Research Explorer

Project

Combined dual transcriptomic, proteomic and functional investigations to reveal host-pathogen interactions behind the virulence of foodborne pathogen Bacillus cereus
Code
01N05417
Duration
01 January 2017 → 31 October 2021
Funding
Regional and community funding: Special Research Fund
Research disciplines
  • Natural sciences
    • Other chemical sciences
    • Microbiology
    • Systems biology
  • Medical and health sciences
    • Laboratory medicine
    • Microbiology
    • Laboratory medicine
    • Nutrition and dietetics
    • Laboratory medicine
    • Microbiology
  • Agricultural and food sciences
    • Agricultural animal production
    • Food sciences and (bio)technology
Keywords
virulence toxins bacillus cereus
 
Project description

A toxin-mediated infection of foodborne Bacillus cereus requires direct contact of viable B. cereus with its host for in-situ production of diarrheal enterotoxins. This direct contact leads to a cross-talk between a pathogen and its host. The interaction will be investigated by functional, cytotoxic and transcriptomic approaches, using tools such as Seahorse flux analysis, RNAseq, microarrays, RT-PCR, ELISA and mass-spectrometry.