Because of their unique properties, γδ T cells present themselves as attractive alternatives to conventional αβ T cells as immunotherapeutic agents against various cancer types. In humans different γδ subsets have been identified but the molecular mechanisms that drive their specific developmental pathways are still poorly understood. Using state-of-the-art single cell multi-omics approaches and high-resolution spatial transcriptomic and imaging technologies, we aim to unravel the transcriptional regulators and signalling pathways that direct human γδ T cell development in vivo. We will functionally validate the impact of these molecular processes using in vitro models of human T cell development and test their  ability to steer the development of human T cell precursors towards a particular γδ-lineage subset. The results from this project will allow the design of novel strategies for the generation of specific and naïve γδ subsets with specialised and prolonged effector functions for establishing tailored cancer immunotherapy.