The HCRC will receive samples from Emory University in this project (extracted DNA and RNA and plasma). HCRC will perform total HIV DNA, intact proviral DNA assay analysis and CA RNA analysis using a higher order multiplex platform (5 color). HCRC will perform single genome sequencing of plasma virus (SGS). In addition, HCRC will perform -unique modified identifier- nanopore based sequencing to obtain near full length sequences in semi high throughput manner. HCRC will perform MIP-seq which evaluates HIV integration sites and linked proviral sequences; or regular ISLA when needed. Finally, HCRC will validate new findings by CRISPR-CAS mediated knockout of new targets to be cross validated in this project.