This study aims to investigate the cell type specific role of FOXG1, a neurodevelopmental transcription factor linked to various neurological disorders, using an integrated multi-methodological approach. First, we will establish an innovative, non-destructive method to identify different cell-types in live neural organoids based on fluorescence lifetime imaging microscopy (FLIM) of autofluorescent FAD molecules. Next, we will localize FOXG1 during neuronal development on a cellular and intra-cellular level and determine its cell-type specific interaction partners and DNA binding sites. Further, Human induced pluripotent stem cells will be CRISPR-Cas9 genetically engineered to harbour various FOXG1 aberrations will be used to generate neural organoids. Subsequently, these organoids will undergo non-destructive advanced imaging analyses (FLIM) and single-cell RNA sequencing to identify affected cell populations and aberrant transcriptomic event. Lastly, the study will investigate the potential of small activating RNAs to reverse the effects of FOXG1 loss of function in these neural organoids. In sum, the results of this study will deepen our understanding of the role of FOXG1 in neurodevelopment and disease, define a consistent pipeline for mixed-method organoid analysis of neurodevelopmental disorders, and may contribute to the development of novel therapeutic approaches for FOXG1 syndrome.