Project

Development of a module to study the gut biofilm formation and the host-microbiota interaction (Type 2)

Duration
01 June 2009 → 31 May 2011
Funding
Regional and community funding: IWT/VLAIO
Promotor
Research disciplines
  • Medical and health sciences
    • Gastro-enterology
    • Microbiome
Keywords
biofilm formation
 
Project description

The aim of the present project is the development of an in vitro cell culture-based system that biofilm formation and host - interaction microbiota in the intestinal lumen simulates biologically relevant way. In order to achieve this is to emulate the system has two important properties of the gastro-intestinal tract (GI): (i) the absorption of molecules from the different digestion steps, and (ii) bacterial adhesion to the gastheerepitheel by means of a muscuslaag. For this purpose, two modules are presented, on the one hand a mucusadhesie-module and on the other hand two host-microbiota signal modules with viable cell lines. The mucusadhesie module adhesion, kolonisaite, growth, and stabilization of microbial communities to a mucus layer must be applied to allow a semi-permeable membrane, so that the absorption of molecules by the gastheerepitheellaag can be simulated and studied. The host-microbiota module allows, by integration of enterocyte cell lines, to the study of the interaction between the (host) cells and the intestinal microbial community (s). This module will be made up of a microbial biofilm, a mucus layer, an optional artificial semi-permeable membrane, and a layer of living enterocytes. The whole must allow to form a realistic picture of the uptake of molecules from the gastro-intestinal tract on the one hand, and on the other hand the mutual signal transfer between the erythrocytes and the microbial community (ies), which thus probably goes hand in hand. In the absence of the semi-permeable membrane, there is direct contact between the microbiology, the mucus layer and the ery trocyten, which is closer to the reality, but because of stability reasons, it can only be used for short-life experiments. Separation of the mucus biofilm layer of erythrocytes through the semipermeable membrane will allow to keep the system more stable and therefore suitable for long-term experiments.