Research Explorer

Project

Derivation of embryonic stem cells from SPRET/Ei, an inbred strain from the wild species Mus spretus
Code
31516408
Duration
01 January 2008 → 31 December 2010
Funding
Research Foundation - Flanders (FWO)
Research disciplines
  • Natural sciences
    • Genetics
  • Medical and health sciences
    • Gynaecology and obstetrics
    • Molecular and cell biology
    • Morphological sciences
    • Gynaecology and obstetrics
    • Molecular and cell biology
    • Morphological sciences
    • Gynaecology and obstetrics
    • Molecular and cell biology
    • Morphological sciences
Keywords
ES-cells ivf SPRET/Ei
 
Project description
 

We want to derive ES-cells from the mouse strain SPRET/Ei. This way, we want to expand the toolbox of techniques and applications to genetically manipulate mammals. We will do this by isolating preantral follikels from young SPRET-Ei females and let them mature in vitro. The matured oocytes will then be fertilized in vitro with SPRET/Ei sperm and the rsulting zygotes will be kept in culture till the blastocyst stage. These blastocysts will then be used to derive SPRET/Ei ES-cells.