreckpotIne Lentacker

Multilayered magnetic gelatin membrane scaffolds

Sangram Keshari SamalVitaly GoranovMamoni DashAlessandro RussoTatiana ShelyakovaPatrizio GraziosiLisa LungaroAlberto RiminucciMarc UhlarzManuel Banobre-Lopez et al.

A1 Journal Article in ACS APPLIED MATERIALS & INTERFACES

N-1-methylpseudouridine-incorporated mRNA outperforms pseudouridine-incorporated mRNA by providing enhanced protein expression and reduced immunogenicity in mammalian cell lines and mice

Oliwia AndriesSéan Mc CaffertyStefaan De SmedtRon WeissNiek SandersTasuku Kitada

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

The effect of intracellular degradation on cytotoxicity and cell labeling efficacy of inorganic ligand-stabilized colloidal CdSe/CdS quantum dots

Stefaan SoenenSofie AbéBella B ManshianTangi AubertZeger HensStefaan De SmedtKevin Braeckmans

A1 Journal Article in JOURNAL OF BIOMEDICAL NANOTECHNOLOGY

Theranostic mRNA-loaded microbubbles in the lymphatics of dogs: implications for drug delivery

Heleen DewitteKatrien VanderperrenHendrik HaersEmmelie StockLuc DuchateauMyriam HestaJimmy SaundersStefaan De SmedtIne Lentacker

A1 Journal Article in THERANOSTICS

Transport mechanisms of squalenoyl-adenosine nanoparticles across the blood-brain barrier

Alice GaudinOya TagitDunja SobotSinda Lepetre-MouelhiJulie MouginThomas F MartensKevin BraeckmansValerie NicolasDidier DesmaeleStefaan De Smedt et al.

A1 Journal Article in CHEMISTRY OF MATERIALS

Ultrasound and microbubble mediated drug delivery: acoustic pressure as determinant for uptake via membrane pores or endocytosis

Ine De CockElisa ZagatoKevin BraeckmansYing LuanNico de JongStefaan De SmedtIne Lentacker

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

mRNA Modification and delivery strategies towards the establishment of a platform for safe and effective gene therapy

Oliwia AndriesNiek SandersStefaan De SmedtTasuku Kitada

2014

A personalized view on cancer immunotherapy

Laura WayteckKarine BreckpotJo DemeesterStefaan De SmedtKoen Raemdonck

A1 Journal Article in CANCER LETTERS

Cell division responsive peptides for optimized plasmid DNA delivery : the mitotic window of opportunity?

Katrien RemautNathalie SymensBart LucasJo DemeesterStefaan De Smedt

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Chitosan nanoparticles for siRNA delivery : optimizing formulation to increase stability and efficiency

H RagelleR RivaG VandermeulenBroes NaeyeV PourcelleCS Le DuffC D'HaeseB NystenKevin BraeckmansStefaan De Smedt et al.

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Choose your models wisely: how different murine bone marrow-derived dendritic cell protocols influence the success of nanoparticulate vaccines in vitro

Heleen DewitteRein VerbekeKarine BreckpotRoosmarijn VandenbrouckeClaude LibertStefaan De SmedtIne Lentacker

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Colloidal stability of nano-sized particles in the peritoneal fluid : towards optimizing drug delivery systems for intraperitoneal therapy

George DakwarElisa ZagatoJoris DelangheSabina HobelAchim AignerHannelore DenysKevin BraeckmansWim CeelenStefaan De SmedtKatrien Remaut

A1 Journal Article in ACTA BIOMATERIALIA

Comparison of gold nanoparticle mediated photoporation: vapor nanobubbles outperform direct heating for delivering macromolecules in live cells

Ranhua XiongKoen RaemdonckKaren PeynshaertIne LentackerIne De CockJo DemeesterStefaan De SmedtAndre SkirtachKevin Braeckmans

A1 Journal Article in ACS NANO

Cytotoxicity of cadmium-free quantum dots and their use in cell bioimaging

Stefaan SoenenBella B ManshianTangi AubertUwe HimmelreichJo DemeesterStefaan De SmedtZeger HensKevin Braeckmans

A1 Journal Article in CHEMICAL RESEARCH IN TOXICOLOGY

Effect of covalent fluorescence labeling of plasmid DNA on its intracellular processing and transfection with lipid-based carriers

Koen RomboutsThomas MartensElisa ZagatoJo DemeesterStefaan De SmedtKevin BraeckmansKatrien Remaut

A1 Journal Article in MOLECULAR PHARMACEUTICS

Electrospun polystyrene fibers for HIV entrapment

Chaobo HuangStefaan SoenenEllen van GulckJoanna RejmanGuido VanhamBart LucasBart GeersKevin BraeckmansVictor ShahinPieter Spanoghe et al.

A1 Journal Article in POLYMERS FOR ADVANCED TECHNOLOGIES

Exploiting intrinsic nanoparticle toxicity: the pros and cons of nanoparticle-induced autophagy in biomedical research

Karen PeynshaertBella Barjouhi ManshianFreya JorisKevin BraeckmansStefaan De SmedtJo DemeesterStefaan Soenen

A1 Journal Article in CHEMICAL REVIEWS

FRAP in pharmaceutical research: practical guidelines and applications in drug delivery

Hendrik DeschoutKoen RaemdonckJo DemeesterStefaan De SmedtKevin Braeckmans

A1 Journal Article in PHARMACEUTICAL RESEARCH

Intracellular delivery of nanomaterials: how to catch endosomal escape in the act

Thomas MartensKatrien RemautJo DemeesterStefaan De SmedtKevin Braeckmans

A1 Journal Article in NANO TODAY

Lipid and polymer nanoparticles for drug delivery to bacterial biofilms

Katrien ForierKoen RaemdonckStefaan De SmedtJo DemeesterTom CoenyeKevin Braeckmans

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Lysosomal capturing of cytoplasmic injected nanoparticles by autophagy: an additional barrier to non viral gene delivery

Katrien RemautViola OorschotKevin BraeckmansJudith KlumpermanStefaan De Smedt

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Magnetic layer-by-layer coated particles for efficient MRI of dendritic cells and mesenchymal stem cells

Marie-Luce De TemmermanStefaan SoenenNathalie SymensBart LucasRoosmarijn VandenbrouckeClaude LibertJo DemeesterStefaan De SmedtUwe HimmelreichJoanna Rejman

A1 Journal Article in NANOMEDICINE

Merging the best of both worlds : hybrid lipid-enveloped matrix nanocomposites in drug delivery

Koen RaemdonckKevin BraeckmansJo DemeesterStefaan De Smedt

A1 Journal Article in CHEMICAL SOCIETY REVIEWS

Nanoparticle design to induce tumor immunity and challenge the suppressive tumor microenvironment

Heleen DewitteRein VerbekeKarine BreckpotStefaan De SmedtIne Lentacker

A1 Journal Article in NANO TODAY

On-chip light sheet illumination enables diagnostic size and concentration measurements of membrane vesicles in biofluids

Hendrik DeschoutKoen RaemdonckStephan StremerschPietro MaoddiGuillaume MernierPhilippe RenaudSebastien JiguetAn Hendrixmarc brackeRudy Van den Broecke et al.

A1 Journal Article in NANOSCALE

Optimizing delivery of gene nanomedicines towards the retina via intravitreal administration

Thomas MartensKevin BraeckmansStefaan De SmedtKatrien Remaut

Probing the size limit for nanomedicine penetration into Burkholderia multivorans and Pseudomonas aeruginosa biofilms

Katrien ForierAnne-Sophie MessiaenKoen RaemdonckHans NelisStefaan De SmedtJo DemeesterTom CoenyeKevin Braeckmans

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Single-particle tracking for studying nanomaterial dynamics: applications and fundamentals in drug delivery

Elisa ZagatoKatrien ForierThomas MartensKristiaan NeytsJo DemeesterStefaan De SmedtKatrien RemautKevin Braeckmans

A1 Journal Article in NANOMEDICINE

The bubble breakthrough in cancer immunotherapy

Heleen DewitteSandra Van LintKatrien VanderperrenStefaan De SmedtKarine BreckpotIne Lentacker

The bubble breakthrough in cancer immunotherapy : Mma-sonoporation in dendritic cell-based cancer vaccination

Heleen DewitteSandra Van LintKatrien VanderperrenKris ThielemansStefaan De SmedtKarine BreckpotIne Lentacker

The bubble breakthrough in cancer immunotherapy : Mma-sonoporation in dendritic cell-based cancer vaccination

Heleen DewitteSandra Van LintKatrien VanderperrenKris ThielemansStefaan De SmedtKarine BreckpotIne Lentacker

The cellular interactions of PEGylated gold nanoparticles : effect of PEGylation on cellular uptake and cytotoxicity

Stefaan SoenenBella B ManshianAbuelmagd M AbdelmonemJosé-María MontenegroSisareuth TanLieve BalcaenFrank VanhaeckeAlain R BrissonWolfgang J ParakStefaan De Smedt et al.

A1 Journal Article in PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION

The effect of nanoparticle degradation on poly(methacrylic acid)-coated quantum dot toxicity : the importance of particle functionality assessment in toxicology

Stefaan SoenenJose-Maria MontenegroAbuelmagd AbdelmonemBella ManshianShareen DoakWolfgang ParakStefaan De SmedtKevin Braeckmans

A1 Journal Article in ACTA BIOMATERIALIA

The performance of gradient alloy quantum dots in cell labeling

Stefaan SoenenBella B ManshianUwe HimmelreichJo DemeesterKevin BraeckmansStefaan De Smedt

A1 Journal Article in BIOMATERIALS

The potential of antigen and TriMix sonoporation using mRNA-loaded microbubbles for ultrasound-triggered cancer immunotherapy

Heleen DewitteSandra Van LintCarlo HeirmanKris ThielemansStefaan De SmedtKarine BreckpotIne Lentacker

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Understanding ultrasound induced sonoporation: definitions and underlying mechanisms

Ine LentackerIne De CockR DeckersStefaan De SmedtCTW Moonen

A1 Journal Article in ADVANCED DRUG DELIVERY REVIEWS

Unraveling the effects of siRNA carrier systems on cell physiology: a multiparametric approach demonstrated on dextran nanogels

Stefaan SoenenLynn De BackerBella ManshianShareen DoakKoen RaemdonckJo DemeesterKevin BraeckmansStefaan De Smedt

A1 Journal Article in NANOMEDICINE

2013

Assessing nanoparticle toxicity in cell-based assays : influence of cell culture parameters and optimized models for bridging the in vitro-in vivo gap

Freya JorisBella B ManshianKaren PeynshaertStefaan De SmedtKevin BraeckmansStefaan Soenen

A1 Journal Article in CHEMICAL SOCIETY REVIEWS

Comparison of in vivo optical systems for bioluminescence and fluorescence imaging

Steven CoolKoen BreyneEvelyne MeyerStefaan De SmedtNiek Sanders

A1 Journal Article in JOURNAL OF FLUORESCENCE

Correlation of dual colour single particle trajectories for improved detection and analysis of interactions in living cells

Hendrik DeschoutThomas MartensDries VercauterenKatrien RemautJo DemeesterStefaan De SmedtKristiaan NeytsKevin Braeckmans

A1 Journal Article in INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Coupling of drug containing liposomes to microbubbles improves ultrasound triggered drug delivery in mice

Steven CoolBart GeersStefan RoelsStephan StremerschKatrien VanderperrenJimmy SaundersStefaan De SmedtJo DemeesterNiek Sanders

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Current methods for inducing pluripotency in somatic cells

Geertrui TavernierBarbara MlodyJo DemeesterJames AdjayeStefaan De Smedt

A1 Journal Article in ADVANCED MATERIALS

Design and evaluation of theranostic perfluorocarbon particles for simultaneous antigen-loading and 19F-MRI tracking of dendritic cells

Heleen DewitteBart GeersSayuan LiangUwe HimmelreichJo DemeesterStefaan De SmedtIne Lentacker

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Development of nanomedicines for the treatment of pulmonary biofilm infections: Insights from advanced fluorescence microscopy studies

Katrien ForierAnne-Sophie MessiaenMartijn RisseeuwSerge Van CalenberghHendrik DeschoutKoen RaemdonckHans NelisFabienne DanhierVéronique PréatFrans De Baets et al.

Electroporation-induced siRNA precipitation obscures the efficiency of siRNA loading into extracellular vesicles

Sander AA KooijmansStephan StremerschKevin BraeckmansStefaan De SmedtAn HendrixMatthew JA WoodRaymond M SchiffelersKoen RaemdonckPieter Vader

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Enhancing nucleic acid delivery with ultrasound and microbubbles

Steven CoolBart GeersIne LentackerStefaan De SmedtNiek Sanders

Bookchapter in Nanotechnology for nucleic acid delivery : methods and protocols

Fluorescent non-porous silica nanoparticles for long-term cell monitoring: cytotoxicity and particle functionality

Stefaan SoenenBella ManshianShareen H DoakeStefaan De SmedtKevin Braeckmans

A1 Journal Article in ACTA BIOMATERIALIA

Focal delivery of AAV2/1-transgenes into the rat brain by localized ultrasound-induced BBB opening

Angelika AlonsoEileen ReinzBarbara LeuchsJuergen KleinschmidtMarc FatarBart GeersIne LentackerMichael G. HennericiStefaan De SmedtStephen Meairs

A1 Journal Article in MOLECULAR THERAPY-NUCLEIC ACIDS

In vivo disassembly of IV administered siRNA matrix nanoparticles at the renal filtration barrier

Broes NaeyeHendrik DeschoutVicky CaveliersBenedicte DescampsKevin BraeckmansChristian VanhoveJo DemeesterTony LahoutteStefaan De SmedtKoen Raemdonck

A1 Journal Article in BIOMATERIALS

Injectable drug-loaded microbubbles : a magic bullet for image-guided cancer therapy?

Bart GeersStefaan De SmedtJo DemeesterIne Lentacker

Innate immune response and programmed cell death following carrier-mediated delivery of unmodified mRNA to respiratory cells

Oliwia AndriesMarina De FiletteStefaan De SmedtJo DemeesterMario Van PouckeLuc PeelmanNiek Sanders

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Light-addressable capsules as caged compound matrix for controlled triggering of cytosolic reactions

Markus OchsSusana Carregal-RomeroJoanna RejmanKevin BraeckmansStefaan De SmedtWolfgang Parak

A1 Journal Article in ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Lipoplexes carrying mRNA encoding Gag protein modulate dendritic cells to stimulate HIV-specific immune responses

Winni De HaesJoanna RejmanCharlotte PollardCéline MerlinMarc VekemansEric FlorenceStefaan De SmedtJohan GrootenGuido VanhamStefaan De Koker et al.

A1 Journal Article in NANOMEDICINE

Measuring the intravitreal mobility of nanomedicines with single-particle tracking microscopy

Thomas MartensDries VercauterenKatrien ForierHendrik DeschoutKatrien RemautRik PaesenMarcel AmelootJohan FJ EngbersenJo DemeesterStefaan De Smedt et al.

A1 Journal Article in NANOMEDICINE

Noncoding DNA in lipofection of HeLa cells: a few insights

Nathalie SymensJoanna RejmanBart LucasJo DemeesterStefaan De SmedtKatrien Remaut

A1 Journal Article in MOLECULAR PHARMACEUTICS

Polysaccharide-based nucleic acid nanoformulations

Koen RaemdonckThomas F MartensKevin BraeckmansJo DemeesterStefaan De Smedt

A1 Journal Article in ADVANCED DRUG DELIVERY REVIEWS

Rectangle FRAP for measuring diffusion with a laser scanning microscope

Ranhua XiongHendrik DeschoutJo DemeesterStefaan De SmedtKevin Braeckmans

Bookchapter in Fluorescence spectroscopy and microscopy : methods and protocols

Targeted liposome-loaded microbubbles for cell-specific ultrasound-triggered drug delivery

Bart GeersOlivier De WeverJo DemeesterMarc BrackeStefaan De SmedtIne Lentacker

A1 Journal Article in SMALL

The biocompatibility of nanosized materials: intracellular nanoparticle stability and effects on toxicity and particle functionality

Stefaan SoenenStefaan De SmedtKevin Braeckmans

The influence of natural pulmonary surfactant on the efficacy of siRNA-loaded dextran nanogels

Lynn De BackerKevin BraeckmansJo DemeesterStefaan De SmedtKoen Raemdonck

A1 Journal Article in NANOMEDICINE

Transport of nanoparticles in cystic fibrosis sputum and bacterial biofilms by single-particle tracking microscopy

Katrien ForierAnne-Sophie MessiaenKoen RaemdonckHendrik DeschoutJoanna RejmanFrans De BaetsHans NelisStefaan De SmedtJo DemeesterTom Coenye et al.

A1 Journal Article in NANOMEDICINE

Turning a frown upside down: exploiting nanoparticle toxicity for anticancer therapy

Stefaan SoenenJo DemeesterStefaan De SmedtKevin Braeckmans

A1 Journal Article in NANO TODAY

Type I IFN counteracts the induction of antigen-specific immune responses by lipid-based delivery of mRNA vaccines

Charlotte PollardJoanna RejmanWinni De HaesBernard VerrierEllen Van GulckThomas NaessensStefaan De SmedtPieter BogaertJohan GrootenGuido Vanham et al.

A1 Journal Article in MOLECULAR THERAPY

2012

Activation of pluripotency-associated genes in mouse embryonic fibroblasts by non-viral transfection with in vitro-derived mRNAs encoding Oct4, Sox2, Klf4 and cMyc

Geertrui TavernierKatharina WolfrumJo DemeesterStefaan De SmedtJames AdjayeJoanna Rejman

A1 Journal Article in BIOMATERIALS

An ex vivo assay to measure the mobility inside the eye of nanomedicines for retinal gene therapy

Thomas MartensDries VercauterenKatrien ForierHendrik DeschoutKatrien RemautJo DemeesterStefaan De SmedtKevin Braeckmans

Bio-inspired surfactant-modified dextran nanogels mediate enhanced intracellular siRNA delivery

Lynn De BackerJo DemeesterStefaan De SmedtKoen Raemdonck

Comparison of polymeric siRNA nanocarriers in a murine LPS-activated macrophage cell line: gene silencing, toxicity and off-target gene expression

Linda B JensenJoscha GrigerBroes NaeyeAmir K VarkouhiKoen RaemdonckRaymond SchiffelersTwan LammersGert StormStefaan De SmedtBrian S Sproat et al.

A1 Journal Article in PHARMACEUTICAL RESEARCH

Comparison of the gene transfer efficiency of mRNA/GL67 and pDNA/GL67 complexes in respiratory cells

Oliwia AndriesMarina De FiletteJoanna RejmanStefaan De SmedtJo DemeesterMario Van PouckeLuc PeelmanCindy PelemanTony LahoutteNiek Sanders

A1 Journal Article in MOLECULAR PHARMACEUTICS

Crucial factors and emerging concepts in ultrasound-triggered drug delivery

Bart GeersHeleen DewitteStefaan De SmedtIne Lentacker

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Cytotoxic effects of gold nanoparticles : a multiparametric study

Stefaan SoenenBella ManshianJosé María MontenegroFaheem AminBjörn MeermannToke ThironMaria CornelissenFrank VanhaeckeShareen DoakWolfgang J Parak et al.

A1 Journal Article in ACS NANO

Delivery vehicles carrying antigens to dendritic cells

Marie-Luce De TemmermanStefaan De SmedtJo DemeesterJoanna Rejman

Efficient transfection of hepatocytes mediated by mRNA complexed to galactosylated cyclodextrins

Nathalie SymensAlejandro Méndez-ArdoyAlejandro Diaz-MoscosoElena Sánchez-FernéndezKatrien RemautJo DemeesterJosé M García FernándezStefaan De SmedtJoanna Rejman

A1 Journal Article in BIOCONJUGATE CHEMISTRY

Electrospun cellulose acetate phthalate fibers for semen induced anti-HIV vaginal drug delivery

Chaobo HuangStefaan SoenenEllen van GulckGuido VanhamJoanna RejmanSerge Van CalenberghChris VervaetTom CoenyeHans VerstraelenMarleen Temmerman et al.

A1 Journal Article in BIOMATERIALS

Elucidating the pre- and post-nuclear intracellular processing of 1,4-dihydropyridine based gene delivery carriers

Zanna HyvönenVesa HämäläinenMarika RuponenBart LucasJoanna RejmanDries VercauterenJo DemeesterStefaan De SmedtKevin Braeckmans

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Evaluation of small interfering RNA delivery by nanoparticle-loaded tumoritropic cells

Laura WayteckStefaan De SmedtKoen Raemdonck

Internalization of mRNA lipoplexes by dendritic cells

Winni De HaesGreet Van MolCéline MerlinStefaan De SmedtGuido VanhamJoanna Rejman

A1 Journal Article in MOLECULAR PHARMACEUTICS

Intracellular drug delivery in Leishmania-infected macrophages: evaluation of saponin-loaded PLGA nanoparticles

H Van De VenM VermeerschRoosmarijn VandenbrouckeA MatheeussenS ApersW WeyenbergStefaan De SmedtP CosL MaesA Ludwig

A1 Journal Article in JOURNAL OF DRUG TARGETING

Intracellular partitioning of cell organelles and extraneous nanoparticles during mitosis

Nathalie SymensStefaan SoenenJoanna RejmanKevin BraeckmansStefaan De SmedtKatrien Remaut

A1 Journal Article in ADVANCED DRUG DELIVERY REVIEWS

Investigating the toxic effects of iron oxide nanoparticles

Stefaan SoenenMarcel De CuyperStefaan De SmedtKevin Braeckmans

A1 Journal Article in Methods in Enzymology

Limitations and caveats of magnetic cell labeling using transfection agent complexed iron oxide nanoparticles

Stefaan SoenenStefaan De SmedtKevin Braeckmans

A1 Journal Article in CONTRAST MEDIA & MOLECULAR IMAGING

Lipo- and polyplex-mediated mRNA delivery and its application in reprogramming of fibroblasts towards pluripotency

Geertrui TavernierStefaan De SmedtJoanna RejmanJames Adjaye

Liposome based systems for systemic siRNA delivery: stability in blood sets the requirements for optimal carrier design

Kevin BuyensStefaan De SmedtKevin BraeckmansJo DemeesterLiesbeth PeetersLeo A van GrunsvenXavier de Mollerat du JeuRupa SawantVladimir TorchilinKatarina Farkasova et al.

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Magnetic electrospun fibers for cancer therapy

Chaobo HuangStefaan SoenenJoanna RejmanJesse TrekkerLiu ChengxunLiesbet LagaeWim CeelenClaire WilhelmJo DemeesterStefaan De Smedt

A1 Journal Article in ADVANCED FUNCTIONAL MATERIALS

Matrix systems for siRNA delivery

Broes NaeyeKoen RaemdonckKatrien RemautJo DemeesterStefaan De Smedt

A1 Journal Article in CURRENT TOPICS IN MEDICINAL CHEMISTRY

Modulation of dendritic cells by lipid grafted polyelectrolyte microcapsules

Marie-Luce De TemmermanJoanna RejmanBart LucasRoosmarijn VandenbrouckeClaude LibertJo DemeesterStefaan De Smedt

A1 Journal Article in ADVANCED FUNCTIONAL MATERIALS

On the cellular processing of non-viral nanomedicines for nucleic acid delivery: mechanisms and methods

Dries VercauterenJoanna RejmanThomas MartensJo DemeesterStefaan De SmedtKevin Braeckmans

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Overcoming intracellular barriers in non-viral gene delivery : chromatin targeting, messenger RNA and non-coding DNA

Nathalie SymensStefaan De SmedtKatrien RemautJoanna Rejman

Polyelectrolyte LbL microcapsules versus PLGA microparticles for immunization with a protein antigen

Marie-Luce De TemmermanJoanna RejmanRoosmarijn VandenbrouckeStefaan De KokerClaude LibertJohan GrootenJo DemeesterBruno GanderStefaan De Smedt

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Tailoring layer-by-layer capsules for biomedical applications

Marie-Luce De TemmermanJo DemeesterStefaan De SmedtJoanna Rejman

A1 Journal Article in NANOMEDICINE

The cytotoxic and immunogenic hurdles associated with non-viral mRNA-mediated reprogramming of human fibroblasts

Katharina DrewsGeertrui TavernierJo DemeesterHans LehrachStefaan De SmedtJoanna RejmanJames Adjaye

A1 Journal Article in BIOMATERIALS

The cytotoxic effects of polymer-coated quantum dots and restrictions for live cell applications

Stefaan SoenenJo DemeesterStefaan De SmedtKevin Braeckmans

A1 Journal Article in BIOMATERIALS

The mechanisms behind sonoporation through the eyes of an AAV-vector

Bart GeersIne LentackerAngelika AlonsoNiek SandersJo DemeesterStephen MeairsStefaan De Smedt

A2 Journal Article in PUBLICATIONS QUARTERLY / AMERICAN CHEMICAL SOCIETY

siRNA and pharmacological inhibition of endocytic pathways to characterize the differential role of macropinocytosis and the actin cytoskeleton on cellular uptake of dextran and cationic cell penetrating peptides octaarginine (R8) and HIV-Tat

M. Al SorajL. HeKaren PeynshaertJ. CousaertDries VercauterenKevin BraeckmansStefaan De SmedtArwin T. Jones

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

2011

Assessing dextran nanogels for intravenous small interfering RNA delivery

Broes NaeyeStefaan De SmedtJo DemeesterKoen Raemdonck

Cellular toxicity of inorganic nanoparticles: common aspects and guidelines for improved nanotoxicity evaluation

Stefaan SoenenPilar Rivera-GilJosé-María MontenegroWolfgang J ParakStefaan De SmedtKevin Braeckmans

A1 Journal Article in NANO TODAY

Dynamic colocalization microscopy to characterize intracellular trafficking of nanomedicines

Dries VercauterenHendrik DeschoutKatrien RemautJohan FJ EngbersenArwyn T JonesJo DemeesterStefaan De SmedtKevin Braeckmans

A1 Journal Article in ACS NANO

Elucidating the mechanisms behind sonoporation with adeno-associated virus-loaded microbubbles

Bart GeersIne LentackerAngelika AlonsoNiek SandersJo DemeesterStephen MeairsStefaan De Smedt

A1 Journal Article in MOLECULAR PHARMACEUTICS

Encapsulation performance of layer-by-layer microcapsules for proteins

Marie-Luce De TemmermanJo DemeesterFilip De VosStefaan De Smedt

A1 Journal Article in BIOMACROMOLECULES

Exploring electrospun fibers for pharmaceutical use

Chaobo HuangStefaan De SmedtJo Demeester

Flotillin-dependent endocytosis and a phagocytosis-like mechanism for cellular internalization of disulfide-based poly(amido amine)/DNA polyplexes

Dries VercauterenMartin PiestLeonardus J van der AAMonerah Al SorajArwyn T JonesJohan FJ EngbersenStefaan De SmedtKevin Braeckmans

A1 Journal Article in BIOMATERIALS

Fluorescence recovery after photobleaching on the confocal laser-scanning microscope: generalized model without restriction on the size of the photobleached disk

Nick SmisdomKevin BraeckmansHendrik DeschoutMartin vandeVenJean-Michel RigoStefaan De SmedtMarcel Ameloot

A1 Journal Article in JOURNAL OF BIOMEDICAL OPTICS

Fluorescence single particle tracking for sizing of nanoparticles in undiluted biological fluids

Kevin BraeckmansKevin BuyensWim Bouquet-GeerardynChris VervaetPhilippe JoyeFilip De VosLaurent PlawinskiLoïc DoeuvreEduardo Angles-CanoNiek Sanders et al.

Gene and cell therapy for cystic fibrosis : from bench to bedside

Massimo ConeseFiorentina AscenzioniA. Christopher BoydCharles CoutelleIda De FinoStefaan De SmedtJoanna RejmanJoseph RoseneckerDirk SchindelhauerBob J. Scholte

A1 Journal Article in JOURNAL OF CYSTIC FIBROSIS

Hemocompatibility of siRNA loaded dextran nanogels

Broes NaeyeHendrik DeschoutMagnus RodingMats RudemoJoris DelangheKatrien DevreeseJo DemeesterKevin BraeckmansStefaan De SmedtKoen Raemdonck

A1 Journal Article in BIOMATERIALS

Lyophilization of protein-loaded polyelectrolyte microcapsules

Marie-Luce De TemmermanJoanna RejmanJohan GrootenThomas De BeerChris VervaetJo DemeesterStefaan De Smedt

A1 Journal Article in PHARMACEUTICAL RESEARCH

Nuclear inclusion of nontargeted and chromatin-targeted polystyrene beads and plasmid DNA containing nanoparticles

Nathalie SymensRudolf WalczakJo DemeesterIain MattajStefaan De SmedtKatrien Remaut

A1 Journal Article in MOLECULAR PHARMACEUTICS

On the cellular processing of nanomedicines for retinal gene therapy and their suitability for intravitreal injections

Dries VercauterenKevin BraeckmansStefaan De Smedt

Particulate vaccines: on the quest for optimal delivery and immune response

Marie-Luce De TemmermanJoanna RejmanJo DemeesterDarrell J IrvineBruno GanderStefaan De Smedt

A1 Journal Article in DRUG DISCOVERY TODAY

Protein macromonomers containing reduction-sensitive linkers for covalent immobilization and glutathione triggered release from dextran hydrogels

Ellen VerheyenSteffen van der WalHendrik DeschoutKevin BraeckmansStefaan De SmedtArjan BarendregtWim E HenninkCornelus F van Nostrum

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Self-assembled liposome-loaded microbubbles: the missing link for safe and efficient ultrasound triggered drug-delivery

Bart GeersIne LentackerNiek SandersJo DemeesterStephen MeairsStefaan De Smedt

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Stimuli-responsive electrospun fibers and their applications

Chaobo HuangStefaan SoenenJoanna RejmanBart LucasKevin BraeckmansJo DemeesterStefaan De Smedt

A1 Journal Article in CHEMICAL SOCIETY REVIEWS

mRNA as gene therapeutic: how to control protein expression

Geertrui TavernierOliwia AndriesJo DemeesterNiek SandersStefaan De SmedtJoanna Rejman

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

mRNA-Lipoplex loaded microbubble contrast agents for ultrasound-assisted transfection of dendritic cells

Marie-Luce De TemmermanHeleen DewitteRoosmarijn VandenbrouckeBart LucasClaude LibertJo DemeesterStefaan De SmedtIne LentackerJoanna Rejman

A1 Journal Article in BIOMATERIALS

2010

Advanced fluorescence microscopy methods illuminate the transfection pathway of nucleic acid nanoparticles

Kevin BraeckmansKevin BuyensBroes NaeyeDries VercauterenHendrik DeschoutKoen RaemdonckKatrien RemautNiek SandersJo DemeesterStefaan De Smedt

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Biodegradable polyelectrolyte microcapsules: antigen delivery tools with Th17 skewing activity after pulmonary delivery

Stefaan De KokerThomas NaessensBruno De GeestPieter BogaertJo DemeesterStefaan De SmedtJohan Grooten

A1 Journal Article in JOURNAL OF IMMUNOLOGY

Colour-coded microcarriers: made to move

Kevin BraeckmansStefaan De Smedt

Design and evaluation of doxorubicin-containing microbubbles for ultrasound-triggered doxorubicin delivery: cytotoxicity and mechanisms involved

Ine LentackerBart GeersJo DemeesterStefaan De SmedtNiek Sanders

A1 Journal Article in MOLECULAR THERAPY

Efficient delivery of intact phosphodiester oligonucleotides by poly-β-amino esters

Katrien RemautNathalie SymensBart LucasJo DemeesterStefaan De Smedt

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Flexible nanosomes (SECosomes) enable efficient siRNA delivery in cultured primary skin cells and in the viable epidermis of ex vivo human skin

Barbara GeusensMireille Van GeleSien BraatStefaan De SmedtMarc CA StuartTarl W ProwWashington SanchezMichael S RobertsNiek SandersJo Lambert

A1 Journal Article in ADVANCED FUNCTIONAL MATERIALS

High intracellular iron oxide nanoparticle concentrations affect cellular cytoskeleton and focal adhesion kinase-mediated signaling

Stefaan SoenenNele NuyttenSimon De MeyerStefaan De SmedtMarcel De Cuyper

A1 Journal Article in SMALL

Impact of chronic pulmonary infection with Pseudomonas aeruginosa on transfection mediated by viral and nonviral vectors

Joanna RejmanIda De FinoMoira ParoniAlessandra BragonziJo DemeesterStefaan De SmedtMassimo Conese

A1 Journal Article in HUMAN GENE THERAPY

Insights in cellular uptake mechanisms of pDNA-polycationic amphiphilic cyclodextrin nanoparticles (CDplexes)

Alejandro Díaz-MoscosoDries VercauterenJoanna RejmanJuan M BenitoCarment Ortiz Mellet MelletStefaan De SmedtJosé M García Fernández

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Interactions of siRNA loaded dextran nanogel with blood cells

Broes NaeyeKoen RaemdonckJo DemeesterStefaan De Smedt

Monitoring the disassembly of siRNA polyplexes in serum is crucial for predicting their biological efficacy

Kevin BuyensMartin MeyerErnst WagnerJo DemeesterStefaan De SmedtNiek Sanders

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Optimizing siRNA-nanocarriers for systemic delivery by monitoring their behavior in blood

Kevin BuyensStefaan De SmedtNiek Sanders

PEGylation of biodegradable dextran nanogels for siRNA delivery

Broes NaeyeKoen RaemdonckKatrien RemautB SproatJo DemeesterStefaan De Smedt

A1 Journal Article in EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES

Photopolymerized thermosensitive poly(HPMAlactate)-PEG-based hydrogels : effect of network design on mechanical properties, degradation, and release behavior

Roberta CensiTina VermondenHendrik DeschoutKevin BraeckmansPiera di MartinoStefaan De SmedtCornelus F van NostrumWim E Hennink

A1 Journal Article in BIOMACROMOLECULES

Polyelectrolyte capsules-containing HIV-1 p24 and poly I:C modulate dendritic cells to stimulate HIV-1-specific immune responses

Winni De HaesStefaan De KokerCharlotte PollardDerek AtkinsonErika VliegheJessy HosteJoanna RejmanStefaan De SmedtJohan GrootenGuido Vanham et al.

A1 Journal Article in MOLECULAR THERAPY

Prolonged gene silencing by combining siRNA nanogels and photochemical internalization

Koen RaemdonckBroes NaeyeA HogsetJo DemeesterStefaan De Smedt

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Pulmonary delivery of mRNA: in vitro and in vivo evaluation

Oliwia AndriesJoanna RejmanCindy PelemanTony LahoutteStefaan De SmedtJo DemeesterNiek Sanders

Self-exploding capsules

Bruno De GeestStefaan De KokerJo DemeesterStefaan De SmedtWim E Hennink

A1 Journal Article in POLYMER CHEMISTRY

Single particle tracking

Kevin BraeckmansDries VercauterenJo DemeesterStefaan De Smedt

Bookchapter in Nanoscopy and multidimensional optical fluorescence microscopy

Sizing nanomatter in biological fluids by fluorescence single particle tracking

Kevin BraeckmansKevin BuyensWim Bouquet-GeerardynChris VervaetPhilippe JoyeFilip De VosLaurent PlawinskiLoic DoeuvreEduardo Angles-CanoNiek Sanders et al.

A1 Journal Article in NANO LETTERS

The use of inhibitors to study endocytic pathways of gene carriers: optimization and pitfalls

Dries VercauterenRoosmarijn VandenbrouckeArwyn T JonesJoanna RejmanJo DemeesterStefaan De SmedtNiek SandersKevin Braeckmans

A1 Journal Article in MOLECULAR THERAPY

Transport and delivery of antimicrobial agents in Burkholderia biofilms

Katrien ForierAnne-Sophie MessiaenTom CoenyeHans NelisShari CelenSerge Van CalenberghStefaan De SmedtJo DemeesterKevin Braeckmans

Unbreakable codes in electrospun fibers : digitally encoded polymers to stop medicine counterfeiting

Chaobo HuangBart LucasChris VervaetKevin BraeckmansSerge Van CalenberghIzet KaralicMado VandewoestyneDieter DeforceJo DemeesterStefaan De Smedt

A1 Journal Article in ADVANCED MATERIALS

Unbreakable codes in electrospun fibers to stop medicine counterfeiting

Chaobo HuangBart LucasChris VervaetKevin BraeckmansSerge Van CalenberghIzet KaralicMado VandewoestyneDieter DeforceJo DemeesterStefaan De Smedt

Vaccination of turkeys against Chlamydophila psittaci through optimised DNA formulation and administration

Kristel VerminnenDelphine Sylvie Anne BeeckmanNiek SandersStefaan De SmedtDaisy Vanrompay

A1 Journal Article in VACCINE

mRNA transfection of cervical carcinoma and mesenchymal stem cells mediated by cationic carriers

Joanna RejmanGeertrui TavernierNeda BavarsadJoseph DemeesterStefaan De Smedt

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

siRNA containing nanoparticles: stability of encapsulation and particle size

Kevin BuyensKevin BraeckmansJo DemeesterStefaan De SmedtNiek Sanders

2009

Advanced nanogel engineering for drug delivery

Koen RaemdonckJo DemeesterStefaan De Smedt

A1 Journal Article in Soft Matter

Biodegradable Dextran Nanogels for RNA Interference: Focusing on Endosomal Escape and Intracellular siRNA Delivery

Koen RaemdonckBroes NaeyeKevin BuyensRoosmarijn VandenbrouckeA HogsetJo DemeesterStefaan De Smedt

A1 Journal Article in Advanced Functional Materials

Biodegradable dextran nanogels as functional carriers for the intracellular delivery of small interfering RNA

Koen RaemdonckStefaan De SmedtJo Demeester

Cationic polymers to optimize aerosol delivery of a Chlamydophila psittaci DNA vaccine

Kristel VerminnenNiek SandersStefaan De SmedtDaisy Vanrompay

Design and evaluation of drug loaded microbubbles for ultrasound guided cancer therapy

Ine LentackerNiek SandersStefaan De SmedtJo Demeester

Development of a sensitive diagnostic multiplex platform based on digitally encoded microcarriers

Stefaan DerveauxJo DemeesterStefaan De Smedt

Drug loaded microbubble design for ultrasound triggered delivery

Ine LentackerStefaan De SmedtNiek Sanders

A1 Journal Article in SOFT MATTER

Elucidating the Encapsulation of Short Interfering RNA in PEGylated Cationic Liposomes

Kevin BuyensJo DemeesterStefaan De SmedtNiek Sanders

A1 Journal Article in Langmuir

Evaluation of ethosomes for delivery of siRNA into cultured primary melanocytes and skin

Barbara GeusensSien BraatM StuartStefaan De SmedtNiek SandersT ProwM RobertsJo LambertMireille Van Gele

Extracellular barriers in respiratory gene therapy

Niek SandersCarsten RudolphKevin BraeckmansStefaan De SmedtJo Demeester

A1 Journal Article in ADVANCED DRUG DELIVERY REVIEWS

Photopolymerized thermosensitive hydrogels for tailorable diffusion-controlled protein delivery

R CensiT VermondenMJ van SteenbergenHendrik DeschoutKevin BraeckmansStefaan De SmedtCF van NostrumP di MartinoWE Hennink

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Polyelectrolyte Microcapsules as Antigen Delivery Vehicles To Dendritic Cells: Uptake, Processing, and Cross-Presentation of Encapsulated Antigens

Stefaan De KokerBruno De GeestSatwinder SinghRiet De RyckeThomas NaessensYvette Van KooykJo DemeesterStefaan De SmedtJohan Grooten

A1 Journal Article in ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Polyelectrolyte microcapsules for biomedical applications

Bruno De GeestStefaan De KokerGB SukhorukovO KreftWJ ParakAndre SkirtachJo DemeesterStefaan De SmedtWim E Hennink

A1 Journal Article in Soft Matter

Protein-Release Behavior of Self-Assembled PEG-beta-Cyclodextrin/PEG-Cholesterol Hydrogels

F van de ManakkerKevin BraeckmansN el MorabitStefaan De SmedtCF van NostrumWE Hennink

A1 Journal Article in ADVANCED FUNCTIONAL MATERIALS

Pulsed in vitro release and in vivo behavior of exploding microcapsules

Bruno De GeestStefaan De KokerJo DemeesterStefaan De SmedtWE Hennink

A1 Journal Article in Journal of Controlled Release

Stability of siRNA polyplexes from poly(ethylenimine) and poly(ethylenimine)-g-poly(ethylene glycol) under in vivo conditions: Effects on pharmacokinetics and biodistribution measured by Fluorescence Fluctuation Spectroscopy and Single Photon Emission Computed Tomography (SPECT) imaging

OM MerkelD LibrizziA PfestroffT SchurratKevin BuyensNiek SandersStefaan De SmedtM BeheT Kissel

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Stable long-term intracellular labelling with fluorescently tagged cationic magnetoliposomes

Stefaan JH SoenenDries VercauterenKevin BraeckmansWim NoppeStefaan De SmedtMarcel De Cuyper

A1 Journal Article in CHEMBIOCHEM

The role of nanoparticle concentration-dependent induction of cellular stress in the internalization of non-toxic cationic magnetoliposomes

Stefaan SoenenEszter IllyesDries VercauterenKevin BraeckmansZsuzsa MajerStefaan De SmedtMarcel De Cuyper

A1 Journal Article in BIOMATERIALS

Ultradeformable cationic liposomes for delivery of small interfering RNA (siRNA) into human primary melanocytes

Barbara GeusensJo LambertStefaan De SmedtKevin BuyensNiek SandersMireille Van Gele

A1 Journal Article in Journal of Controlled Release

Ultrasound exposure of lipoplex loaded microbubbles facilitates direct cytoplasmic entry of the lipoplexes

Ine LentackerNan WangRoosmarijn VandenbrouckeJo DemeesterStefaan De SmedtNiek Sanders

A1 Journal Article in MOLECULAR PHARMACEUTICS

2008

A fast and sensitive method for measuring the integrity of siRNA-carrier complexes in full human serum

Kevin BuyensBart LucasKoen RaemdonckKevin BraeckmansJ VERCAMMENJ HENDRIXY ENGELBORGHSStefaan De SmedtNiek Sanders

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

A new FRAP/FRAPa method for three-dimensional diffusion measurements based on multiphoton excitation microscopy

Davide MazzaKevin BraeckmansFrancesca CellaIlaria TestaDries VercauterenJo DemeesterStefaan De SmedtAlberto Diaspro

A1 Journal Article in BIOPHYSICAL JOURNAL

Advanced fluorescence microscopy methods illuminate the transfection pathway

Stefaan De SmedtKevin BraeckmansDries VercauterenJo Demeester

Biodegradable microcapsules designed via "click" chemistry

Bruno De GeestWim Van CampFilip Du PrezStefaan De SmedtJo DemeesterW HENNINK

Biodegradable microcapsules designed via 'click' chemistry

Bruno De GeestWim Van CampFilip Du PrezStefaan De SmedtJo DemeesterWE HENNINK

A1 Journal Article in CHEMICAL COMMUNICATIONS

Biodegradable microgels by click chemistry

Bruno De GeestWim Van CampFilip Du PrezJo DemeesterStefaan De SmedtWim E Hennink

Biodegradable microgels by click chemistry

Bruno De GeestWim Van CampFilip Du PrezJo DemeesterStefaan De SmedtW HENNINK

Biodegradable polymers as non-viral carriers for plasmid DNA delivery

J LUTENCF VAN NOSTRUINStefaan De SmedtWE HENNINK

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Can ultrasound solve the transport barrier of the neural retina?

Liesbeth PeetersIne LentackerRoosmarijn VandenbrouckeBart LucasJo DemeesterNiek SandersStefaan De Smedt

A1 Journal Article in PHARMACEUTICAL RESEARCH

Cationic polymers to optimize aerosol delivery of a Chlamydophila psittaci DNA vaccine

Kristel VerminnenNiek SandersStefaan De SmedtDaisy Vanrompay

Cationic polymers to optimize aerosol delivery of a Chlamydophila psittaci DNA vaccine

Kristel VerminnenNiek SandersStefaan De SmedtDaisy Vanrompay

Circumventing the endocytotic uptake of highly PEGylated lipoplexes by using lipoplex loaded microbubbles and ultrasound can drastically increase their transfection efficiency

Ine LentackerStefaan De SmedtNiek Sanders

Degradable microgels and hollow capsules by 'click' chemistry

Bruno De GeestWim Van CampFilip Du PrezJo DemeesterStefaan De SmedtW Hennink

Degradable multilayer films and hollow capsules via a 'Click' strategy

Bruno De GeestWim Van CampFilip Du PrezStefaan De SmedtJo DemeesterWE HENNINK

A1 Journal Article in MACROMOLECULAR RAPID COMMUNICATIONS

Dextran microgels for time-controlled delivery of siRNA

Koen RaemdonckTinneke Van ThienenRoosmarijn VandenbrouckeNiek SandersJo DemeesterStefaan De Smedt

A1 Journal Article in ADVANCED FUNCTIONAL MATERIALS

Effect of particle size and charge on the network properties of microsphere-based hydrogels

SR VAN TOMMECF VAN NOSTRUMM DIJKSTRAStefaan De SmedtWE HENNINK

A1 Journal Article in EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS

Evaluation of Digitally Encoded Layer-by-layer Coated Microparticles as Cell Carriers

Farzaneh FayazpourBart LucasRoosmarijn VandenbrouckeStefaan DerveauxJan TavernierSam LievensJo DemeesterStefaan De Smedt

A1 Journal Article in ADVANCED FUNCTIONAL MATERIALS

Evaluation of encoded layer-by-layer coated microparticles as protease sensors

Barbara StubbeKris GevaertStefaan DerveauxKevin BraeckmansBruno De GeestMarc GoethalsJoël VandekerckhoveJo DemeesterStefaan De Smedt

A1 Journal Article in ADVANCED FUNCTIONAL MATERIALS

Layer-by-layer coated digitally encoded microcarriers for quantification of proteins in serum and plasma

Stefaan DerveauxBarbara StubbeC ROELANTM LEBLANSBruno De GeestJo DemeesterStefaan De Smedt

A1 Journal Article in ANALYTICAL CHEMISTRY

Loading of PEGylated siRNA lipoplexes on microbubbles restores their siRNA delivery capacity in the presence of ultrasound

Roosmarijn VandenbrouckeIne LentackerJo DemeesterStefaan De SmedtNiek Sanders

Maintaining the silence: reflections on long-term RNAi

Koen RaemdonckRoosmarijn VandenbrouckeJo DemeesterNiek SandersStefaan De Smedt

A1 Journal Article in DRUG DISCOVERY TODAY

Microcapsules Ejecting Nanosized Species into the Environment

Bruno De GeestMJ MCSHANEJo DemeesterStefaan De SmedtWE HENNINK

A1 Journal Article in JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

New strategies for nucleic acid delivery to conquer cellular and nuclear membranes

Ine LentackerRoosmarijn VandenbrouckeBart LucasJo DemeesterStefaan De SmedtNiek Sanders

A1 Journal Article in Journal of Controlled Release

Opportunities for digitally encoded microcarriers in pharmacy and cell-based assays

Farzaneh FayazpourBart LucasN HUYGEBAERTKevin BraeckmansStefaan DerveauxRoosmarijn VandenbrouckeJean Paul RemonJo DemeesterChris VervaetStefaan De Smedt

Prolonged gene silencing in hepatoma cells and primary hepatocytes after siRNA delivery with biodegradable poly(beta-amino esters)

Roosmarijn VandenbrouckeBruno De GeestJo DemeesterStefaan De SmedtNiek Sanders

Prolonged gene silencing in hepatoma cells and primary hepatocytes after small interfering RNA delivery with biodegradable poly(beta-amino esters)

Roosmarijn VandenbrouckeBruno De GeestStefan BonnéM VINKENT VAN HAECKEH HEIMBERGE WAGNERV ROGIERSStefaan De SmedtJo Demeester et al.

A1 Journal Article in JOURNAL OF GENE MEDICINE

Screening poly(ethyleneglycol) micro- and nanogels for drug delivery purposes

Tinneke Van ThienenJo DemeesterStefaan De Smedt

A1 Journal Article in INTERNATIONAL JOURNAL OF PHARMACEUTICS

Self exploding microcapsules

Bruno De GeestStefaan De KokerJo DemeesterStefaan De SmedtWim E Hennink

Self-Exploding Beads Releasing Microcarriers

Bruno De GeestStefaan De KokerKristof ImmesoeteJo DemeesterStefaan De SmedtWE HENNINK

A1 Journal Article in ADVANCED MATERIALS

Stimuli-responsive polyelectrolyte microcapsules for biomedical applications

Bruno De GeestA SkirtachG SukhorukovJo DemeesterStefaan De SmedtWE Hennink

Synergism between particle-based multiplexing and microfluidics technologies may bring diagnostics closer to the patient

Stefaan DerveauxBarbara StubbeKevin BraeckmansC ROELANTK SATOJo DemeesterStefaan De Smedt

A1 Journal Article in ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Ultrasound assisted siRNA delivery using PEG-siPlex loaded microbubbles

Roosmarijn VandenbrouckeIne LentackerJo DemeesterStefaan De SmedtNiek Sanders

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

2007

Assessment of fluorescently labelled polyelectrolyte distribution in sludge flocs by confocal laser scanning microscopy

Daan CurversHans SaveynBart LucasStefaan De SmedtLies BonamiFilip Du PrezPatric Van OostveldtPaul Van der Meeren

Can we better understand the intracellular behavior of DNA nanoparticles by fluorescence correlation spectroscopy?

Katrien RemautBart LucasKoen RaemdonckKevin BraeckmansJo DemeesterStefaan De Smedt

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Cell uptake, cytoplasmic diffusion and nuclear access of a 6.5 nm diameter dendrimer

P RUENRAROENGSAKKT AL-JAMALN HARTELLKevin BraeckmansStefaan De SmedtAT FLORENCE

A1 Journal Article in INTERNATIONAL JOURNAL OF PHARMACEUTICS

Cellular entry pathway and gene transfer capacity of TAT-modified lipoplexes

Roosmarijn VandenbrouckeStefaan De SmedtJo DemeesterNiek Sanders

A1 Journal Article in BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Challenges in non-viral ocular gene transfer

Liesbeth PeetersNiek SandersJo DemeesterStefaan De Smedt

A1 Journal Article in BIOCHEMICAL SOCIETY TRANSACTIONS

Digitally encoded drug tablets to combat counterfeiting

Farzaneh FayazpourBart LucasN HUYGHEBAERTKevin BraeckmansStefaan DerveauxBG STUBBEJean Paul RemonJo DemeesterChris VervaetStefaan De Smedt

A1 Journal Article in ADVANCED MATERIALS

In vivo cellular uptake, degradation, and biocompatibility of polyelectrolyte microcapsules

Stefaan De KokerBruno De GeestClaude CuvelierLiesbeth FerdinandeWies DeckersWE HENNINKStefaan De SmedtNico Mertens

A1 Journal Article in ADVANCED FUNCTIONAL MATERIALS

Influence of free chains on the swelling pressure of PEG-HEMA and dex-HEMA hydrogels

Tinneke Van ThienenF HORKAYKevin BraeckmansBG STUBBEJo DemeesterStefaan De Smedt

A1 Journal Article in INTERNATIONAL JOURNAL OF PHARMACEUTICS

Line FRAP with the confocal laser scanning microscope for diffusion measurements in small regions of 3-D samples

Kevin BraeckmansKatrien RemautRoosmarijn VandenbrouckeBart LucasStefaan De SmedtJo Demeester

A1 Journal Article in BIOPHYSICAL JOURNAL

Lipoplex-loaded microbubbles for gene delivery: A Trojan horse controlled by ultrasound

Ine LentackerStefaan De SmedtJo DemeesterVeerle Van MarckMarc BrackeNiek Sanders

A1 Journal Article in ADVANCED FUNCTIONAL MATERIALS

Mucolytic activity of bacterial and human chitinases

Niek SandersVGH EIJSLNKPS VAN DEN PANGAARTRJJ VAN NEERVENPJ SIMONSStefaan De SmedtJo Demeester

A1 Journal Article in BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS

Multifunctional layer-by-layer coating of digitally encoded microparticles

Stefaan DerveauxBruno De GeestC ROELANTKevin BraeckmansJo DemeesterStefaan De Smedt

A1 Journal Article in LANGMUIR

Nuclear accumulation of plasmid DNA can be enhanced by non-selective gating of the nuclear pore

Roosmarijn VandenbrouckeBart LucasJo DemeesterStefaan De SmedtNiek Sanders

Nuclear accumulation of plasmid DNA can be enhanced by non-selective gating of the nuclear pore

Roosmarijn VandenbrouckeBart LucasJo DemeesterStefaan De SmedtNiek Sanders

A1 Journal Article in NUCLEIC ACIDS RESEARCH

Nucleic acid delivery: Where material sciences and bio-sciences meet

Katrien RemautNiek SandersBruno De GeestKevin BraeckmansJo DemeesterStefaan De Smedt

A1 Journal Article in MATERIALS SCIENCE & ENGINEERING R-REPORTS

Pegylation of liposomes favours the endosornal degradation of the delivered phosphodiester oligonucleotides

Katrien RemautBart LucasKevin BraeckmansJo DemeesterStefaan De Smedt

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Post-pegylated lipoplexes are promising vehicles for gene delivery in RPE cells

Liesbeth PeetersNiek SandersA JONESJo DemeesterStefaan De Smedt

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Protection of oligonucleotides against enzymatic degradation by pegylated and nonpegylated branched polyethyleneimine

Katrien RemautBart LucasKoen RaemdonckKevin BraeckmansJo DemeesterStefaan De Smedt

A1 Journal Article in BIOMACROMOLECULES

Protein release from biodegradable dextran nanogels

Tinneke Van ThienenKoen RaemdonckJo DemeesterStefaan De Smedt

A1 Journal Article in LANGMUIR

Release mechanisms for polyelectrolyte capsules

Bruno De GeestNiek SandersGB SUKHORUKOVJo DemeesterStefaan De Smedt

A1 Journal Article in CHEMICAL SOCIETY REVIEWS

Self-rupturing and hollow microcapsules prepared from bio-polyelectrolyte-coated microgels

Bruno De GeestC DEJUGNATM PREVOTGB SUKHORUKOVJo DemeesterStefaan De Smedt

A1 Journal Article in ADVANCED FUNCTIONAL MATERIALS

Sensitive spectroscopic detection of large and denatured protein aggregates in solution by use of the fluorescent dye Nile red

M SUTTERS OLIVEIRANiek SandersBart LucasA VAN HOEKMA HINKAJWG VISSERStefaan De SmedtWE HENNINKW JISKOOT

A1 Journal Article in JOURNAL OF FLUORESCENCE

Stimuli-responsive multilayered hybrid nanoparticle/polyelectrolyte capsules

Bruno De GeestAndre SkirtachThomas De BeerGB SUKHORUKOVLieven BrackeWilly BaeyensJo DemeesterStefaan De Smedt

A1 Journal Article in MACROMOLECULAR RAPID COMMUNICATIONS

Ultrasound-triggered release from multilayered capsules

Bruno De GeestAndre SkirtachAA MAMEDOVAA ANTIPOVNA KOTOVStefaan De SmedtGB SUKHORUKOV

A1 Journal Article in SMALL

Wanted and unwanted properties of surface PEGylated nucleic acid nanoparticles in ocular gene transfer

Niek SandersLiesbeth PeetersIne LentackerJo DemeesterStefaan De Smedt

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

2006

Anomalous photobleaching in fluorescence recovery after photobleaching measurements due to excitation saturation- a case study for fluorescein

Kevin BraeckmansBarbara StubbeKatrien RemautJo DemeesterStefaan De Smedt

A1 Journal Article in JOURNAL OF BIOMEDICAL OPTICS

Characterization of diffusion of macromolecules in konjac glucomannan solutions and gels by fluorescence recovery after photobleaching technique

F ALVAREZ-MANCENIDOKevin BraeckmansStefaan De SmedtJo DemeesterM LANDINR MARTINEZ-PACHECO

A1 Journal Article in INTERNATIONAL JOURNAL OF PHARMACEUTICS

Characterization of the mode of incorporation of lipophilic compounds in solid dispersions at the nanoscale using fluorescence resonance energy transfer (FRET)

DJ VAN DROOGEKevin BraeckmansWLJ HINRICHSKatrien RemautStefaan De SmedtHW FRIJLINK

A1 Journal Article in MACROMOLECULAR RAPID COMMUNICATIONS

Degradation behavior of dextran hydrogels composed of positively and negatively charged microspheres

SR VAN TOMMECF VAN NOSTRUMStefaan De SmedtWE HENNINK

A1 Journal Article in BIOMATERIALS

Delivery of phosphodiester oligonucleotides: Can DOTAP/DOPE liposomes do the trick?

Katrien RemautBart LucasKevin BraeckmansNiek SandersJo DemeesterStefaan De Smedt

A1 Journal Article in BIOCHEMISTRY

Glucose-responsive polyelectrolyte capsules

Bruno De GeestAM JONASJo DemeesterStefaan De Smedt

A1 Journal Article in LANGMUIR

In situ analysis of single-stranded and duplex siRNA integrity in living cells

Koen RaemdonckKatrien RemautBart LucasNiek SandersJo DemeesterStefaan De Smedt

A1 Journal Article in BIOCHEMISTRY

Influence of plasmid DNA topology on the transfection properties of DOTAP/DOPE lipoplexes

Katrien RemautNiek SandersFarzaneh FayazpourJo DemeesterStefaan De Smedt

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Intracellularly degradable polyelectrolyte microcapsules

Bruno De GeestRoosmarijn VandenbrouckeAM GUENTHERGB SUKHORUKOVWE HENNINKNiek SandersJo DemeesterStefaan De Smedt

A1 Journal Article in ADVANCED MATERIALS

Layer-by-layer coating of degradable microgels for pulsed drug delivery

Bruno De GeestC DEJUGNATEllen VerhoevenGB SUKHORUKOVAM JONASJ PLAINJo DemeesterStefaan De Smedt

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Microbubbles which bind and protect DNA against nucleases

Ine LentackerStefaan De SmedtJo DemeesterNiek Sanders

Physicochemical and transfection properties of cationic hydroxyethylcellulose/DNA nanoparticles

Farzaneh FayazpourBart LucasC ALVAREZ-LORENZONiek SandersJo DemeesterStefaan De Smedt

A1 Journal Article in BIOMACROMOLECULES

Pulsed drug delivery

Bruno De GeestEls MehuysG LaekemanJo DemeesterStefaan De Smedt

A2 Journal Article in Expert Opinion on Drug Delivery

Role of magnesium in the failure of rhDNase therapy in patients with cystic fibrosis

Niek SandersH FRANCKXK DE BOECKJurgen HaustraeteStefaan De SmedtJo Demeester

A1 Journal Article in THORAX

Self-exploding lipid-coated microgels

Bruno De GeestBarbara StubbeAM JONASTinneke Van ThienenWLJ HINRICHSJo DemeesterStefaan De Smedt

A1 Journal Article in BIOMACROMOLECULES

The choice of a suitable oligosaccharide to prevent aggregation of PEGylated nanoparticles during freeze thawing and freeze drying

WLJ HINRICHSFA MANCENIDONiek SandersKevin BraeckmansStefaan De SmedtJo DemeesterHW FRIJLINK

A1 Journal Article in INTERNATIONAL JOURNAL OF PHARMACEUTICS

The internalization route resulting in successful gene expression depends on polyethylenimine both cell line and polyplex type

K VON GERSDORFFNiek SandersRoosmarijn VandenbrouckeStefaan De SmedtE WAGNERM OGRIS

A1 Journal Article in MOLECULAR THERAPY

Ultrasound-responsive polymer-coated microbubbles that bind and protect DNA

Ine LentackerBruno De GeestRoosmarijn VandenbrouckeLiesbeth PeetersJo DemeesterStefaan De SmedtNiek Sanders

A1 Journal Article in LANGMUIR

2005

FRET-FCS as a tool to evaluate the stability of oligonucleotide drugs after intracellular delivery

Katrien RemautBart LucasKevin BraeckmansNiek SandersStefaan De SmedtJo Demeester

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Fluorescence energy transfer to study the degradation of double-labeled oligonucleotides

Katrien RemautBart LucasKevin BraeckmansNiek SandersStefaan De SmedtJo Demeester

Inulin is a promising cryo- and lyoprotectant for PEGylated lipoplexes

WLJ HINRICHSNiek SandersStefaan De SmedtJo DemeesterHW FRIJLINK

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Mobility of model proteins in hydrogels composed of oppositely charged dextran microspheres studied by protein release and fluorescence recovery after photobleaching

SR VAN TOMMEBruno De GeestKevin BraeckmansStefaan De SmedtF SIEPMANNJ SIEPMANNCF VAN NOSTRUMWE HENNINK

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

On the synthesis and characterization of biodegradable dextran nanogels with tunable degradation properties

Tinneke Van ThienenBart LucasFM FLESCHCF VAN NOSTRUMJo DemeesterStefaan De Smedt

A1 Journal Article in MACROMOLECULES

Protection of oligonucleotides against nucleases by pegylated and non-pegylated liposomes as studied by fluorescence correlation spectroscopy

Katrien RemautBart LucasKevin BraeckmansNiek SandersJo DemeesterStefaan De Smedt

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Self-gelling hydrogels based on oppositely charged dextran microspheres

SR VAN TOMMEMJ VAN STEENBERGENStefaan De SmedtCF VAN NOSTRUMWE HENNINK

A1 Journal Article in BIOMATERIALS

Self-rupturing microcapsules

Bruno De GeestC DEJUGNATGB SUKHORUKOVKevin BraeckmansStefaan De SmedtJo Demeester

A1 Journal Article in ADVANCED MATERIALS

Studying biophysical barriers to DNA delivery by advanced light microscopy

Stefaan De SmedtKatrien RemautBart LucasKevin BraeckmansNiek SandersJo Demeester

A1 Journal Article in ADVANCED DRUG DELIVERY REVIEWS

Studying the intracellular dissociation of polymer-oligonucleotide complexes by dual color fluorescence fluctuation spectroscopy and confocal imaging

Bart LucasKatrien RemautNiek SandersKevin BraeckmansStefaan De SmedtJo Demeester

A1 Journal Article in BIOCHEMISTRY

Synthesis of monodisperse biodegradable microgels in microfluidic devices

Bruno De GeestJP URBANSKIT THORSENJo DemeesterStefaan De Smedt

A1 Journal Article in LANGMUIR

Towards a better understanding of the dissociation behavior of liposome-oligonucleotide complexes in the cytosol of cells

Bart LucasKatrien RemautNiek SandersKevin BraeckmansStefaan De SmedtJo Demeester

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Vitreous: a barrier to nonviral ocular gene therapy

Liesbeth PeetersNiek SandersKevin BraeckmansKoen BousseryJohan Van de VoordeStefaan De SmedtJo Demeester

A1 Journal Article in INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE

2004

"Programmed polymeric devices" for pulsed drug delivery

Barbara StubbeStefaan De SmedtJo Demeester

A1 Journal Article in PHARMACEUTICAL RESEARCH

Enhancement of nuclear uptake of therapeutic genes and macromolecules by using NLS and PTD containing viral peptides

Roosmarijn VandenbrouckeEls VanderleydenStefaan De SmedtNiek SandersJo Demeester

Modeling the swelling pressure of degrading hydroxyethylmethacrylate-grafted dextran hydrogels

BG AMSDENBG STUBBEF HORKAYStefaan De SmedtJo Demeester

A1 Journal Article in JOURNAL OF POLYMER SCIENCE PART B-POLYMER PHYSICS

On the biological activity of anti-ICAM-1 oligonucleotides complexed to non-viral carriers

Bart LucasElsa Van RompaeyKatrien RemautNiek SandersStefaan De SmedtJo Demeester

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Studying pegylated DNA complexes by dual color fluorescence fluctuation spectroscopy

Bart LucasKatrien RemautKevin BraeckmansJurgen HaustraeteStefaan De SmedtJo Demeester

A1 Journal Article in MACROMOLECULES

Swelling pressure of hydrogels that degrade through different mechanisms

Barbara StubbeWE HENNINKStefaan De SmedtJ DEMEESTERL

A1 Journal Article in MACROMOLECULES

2003

Encoding microcarriers by spatial selective photobleaching

Kevin BraeckmansStefaan De SmedtC ROELANTM LEBLANSRomain PauwelsJo Demeester

A1 Journal Article in NATURE MATERIALS

Mobility and stability of gene complexes in biogels

Niek SandersStefaan De SmedtJo Demeester

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Tailoring the swelling pressure of degrading dextran hydroxyethyl methacrylate hydrogels

Barbara StubbeF HORKAYB AMSDENWE HENNINKStefaan De SmedtJo Demeester

A1 Journal Article in BIOMACROMOLECULES

Three-dimensional fluorescence recovery after photobleaching with the confocal scanning laser microscope

Kevin BraeckmansLiesbeth PeetersNiek SandersStefaan De SmedtJo Demeester

A1 Journal Article in BIOPHYSICAL JOURNAL

2002

A comparison between the use of dynamic mechanical analysis and oscillatory shear rheometry for the characterisation of hydrogels.

T MEYVISBarbara StubbeMJ VAN STEENBERGENWE HENNINKStefaan De SmedtJo Demeester

A1 Journal Article in INTERNATIONAL JOURNAL OF PHARMACEUTICS

Dual-color fluorescence fluctuation spectroscopy to study the complexation between poly-l-lysine and oligonucleotides

Bart LucasElsa Van RompaeyStefaan De SmedtJo DemeesterPatric Van Oostveldt

A1 Journal Article in MACROMOLECULES

Encoding microcarriers : present and future technologies

Kevin BraeckmansStefaan De SmedtMarc LeblansRomain PauwelsJo Demeester

A1 Journal Article in NATURE REVIEWS DRUG DISCOVERY

In vivo evaluation of matrix pellets containing nanocrystalline ketoprofen

Geert VERGOTEChris VervaetIsabel Van DriesscheSerge HosteStefaan De SmedtJo DemeesterRA JAINS RUDDYJean Paul Remon

A1 Journal Article in INTERNATIONAL JOURNAL OF PHARMACEUTICS

On the transport of lipoplexes through cystic fibrosis sputum.

Niek SandersElsa Van RompaeyStefaan De SmedtJo Demeester

A1 Journal Article in PHARMACEUTICAL RESEARCH

Pegylated GL67 lipoplexes retain their gene transfection activity after exposure to components of CF mucus.

Niek SandersStefaan De SmedtSH CHENGJo Demeester

A1 Journal Article in GENE THERAPY

Swelling pressure observations on degrading dex-HEMA hydrogels.

Barbara StubbeKevin BraeckmansF HORKAYWE HENNINKStefaan De SmedtJo Demeester

A1 Journal Article in MACROMOLECULES

2001

An oral controlled release matrix pellet formulation containing nanocrystalline ketoprofen

Geert VERGOTEChris VervaetIsabel Van DriesscheSerge HosteStefaan De SmedtJo DemeesterRA JAINS RUDDYJean Paul Remon

A1 Journal Article in INTERNATIONAL JOURNAL OF PHARMACEUTICS

Biodegradable hydrogels based on stereocomplex formation between lactic acid oligomers grafted to dextran.

SJ DE JONGStefaan De SmedtJo DemeesterCF VAN NOSTRUMJJ KETTENES-VAN DEN BOSCHWE HENNINK

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Fluorescence fluctuation analysis for the study of interactions between oligonucleotides and polycationic polymers.

Elsa Van RompaeyY CHENJD MULLERE GRATTONE VAN CRAENENBROECKY ENGELBORGHSStefaan De SmedtJo Demeester

A1 Journal Article in BIOLOGICAL CHEMISTRY

Interactions between oligonucleotides and cationic polymers investigated by fluorescence correlation spectroscopy.

Elsa Van RompaeyY ENGELBORGHSNiek SandersStefaan De SmedtJo Demeester

A1 Journal Article in PHARMACEUTICAL RESEARCH

On the release of proteins from degrading dextran methacrylate hydrogels and the correlation with the rheologic properties of the hydrogels.

T MEYVISStefaan De SmedtBarbara StubbeW HENNINKJo Demeester

A1 Journal Article in PHARMACEUTICAL RESEARCH

Structural alterations of gene complexes by cystic fibrosis sputum.

Niek SandersElsa Van RompaeyStefaan De SmedtJo Demeester

A1 Journal Article in AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE

The in vitro evaluation of 'azo containing polysaccharide gels' for colon delivery.

Barbara StubbeB MARISG VAN DEN MOOTERStefaan De SmedtJo Demeester

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

2000

Cationic Polymer Based Gene Delivery Systems.

Stefaan De SmedtJo DemeesterWE HENNINK

A1 Journal Article in PHARMACEUTICAL RESEARCH

Combined gelatin-chondroitin sulfate hydrogels for controlled release of cationic antibacterial proteins.

AJ KUIJPERSGHM ENGBERSTKL MEYVISStefaan De SmedtJo DemeesterJ KRIJGSVELDSAJ ZAATJ DANKERTJ FEIJEN

A1 Journal Article in MACROMOLECULES

Complex formation between cationic polymethacrylates and oligonucleotides.

Elsa Van RompaeyNiek SandersStefaan De SmedtJo DemeesterE VAN CRAENENBROECKY ENGELBORGHS

A1 Journal Article in MACROMOLECULES

Cystic fibrosis sputum. A barrier to the transport of nanospheres.

Niek SandersStefaan De SmedtElsa Van RompaeyPaul SimoensFrans De BaetsJo Demeester

A1 Journal Article in AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE

Influence of the degradation mechanism of hydrogels on their elastic and swelling properties during degradation.

T MEYVISStefaan De SmedtJo DemeesterWE HENNINK

A1 Journal Article in MACROMOLECULES

Novel self-assembled hydrogels by stereocomplex formation in aqueous solution of enantiomeric lactic acid oligomers grafted to dextran.

SJ DE JONGStefaan De SmedtM WAHLSJo DemeesterJJ KETTENES-VAN DEN BOSCHWE HENNINK

A1 Journal Article in MACROMOLECULES

Optimisation of an in vitro procedure for the determination of the enzymatic inhibition potency of multifunctional polymers.

Dieter AmeyeJody VoorspoelsJean Paul RemonJo DemeesterStefaan De Smedt

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Pore sizes in hydrated dextran microspheres.

RJH STENEKESStefaan De SmedtJo DemeesterGZ SUNZB ZHANGWE HENNINK

A1 Journal Article in BIOMACROMOLECULES

The physical properties of biogels and their permeability for macromolecular drugs and colloidal drug carriers.

Niek SandersStefaan De SmedtJo Demeester

A1 Journal Article in JOURNAL OF PHARMACEUTICAL SCIENCES

1999

A new microphotolysis based approach for mapping the mobility of drugs in microscopic drug delivery devices

Stefaan De SmedtTom KL MeyvisPatric Van OostveldtJo Demeester

A1 Journal Article in PHARMACEUTICAL RESEARCH

Characterization of the network structure of carbodiimide cross-linked gelatin gels.

AJ KUIJPERSG ENGBERSJ FEIJENStefaan De SmedtT MEYVISJo DemeesterJ KRIJGSVELDS ZAATJ DANKERT

A1 Journal Article in MACROMOLECULES

Fluorescence recovery after photobleaching: a versatile tool for mobility and interaction measurements in pharmaceutical research

Tom KL MeyvisStefaan De SmedtPatric Van OostveldtJo Demeester

A1 Journal Article in PHARMACEUTICAL RESEARCH

Rheological monitoring of long-term degrading polymer hydrogels.

T MEYVISStefaan De SmedtJo DemeesterWE HENNINK

A1 Journal Article in JOURNAL OF RHEOLOGY

1998

Hyaluronan: Preparation, structure, properties, and applications.

L LAPCIKStefaan De SmedtJo DemeesterP CHABRECEK

A1 Journal Article in CHEMICAL REVIEWS

1997

Deoxyribonuclease I

Ingrid De MeesterStefaan De Smedt

Bookchapter in Pharmaceutical enzymes

Diffusion of macromolecules in dextran methacrylate solutions and gels as studied by confocal scanning laser microscopy.

Stefaan De SmedtTom MeyvisJo DemeesterPatric Van OostveldtJCG BLONKWE HENNINK

A1 Journal Article in MACROMOLECULES

Moisture absorption and desorption of different rubber lyophilisation closures.

S CORVELEYNStefaan De SmedtJean Paul Remon

A1 Journal Article in INTERNATIONAL JOURNAL OF PHARMACEUTICS

1996

Controlled release of proteins from dextran hydrogels.

WE HENNINKH TALSMAJCH BORCHERTStefaan De SmedtJo Demeester

A1 Journal Article in JOURNAL OF CONTROLLED RELEASE

Stabilisation of suspensions using sucrose esters and low substituted n-octenylsuccinate starch-xanthan gum associations.

JD NTAWUKULILYAYOStefaan De SmedtJo DemeesterJean Paul Remon

A1 Journal Article in INTERNATIONAL JOURNAL OF PHARMACEUTICS

1995

A new approach in collecting diffusion data to support theological studies on gel like systems using hyaluronic acid and solutions as a model

Jo DemeesterStefaan De SmedtTom MeyvisP Van Oostveldt

Characterization of the network structure of dextran glycidyl methacrylate hydrogels by studying the rheological and swelling behavior

Stefaan De SmedtAlbert LauwersJo DemeesterMJ Van SteenbergenWE HenninkSPFM Roefs

A1 Journal Article in MACROMOLECULES

1994

Structural information on hydraulic acid solutions as studied by probe diffusion experiments.

Stefaan De SmedtAlbert LauwersJo DemeesterY ENGELBORGHSG DEMEYM DU

A1 Journal Article in MACROMOLECULES

1993

Viscoelastic and transient network properties of hyaluronic acid as a function of the concentration

Stefaan De SmedtP DekeyserV RibitschAlbert LauwersJo Demeester

A1 Journal Article in BIORHEOLOGY

1992

High Pressure, size-exclusion chromatography of Proteoglycans extracted from Bovine Articular Cartilage.

Philippe DekeyserStefaan De SmedtKoen VercruysseJo DemeesterAlbert Lauwers

Structural Rheology of Biopolymers

Stefaan De SmedtPhilippe DekeyserKoen VercruysseJo Demeester

Structural Rheology of Hyaluronic Acid

Stefaan De SmedtJo Demeester

Viscoölastic Properties of Hyaluronic Acid Solutions as a Function of Concentration

Stefaan De SmedtAlbert LauwersJo Demeester

Hyaluronidase Action on Hyaluronic Acid monitored by G.P.C. Abstract Intern. Conference of Enzymatic Synthesis and Modification of Carbohydrates, Coventry, U.K., 1992.

Koen VercruyssePhilippe DekeyserStefaan De SmedtAlbert LauwersJ DE MEESTER

Hyaluronidase action of hyaluronic acid monitored by G.P.C. Abstract of the Intern. Conference of Enzymatic Synthesis and Modification of Carbohydrates, Coventry, U.K., juli, 1992.

K VERCRUYSSEP DEKEYSERStefaan De SmedtA LAUWERSJ DE MEESTER