Levulinic acid is a promising bio-based platform chemical used in the production of various chemicals, including solvents, pharmaceuticals, polymers, and biofuels. However, the current production methods are the main bottleneck for their widespread industrial implementation as they do not allow easy scale up. Besides, the present production methods have a high energy demand and require extensive down stream processing due to the present side-products. To enable large-scale and sustainable production, this PhD aims to develop a fermentation-based approach as an alternative to the existing processes.