The hypothesis of my proposal is that regulation of bacterial physiology by epigenetic regulation of gene expression provided by DNA methylation contributes to the decreased susceptibility towards antimicrobial agents observed in bacterial biofilms. With my research I expect to obtain a fundamental understanding of how DNA methylation contributes to biofilm resistance in complex clinical environments, and I aim to provide proof-of-concept that DNA methylation pathways are a useful target for innovative antimicrobial therapy.