Confocal laser scanning microscopy (CLSM) is a widely used technique in the life sciences for making high-quality images of biological samples. The samples are illuminated by a focused laser beam which is scanned over the sample in order to visualize fluorescent markers. Emission from each position is detected by a point detector and a pinhole removes out of focus emission light to improve contrast and resolution. CLSM results in optical sectioning and a 3D reconstruction of the sample can be made.

The Nikon A1R has the additional possibilities to scan very fast with its resonant scanner (30 FPS), to observe scattering particles and to perform spectral imaging and lineair unmixing.

Specifications:

• Objectives:
  • 10X plan apo AIR dic N1(NA 0,45; WD 4; brightness 4,10) MRD70170
  • 20X plan fluor AIR dic N2 (NA 0,5; WD 2,1; brightness 1,56) MRH00205
  • 40X S plan fluor ELWD AIR (NA 0,6; WD 3,6-2,8; brightness 0,81) MRH08430
  • 40X plan fluor OIL dic H/N2 (NA 1,3; WD 0.2; brightness 17,85) MRH01401
  • 60X plan apo VC OIL dic N2 (NA 1,4; WD 0.130; brightness 10,67)
  • 60X plan apo VC H2O dic N2 (NA 1,2; WD 0,31-0,28; brightness 5,76) MRD07602
• Lasers:
  • 4 lasers with in total 7 laser lines: 405 nm, 457 nm, 476 nm, 488 nm, 514 nm, 561 nm, 639 nm
  • 2 separated lasers for Controlled Light Exposure Microscopy (488 & 543 nm)
• Scanners:
  • Galvoscanner
  • Resonantscanner.
• Simultaneous use possible for photo-stimulation experiments (FRAP, FRET, ...)
• Detectors:
  • Simultaneous 2-channel GAaSP en 2-channel PMT detection
    • Channel 1: 450/50 or 482/35
    • Channel 2: 525/50 or 540/30 or 515/30
    • Channel 3: 595/50 or 585/65
    • Channel 4: 700/75
  • 32-channel PMT spectral detector with resolution of 2.5, 6 or 10 nm per channel
• Piezo stage for fast and precise scanning in the z direction
• Perfect Focus System
• Incubator with temperature regulation at the microscope level and CO2 at the cell culture level