Development of innovative detection methods for ergot alkaloids in grain and grain products

01 December 2008 → 30 November 2011
Federal funding: various
Research disciplines
  • Natural sciences
    • Plant biology
  • Agricultural and food sciences
    • Agricultural plant production
    • Horticultural production
ergot alkaloids
Project description

The project aims to develop innovative detection methods for ergot alkaloids. On the one hand, there is a need for a validated fixing method in which all the representative ergot alkaloids to be analyzed; it is also necessary to provide fast, affordable screening. The research is divided into six work packages. In WP1 be ergot alkaloid reference standards selected for LC-MS / MS analysis and the most suitable template molecule is chosen for the development of aptamers, MIPs, respectively. In WP2 is a developed LC-MS / MS method for the simultaneous analysis of the selected ergot alkaloids. The method is optimized and validated for grain and feed languages. In WP3 is developed a group-selective MIP, so with selectivity for the entire group of the ergot alkaloids. A MIP solid phase extraction procedure (SPE-MIP) will be developed in which binding capability, cross-reactivity, and optimal washing and elution will be determined. This MIP-SPE procedure is as an alternative sample preparation step, coupled to the LC-MS / MS method. In WP4, a group of selective aptamer DNA can be isolated. Further, this group-selective aptamer will also be used for the development of an ergot-specific aptamer. In WP5 will MIPs and aptamers are characterized by means of SPR and QCM. On the basis of these data, the selection will be made of the most appropriate aptamer or MIP (if necessary with feedback to the development of better performing materials). In WP6 is developed an aptamer-based fast screening assay - type of lateral flow dipstick -, optimized, and validated for grain and feed languages.