Wide-field microscopy is used for thin samples, provides low light exposure and can acquire frames with a speed in the order of 20fps. With this microscope, the sample is illuminated by a collimated beam of light.

The instrument is equipped for both brightfield (phase contrast, DIC) and fluorescence imaging.

Specifications:

• Inverted
• Objectives:
  • CFI Plan Fluor DL 4XF, NA 0.13, WD 16.5 (MRH20045)
  • CFI Plan Fluor DLL 10X, NA 0.3, WD 16 (MRH10105)
  • CFI Plan Apochromat Lambda D 20X, NA 0.75, WD 1 (MRD70270)
  • 40X
• Filter sets (UV to red):
  • DIA/DIC ANALYSER
  • DAPI (Ex. 387/11, Em. 447/60)
  • GFP-B (Ex. 472/30, Em. 520/35)
  • TRITC (Ex. 543/22, Em. 593/40)
  • Cy5 (Ex. 628/40, Em. 692/40)
  • GFP-L (472/30, Em. 488LP)
• Two cameras:
  • Light sensitive, cooled EM-CCD camera (1000*1000 pixels)
  • Nikon colour CMOS camera
• Fully automatic filter wheels, objectives, XY-Stage, light source, …
• Incubator with temperature regulation at the microscope level and CO2 at the cell culture level
• Perfect Focus System
• Extended software for recording and visualization of multi-dimensional (X, Y, Z, Lambda, t) images (NIS elements)