Project

Lab equipment necessary for the generation, manipulation and genome editing of pluripotent stem cells

Code
01B11015
Duration
01 June 2015 → 31 May 2018
Funding
Regional and community funding: Special Research Fund
Research disciplines
  • Natural sciences
    • Scientific computing
    • Bioinformatics and computational biology
    • Genetics
    • Systems biology
  • Social sciences
    • Biological and physiological psychology
    • Cognitive science and intelligent systems
    • Developmental psychology and ageing
  • Medical and health sciences
    • Bioinformatics and computational biology
    • Molecular and cell biology
    • Neurosciences
    • Bioinformatics and computational biology
    • Neurosciences
    • Public health care
    • Public health services
    • Bioinformatics and computational biology
    • Molecular and cell biology
    • Neurosciences
  • Engineering and technology
    • Scientific computing
Keywords
Induced pluripotent stem cells (iPSC) Transfection Embryonic stem cells Genome editing
 
Project description

A specialized Laminar Air Flow with integrated microscope & a CO2 incubator are necessary for the generation, maintenance and manipulation of pluripotent stem cells. For genome editing and other manipulations of these stem cells, a nucleofector is the best instrument. Via nucleofection constructs are brought directly into the nucleus, which then can generate stable knockouts (or others).