Project

Biohydrogenation of docosahexaenoic acid in the rumen: identification of the intermediates and microbes involved

Duration
01 September 2016 → 31 December 2017
Funding
Regional and community funding: Special Research Fund
Research disciplines
  • Natural sciences
    • Other chemical sciences
    • Animal biology
    • General biology
    • Microbiology
    • Systems biology
  • Medical and health sciences
    • Laboratory medicine
    • Microbiology
    • Laboratory medicine
    • Nutrition and dietetics
    • Laboratory medicine
    • Microbiology
  • Agricultural and food sciences
    • Agricultural animal production
    • Food sciences and (bio)technology
    • Veterinary medicine
Keywords
rumen metabolism docosahexaenoic acid microorganisms
 
Project description

The project focuses on the poorly understood microbial 22:6n-3 biohydrogenation in the rumen. Extensive biohydrogenation occurs in vivo, but in vitro culture conditions had to be optimized to allow 22:6n-3 metabolism and identification of rumen biohydrogenating bacteria, both with pure and mixed cultures, the latter using the dilution-to-extinction approach. Finally, products formed during 22:6n-3 biohydrogenation are being identified using mass spectroscopy.