Rhizogenic Agrobacteria contain a root-inducing (Ri) plasmid, for which the naturally occurring ‘wild-type’ T-DNA encodes an array of genes that prompt transformed cells to rapidly form ‘hairy roots’ (HR). By equipping the Agrobacteria with a binary vector containing an additional T-DNA, HR can be produced that express genes of interest, including CRISPR/Cas9 components. Hence HR are often used in plant research, including for gene editing. Many dicot plants can easily be transformed using wild-type Agrobacterium rhizogenes and for some species, fertile plants can be regenerated. However, in many cases regeneration requires painstaking optimization of tissue culture conditions. Here, we aim to improve the efficiency and time needed to form transgenic and gene edited shoots from HRs (‘root to shoot’ R2S approach) using (i) the use of morphogenic regulators (MRs) as tools to improve regeneration, (ii) engineering of Agrobacteria, and (iii) CRISPR-based methods. Ultimately, we aim to develop a generic methodology for dicot transformation and gene editing, largely independent of species and genotype. Such methods are crucial for fulfilling the potential of gene editing in breeding and agriculture.