The Synapt G2-Si is a versatile Q-TOF device comprising Ion Mobility separation (IMS) and Electron Transfer Dissociation (ETD) capabilities. It allows for a plethora of different acquisition strategies, the most prominent of which are HDMSE or ion mobility-assisted data-independent acquisition and HDDDA or ion mobility-assisted data-dependent acquisition. Herein, both identification and quantitation is done on DIA data. The Synapt G2-Si is coupled to a Nano Acquity LC-system, capable of both capillary- and nano-flow LC.