Third-generation or long-read sequencing (LRS) was elected as Method of the Year 2022. This groundbreaking technology has revolutionized life science research, with an accuracy, throughput, extremely long reads and real-time data analysis that dramatically increased while costs remained low. LRS became an unprecedented technology to sequence complex genomic regions, structural variations, full genomes with higher accuracy and resolution, transcriptomes with alternative splicing events, fusion transcripts, novel isoforms, translatomes, epigenetic modifications, including DNA methylation. The Revio instrument (PacBio) is considered to offer the best LRS solutions for (epi)genomes, (epi)transcriptomes and translatomes. As LRS benefits researchers in almost all life science fields, we bring together the ThirdGenT consortium. This will link LRS with UGent experts in sequencing applications in life sciences, in particular oncology, rare diseases, microbiology, stem cell and gene technology, developmental and computational biology, paving the way for novel research avenues. The Revio equipment will be hosted by the UGent Sequencing Core, which has the required critical mass (infrastructure, operational capacity, expertise, personnel, technology, sample volume and turnover). The entire academic and business research community will take advantage of this innovative equipment, which will be the first in Flanders, hence catalysing local, national and international collaborations.