Water is a vital ingredient of our planet and essential in everyday life. However, only 1% of the water on the Earth is available as freshwater for mankind. Due to climate change and extreme weather, the 1% will further decrease. On the other hand, the anthropogenic pollution of this valuable resource increases, causing a possible threat to the environment and human health. The pollutants, such as organic emerging contaminants and microplastics, end up in effluent due to poor removal efficiency of currently applied wastewater treatment techniques. To preserve environmental water quality improved techniques and methods are necessary. Specifically, enzymes need more scientific research, because of their capacity to catalyse chemically challenging reactions. In particular, radical-based enzymatic degradation of pollutants is described as a promising method, due to the high reactivity of radical molecules causing chain reactions and eventual degradation of the substrate. However, knowledge about the performance of a selection of (novel) radical enzymes fed with a mixture of organic pollutants under various physicochemical conditions is still lacking. This research aims to fill the knowledge gaps while focusing on realistic operational conditions.