qPCR for gene expression analyses

15 December 2022 → 14 December 2024
Regional and community funding: Special Research Fund
Research disciplines
  • Natural sciences
    • Phytopathology
    • Plant genetics
    • Plant immunology
  • Agricultural and food sciences
    • Agricultural plant protection
    • Crop science
RNA molecular biology gene expression analysis
Project description

QPCR is one of the basic techniques to investigate gene expression. With this application, we aim at replacing the current machine which is already in shared use for 8 years by the research groups of  professors Tina Kyndt, and Godelieve Gheysen (FBE). The current machine is used on a daily basis, by all researchers in both teams, but is currently suffering from technical issues. The qPCR machine that we are applying for here would consist of multiple detectors, making the equipment more specific and more versatile than the current version, which can only detect SYBR Green. This will allow using the machine for multiplex analyses.

This machine is an unvaluable piece of equipment that will be used in all running and future research projects. Almost every recent publication from both research groups includes at least 1 qPCR-based experiment.