Research Explorer

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Researcher

Nico Callewaert

Publications & Research Data

Awards & Distinctions

232 Results

2025

Glycomics as prognostic biomarkers of hepatocellular carcinoma : a systematic review

Nicky SomersEmma ButayeLorenz GrossarNele PauwelsAnja GeertsSarah RaevensSander LefereLindsey DevisscherLeander MeurisNico Callewaert et al.

A1 Journal Article in ONCOLOGY LETTERS

Impact of IgG Fc glycosylation on disease dynamics in patients with primary Sjögren disease

Helena AchtenLeander MeurisLiselotte DerooMatthias JarlborgTine DecruyJoke DeprezEmilie DumasKristel De BoeckEva GenbruggeWouter Bauters et al.

A1 Journal Article in ARTHRITIS & RHEUMATOLOGY

Single-domain antibodies directed against hemagglutinin and neuraminidase protect against influenza B viruses

Arne MatthysJan FélixJoão Portela CataniKenny RooseWim NerinckxBenthe Van BuytenDaria FijalkowskaNico CallewaertSavvas SavvidesXavier Saelens

A1 Journal Article in NATURE COMMUNICATIONS

Ultrapotent SARS coronavirus-neutralizing single-domain antibodies that clamp the spike at its base

Sieglinde De CaeInge Van MolleLoes van SchieSophie R. ShoemakerJulie DeckersNincy DebeufSahine LameireWim NerinckxKenny RooseDaria Fijalkowska et al.

A1 Journal Article in NATURE COMMUNICATIONS

2024

A fast, affordable and minimally-invasive diagnostic test for Cancer of Unknown Primary (CUP) using DNA methylation profiling

Jilke De WildeRuben Van PaemelAndries De KokerSofie RoelandtSofie Van de VeldeNico CallewaertJo Van DorpeDavid CreytensBram De WildeKatleen De Preter

A1 Journal Article in LABORATORY INVESTIGATION

Advancing diagnosis and early risk assessment of preeclampsia through noninvasive cell-free DNA methylation profiling

Machteld BaetensBram Van GaeverStephanie DeblaereAndries De KokerLeander MeurisNico CallewaertSandra JanssensKristien RoelensEllen RoetsJo Van Dorpe et al.

A1 Journal Article in CLINICAL EPIGENETICS

Exploration of yeast-produced edible antibodies for the management of gastrointestinal diseases

Charlotte RoelsNico CallewaertKenny RooseKatrien Claes

Integrating artificial intelligence-based epitope prediction in a SARS-CoV-2 antibody discovery pipeline : caution is warranted

Delphine Diana AcarWojciech WitkowskiMagdalena WejdaRuifang WeiTim DesmetBert SchepensSieglinde De CaeKoen SedeynHannah EeckhautDaria Fijalkowska et al.

A1 Journal Article in EBIOMEDICINE

Massively parallel analysis of protein libraries using yeast surface display

Hannah EeckhautNico CallewaertJasper Zuallaert

MX2 forms nucleoporin-comprising cytoplasmic biomolecular condensates that lure viral capsids

Georgios MoschonasLouis DelhayeRobin CooremanFranziska HuesersAnayat BhatZoi StylianidouElien De BousserLaure De PryckHanna GrzesikDelphine De Sutter et al.

A1 Journal Article in CELL HOST & MICROBE

OPENPichia : licence-free Komagataella phaffii chassis strains and toolkit for protein expression

Katrien ClaesDries Van HerpeRobin VanlucheneCharlotte RoelsBerre Van MoerElise WyseureKristof VandewalleHannah EeckhautSemiramis YilmazSandrine Vanmarcke et al.

A1 Journal Article in NATURE MICROBIOLOGY

Pre-treatment serum N-glycans predict poor immunotherapy response and survival in hepatocellular carcinoma

Nicky SomersTheresa HoltmannFabian ArtusaEmma ButayeLorenz GrossarSarah RaevensAnja GeertsSander LefereLindsey DevisscherLeander Meuris et al.

Specific glycomic profiles can identify patients at risk of poor transplant-free survival in primary biliary cholangitis

Nicky SomersNikolaos GatselisMaria C. van HooffVasiliki LygouraEmma ButayeLorenz GrossarSarah RaevensAnja GeertsSander LefereLindsey Devisscher et al.

Systematic review : glycomics as diagnostic markers for hepatocellular carcinoma

Emma ButayeNicky SomersLorenz GrossarNele PauwelsSander LefereLindsey DevisscherSarah RaevensAnja GeertsLeander MeurisNico Callewaert et al.

A1 Journal Article in ALIMENTARY PHARMACOLOGY & THERAPEUTICS

The association between urine N-glycome and prognosis after initial therapy for primary prostate cancer

Tijl VermassenNicolaas LumenCharles Van PraetNico CallewaertJoris DelangheSylvie Rottey

A1 Journal Article in BIOMEDICINES

The association between urine N-glycome and prognosis after initial therapy for primary prostate cancer

Tijl VermassenNicolaas LumenCharles Van PraetNico CallewaertJoris DelangheSylvie Rottey

A1 Journal Article in BIOMEDICINES

2023

A metabolic gene survey pinpoints fucosylation as a key pathway underlying the suppressive function of regulatory T cells in cancer

Sotiria PiniotiHimal SharmaNina C. FlerinQian YuAmalia TzoumpaSarah Trusso CafarelloElien De BousserNico CallewaertGuillaume OldenhoveSusan Schlenner et al.

A1 Journal Article in CANCER IMMUNOLOGY RESEARCH

Glycomics-based serum markers as reliable tool for assessment of viral response after treatment with direct-acting antiviral drugs in hepatitis C virus infection

Nicky SomersElisabeth VandekerckhoveAnja GeertsHelena DegrooteSander LefereLindsey DevisscherLeander MeurisNico CallewaertHans Van VlierbergheXavier Verhelst

A1 Journal Article in ACTA CLINICA BELGICA

GlycoVHH : optimal sites for introducing N-glycans on the camelid VHH antibody scaffold and use for macrophage delivery

Loes van SchieWander Van BreedamCharlotte RoelsBert SchepensMartin FrankAhmadreza MehdipourBram LaukensWim NerinckxFrancis SantensSimon Devos et al.

A1 Journal Article in MABS

Harnessing deep mutational scanning for faster mapping of protein-protein interaction interfaces

Stefanie MaesFrank PeelmanNico Callewaert

Improving therapeutic proteins by N-glycosylation engineering and conjugation

Berre Van MoerNico CallewaertKenny Roose

Modulating antibody effector functions by Fc glycoengineering

Mikel García-AlijaBerre Van MoerDiego E. SastreTala AzzamJonathan J. DuBeatriz TrastoyNico CallewaertEric J. SundbergMarcelo E. Guerin

A1 Journal Article in BIOTECHNOLOGY ADVANCES

N-glycosylation engineering in chimeric antigen receptor T cells enhances anti-tumor activity

Elien De BousserNele FestjensLeander MeurisEvelyn PletsAnnelies Van HeckeElise WyseureStijn De MunterBart VandekerckhoveNico Callewaert

Novel glycoproteomics methodology for discovering tumor-specific, cell surface, glycopeptide epitopes

Wannes WeytsNico CallewaertKris GevaertSimon Devos

PhosphoLingo : protein language models for phosphorylation site prediction

Jasper ZuallaertPathmanaban RamasamyRobbin BouwmeesterNico CallewaertSven Degroeve

2022

Developing methods for the production of antiviral VHH-Fc antibodies in Pichia pastoris for fast pandemic response

Chiara LonigroNico CallewaertXavier Saelens

GENType : all-in-one preimplantation genetic testing by pedigree haplotyping and copy number profiling suitable for third-party reproduction

Lisa De WitteLennart RamanMachteld BaetensAndries De KokerNico CallewaertSofie SymoensKelly TillemanFrauke Vanden MeerschautAnnelies DheedeneBjörn Menten

A1 Journal Article in HUMAN REPRODUCTION

Glycomics-based serum marker as reliable tool for assessment of viral response after treatment with direct-acting antiviral drugs in hepatitis C virus infection

Nicky SomersElisabeth VandekerckhoveAnja GeertsHelena DegrooteSander LefereLindsey DevisscherLeander MeurisNico CallewaertHans Van VlierbergheXavier Verhelst

GlyConnect-Ugi : site-selective, multi-component glycoprotein conjugations through GlycoDelete expressed glycans

Karel ThooftWander Van BreedamFrancis SantensElise WyseureSandrine VanmarckeSimon DevosNico CallewaertAnnemieke Madder

A1 Journal Article in ORGANIC & BIOMOLECULAR CHEMISTRY

GlycoVHH : introducing N-glycans on the camelid VHH antibody scaffold : optimal sites and use for macrophage delivery

Loes van SchieWander Van BreedamCharlotte RoelsBert SchepensMartin FrankAhmadreza MehdipourBram LaukensWim NerinckxFrancis SantensSimon Devos et al.

Pretransplant alterations in serum protein glycosylation are associated to risk of hcc recurrence after liver transplantation and provide a potential prognostic biomarker : a proof-of-concept study

Xavier VerhelstHeleen EngelsAllison GeertsAude VanlanderLuis Abreu De CarvalhoHelena DegrooteLeander MeurisFrederik BerrevoetNico CallewaertHans Van Vlierberghe

Pretransplant changes in serum protein glycosylation relate to risk of HCC recurrence after liver transplantation and provide a potential prognostic biomarker : a proof-of-concept study

Xavier VerhelstHeleen EngelsAnja GeertsAude VanlanderLuis Abreu De CarvalhoHelena DegrooteLeander MeurisFrederik BerrevoetNico CallewaertHans Van Vlierberghe

The 3-O sulfation of heparan sulfate proteoglycans contributes to the cellular internalization of tau aggregates

Andreia Sofia da Costa de Monica FerreiraInes RoyauxJian LiuZhangjie WangGuowei SuDiederik MoecharsNico CallewaertLouis De Muynck

A1 Journal Article in BMC MOLECULAR AND CELL BIOLOGY

Validation of a glycomics-based test associated with risk of HCC development in cirrhosis

Xavier VerhelstLeander MeurisRoos ColmanAnnelies Van HeckeAnja GeertsNico CallewaertHans Van Vlierberghe

Yeast-produced fructosamine-3-kinase retains mobility after ex vivo intravitreal injection in human and bovine eyes as determined by Fluorescence Correlation Spectroscopy

An-Katrien MinnaertLoes van SchieHendrik GrootaertJonas HimpeSimon DevosWannes WeytsHerlinde De KeersmaeckerKevin BraeckmansElisabeth Van AkenJoris Delanghe et al.

A1 Journal Article in INTERNATIONAL JOURNAL OF PHARMACEUTICS

2021

A potential role for fructosamine-3-kinase in cataract treatment

Sander De BruyneLoes van SchieJonas HimpeFilip De SomerInge EveraertWim DeraveCaroline Van den BroeckeManon HuizingNezahat BostanMarijn Speeckaert et al.

A1 Journal Article in INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

An affinity-enhanced, broadly neutralizing heavy chain-only antibody protects against SARS-CoV-2 infection in animal models

Bert SchepensLoes van SchieWim NerinckxKenny RooseWander Van BreedamDaria FijalkowskaSimon DevosWannes WeytsSieglinde De CaeSandrine Vanmarcke et al.

A1 Journal Article in SCIENCE TRANSLATIONAL MEDICINE

Engineering of yeast glycoprotein expression

Charlot De WachterLinde Van LanduytNico Callewaert

A1 Journal Article in ADVANCES IN BIOCHEMICAL ENGINEERING-BIOTECHNOLOGY

Epidermal galactose spurs chytrid virulence and predicts amphibian colonization

Yu WangElin VerbruggheLeander MeurisKoen ChiersMoira KellyDiederik StrubbeNico CallewaertFrank PasmansAn Martel

A1 Journal Article in NATURE COMMUNICATIONS

Exploration of synergistic action of cell wall-degrading enzymes against mycobacterium tuberculosis

Loes van SchieKatlyn BorgersGitte MichielsenEvelyn PletsMarnik VuylstekePetra TielsNele FestjensNico Callewaert

A1 Journal Article in ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

FRI0304 serum IGG-undergalactosylation profiles reflect cumulative exposure to systemic inflammation in spondyloarthritis patients

Ann-Sophie De CraemerZ. LukasikL. MeurisLiselotte DerooThomas RensonManouk de HoogePhilippe CarronAnnelies Van HeckeNico CallewaertFilip Van den Bosch et al.

GlyConnect : exploring glycan-based conjugation chemistries for GLycoDelete proteins

Karel ThooftAnnemieke MadderNico CallewaertWander Van Breedam

Knockout of RSN1, TVP18 or CSC1-2 causes perturbation of Golgi cisternae in Pichia pastoris

Rochelle AwCharlot De WachterBram LaukensRiet De RyckeMichiel De BruyneDavid BellNico CallewaertKaren M. Polizzi

A1 Journal Article in TRAFFIC

Massively parallel interrogation of protein fragment secretability using SECRiFY reveals features influencing secretory system transit

Morgane BoonePathmanaban RamasamyJasper ZuallaertRobbin BouwmeesterBerre Van MoerDavy MaddeleinDemet TuranNiels HulstaertHannah EeckhautElien Vandermarliere et al.

A1 Journal Article in NATURE COMMUNICATIONS

Method development for the structural and functional analysis of (CAR) T cell glycome alterations

Elien De BousserNico CallewaertNele Festjens

Minimally invasive classification of pediatric solid tumors using reduced representation bisulfite sequencing of cell-free DNA : a proof-of-principle study

Ruben Van PaemelAndries De KokerCharlotte VandeputteLieke van ZogchelTim LammensGenevieve LaureysJo VandesompeleGudrun SchleiermacherMathieu ChicardNadine Van Roy et al.

A1 Journal Article in EPIGENETICS

Ring finger protein 213 assembles into a sensor for ISGylated proteins with antimicrobial activity

Fabien Henri TheryLia MartinaCaroline AsselmanHeidi RepoY ZhangKoen SedeynGeorgios MoschonasC BredowQW TeoJ Zhang et al.

A1 Journal Article in NATURE COMMUNICATIONS

Serum glycomics on postoperative day 7 are associated with graft loss within 3 months after liver transplantation regardless of early allograft dysfunction

Xavier VerhelstAnja GeertsRoos ColmanAude VanlanderHelena DegrooteLuis Abreu De CarvalhoLeander MeurisFrederik BerrevoetXavier RogiersNico Callewaert et al.

A1 Journal Article in TRANSPLANTATION

Single-domain antibodies for biopharmaceutical targeting to the lysosome

Linde Van LanduytNico Callewaert

T cell engaging immunotherapies, highlighting chimeric antigen receptor (CAR) T cell therapy

Elien De BousserNico CallewaertNele Festjens

A1 Journal Article in CANCERS

Tissue N-linked glycosylation as potential prognostic biomarker for biochemical recurrence-free survival

Tijl VermassenArne Van den BroeckNicolaas LumenNico CallewaertSylvie RotteyJoris Delanghe

A1 Journal Article in BIOMARKERS

2020

Automated learning of biologically relevant features from primary sequence using convolutional neural networks

Jasper ZuallaertWesley De NeveNico CallewaertYvan Saeys

Development of a counterselectable transposon to create markerless knockouts from an 18,432-clone ordered Mycobacterium bovis bacillus Calmette-Guérin mutant resource

Katlyn BorgersKristof VandewalleAnnelies Van HeckeGitte MichielsenEvelyn PletsLoes van SchieSandrine VanmarckeLaurent SchindfesselNele FestjensNico Callewaert

A1 Journal Article in MSYSTEMS

Development of molecular tools for Komagataella phaffii and exploring its use for orally administered biopharmaceuticals

Robin VanlucheneNico Callewaert

Functional exploration of the GH29 fucosidase family

Hendrik GrootaertLinde Van LanduytPaco HulpiauNico Callewaert

A1 Journal Article in GLYCOBIOLOGY

Human T cell glycosylation and implications on immune therapy for cancer

Elien De BousserLeander MeurisNico CallewaertNele Festjens

A1 Journal Article in HUMAN VACCINES & IMMUNOTHERAPEUTICS

Minimally invasive classification of pediatric solid tumors using reduced representation bisulfite sequencing of cell-free DNA

Ruben Van PaemelAndries De KokerCharlotte VandeputteLieke Van ZogchelTim LammensGenevieve LaureysJo VandesompeleGudrun SchleiermacherMatthieu ChicardNadine Van Roy et al.

Off-target glycans encountered along the synthetic biology route toward humanized N-glycans in Pichia pastoris

Bram LaukensPieter JacobsKatelijne GheysenJosé MartinsCharlot De WachterPaul AmelootWilly MorelleJurgen HaustraeteJean-Christophe RenauldBart Samyn et al.

A1 Journal Article in BIOTECHNOLOGY AND BIOENGINEERING

Protein glycosylation as a diagnostic and prognostic marker of chronic inflammatory gastrointestinal and liver diseases

Xavier VerhelstAna M DiasJean-Frederic ColombelSeverine VermeireHans Van VlierbergheNico CallewaertSalome S Pinho

A1 Journal Article in GASTROENTEROLOGY

Serum IGG-Undergalactosylation profiles reflect cumulative exposure to systemic inflammation in Spondyloarthritis patients

A. S. De CraemerZ. LukasikLeander MeurisL. DerooT. RensonManouk de HoogeP. CarronAnnelies Van HeckeNico CallewaertFilip Van den Bosch et al.

Single-chain soluble receptor fusion proteins as versatile cytokine inhibitors

Aurora Holgado MuñozHarald BraunKenneth VerstraeteDomien VannesteNico CallewaertSavvas SavvidesInna AfoninaRudi Beyaert

A1 Journal Article in FRONTIERS IN IMMUNOLOGY

SLC37A4-CDG : mislocalization of the glucose-6-phosphate transporter to the Golgi causes a new congenital disorder of glycosylation

Thorsten MarquardtVladimir BzduchMax HogrebeStephan RustJanine ReunertMarianne GrünebergJulien ParkNico CallewaertRobin LachmannYoshinao Wada et al.

A1 Journal Article in MOLECULAR GENETICS AND METABOLISM REPORTS

STAT2 signaling restricts viral dissemination but drives severe pneumonia in SARS-CoV-2 infected hamsters

Robbert BoudewijnsHendrik Jan ThibautSuzanne J. F. KapteinRong LiValentijn VergoteLaura SeldeslachtsJohan Van WeyenberghCarolien De KeyzerLindsey BervoetsSapna Sharma et al.

A1 Journal Article in NATURE COMMUNICATIONS

Structural basis for potent neutralization of betacoronaviruses by single-domain camelid antibodies

Daniel WrappDorien De VliegerKizzmekia S. CorbettGretel M. TorresNianshuang WangWander Van BreedamKenny RooseLoes van SchieMarkus HoffmannStefan Poehlmann et al.

A1 Journal Article in CELL

Structural basis for potent neutralization of betacoronaviruses by single-domain camelid antibodies (vol 181, pg 1004, 2020)

Daniel WrappDorien De VliegerKizzmekia S. CorbettGretel M. TorresNianshuang WangWander Van BreedamKenny RooseLoes van SchieMarkus HoffmannStefan Pohlmann et al.

Structure and assembly mechanism of the signaling complex mediated by human CSF-1 (vol 23, pg 1621, 2015)

Jan FélixSteven De MunckKenneth VerstraeteLeander MeurisNico CallewaertJonathan ElegheertSavvas Savvides

Using GlycoDelete to produce proteins lacking plant-specific N-glycan modification in seeds (vol 33, pg 1135, 2019)

Robin PironFrancis SantensANNELIES DE PAEPEAnna DepickerNico Callewaert

2019

A fast, affordable and minimally-invasive diagnostic test for Cancer of Unknown Primary (CUP) using DNA methylation profiling

Jilke De WildeRuben Van PaemelCharlotte VandeputteAndries De KokerJoni Van der MeulenMalaïka Van der LindenNico CallewaertJo Van DorpeDavid CreytensBram De Wilde et al.

A guide to Mycobacterium mutagenesis

Katlyn BorgersKristof VandewalleNele FestjensNico Callewaert

A1 Journal Article in FEBS JOURNAL

A versatile method for circulating cell-free DNA methylome profiling by reduced representation bisulfite sequencing

Andries De KokerRuben Van PaemelBram De WildeKatleen De PreterNico Callewaert

Characterization of human recombinant N-acetylgalactosamine-6-sulfate sulfatase produced in Pichia pastoris as potential enzyme for Mucopolysaccharidosis IVA treatment

Alexander Rodríguez-LópezLuisa N Pimentel-VeraAngela J Espejo-MojicaAnnelies Van HeckePetra TielsShunji TomatsuNico CallewaertCarlos J Alméciga-Díaz

A1 Journal Article in JOURNAL OF PHARMACEUTICAL SCIENCES

Customized protein glycosylation to improve biopharmaceutical function and targeting

Linde Van LanduytChiara LonigroLeander MeurisNico Callewaert

A1 Journal Article in CURRENT OPINION IN BIOTECHNOLOGY

Development and implementation of novel genetic tools for slow-growing mycobacteria

Katlyn BorgersNico CallewaertNele Festjens

Exploring protein therapeutics to fight tuberculosis

Loes van SchieNico Callewaert

Faster, cheaper and more accurate diagnosis of cancer of unknown primary (CUP) by using methylation profiling.

Jilke De WildeRuben Van PaemelCharlotte VandeputteAndries De KokerJoni Van der MeulenMalaïka Van der LindenNico CallewaertJo Van DorpeDavid CreytensBram De Wilde et al.

Minimally invasive classification of pediatric solid tumors using reduced representation bisulfite sequencing of cell-free DNA : a proof-of-principle study

Ruben Van PaemelAndries De KokerCharlotte VandeputteLieke van ZogchelTim LammensGenevieve LaureysJo VandesompeleGudrun SchleiermacherMathieu ChicardNadine Van Roy et al.

N-Linked glycosylation and near-infrared spectroscopy in the diagnosis of prostate cancer

Tijl VermassenSander De BruyneJonas HimpeNicolaas LumenNico CallewaertSylvie RotteyJoris Delanghe

A1 Journal Article in INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Novel methods designed for diagnostic methylome profiling of blood circulating cell-free DNA : expanding the liquid biopsy molecular toolbox

Andries De KokerNico Callewaert

Prostate protein n-glycosylation profiling by means of DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis

Tijl VermassenNico CallewaertSylvie RotteyJoris Delanghe

Bookchapter in Clinical applications of capillary electrophoresis : methods and protocols

Reference genome and comparative genome analysis for the WHO reference strain for Mycobacterium bovis BCG Danish, the present tuberculosis vaccine

Katlyn BorgersJheng-Yang OuPo-Xing ZhengPetra TielsAnnelies Van HeckeEvelyn PletsGitte MichielsenNele FestjensNico CallewaertYao-Cheng Lin

A1 Journal Article in BMC GENOMICS

SapM mutation to improve the BCG vaccine : genomic, transcriptomic and preclinical safety characterization

Nele FestjensKristof VandewalleErica HouthuysEvelyn PletsDieter VanderschaegheKatlyn BorgersAnnelies Van HeckePetra TielsNico Callewaert

A1 Journal Article in VACCINE

Serum glycomics on postoperative day 7 are associated with graft loss 3 months after liver transplantation

Xavier VerhelstAnja GeertsRoos ColmanAude VanlanderLUIS ABREU DE CARVALHOFrederik BerrevoetRoberto TroisiXavier RogiersNico CallewaertHans Van Vlierberghe

The generation and use of recombinant extracellular vesicles as biological reference material

Edward GeeurickxJoeri TulkensBert DhondtJan Van DeunLien LippensGlenn VergauwenElisa HeyrmanDelphine De SutterKris GevaertFrancis Impens et al.

A1 Journal Article in NATURE COMMUNICATIONS

The potential of glycomics as prognostic biomarkers in liver disease and liver transplantation

Xavier VerhelstAnja GeertsNico CallewaertHans Van Vlierberghe

A1 Journal Article in ACTA GASTRO-ENTEROLOGICA BELGICA

Yeast-secreted, dried and food-admixed monomeric IgA prevents gastrointestinal infection in a piglet model

Vikram VirdiJorge Palací BatallerBram LaukensStefan RyckaertEric CoxErik VanderbekeAnna DepickerNico Callewaert

A1 Journal Article in NATURE BIOTECHNOLOGY

2018

Capturing the ‘ome’ : the expanding molecular toolbox for RNA and DNA library construction

Morgane BooneAndries De KokerNico Callewaert

A1 Journal Article in NUCLEIC ACIDS RESEARCH

Endoglycosidase S enables a highly simplified clinical chemistry procedure for direct assessment of serum IgG undergalactosylation in chronic inflammatory disease

Dieter VanderschaegheLeander MeurisTom RaesHendrik GrootaertAnnelies Van HeckeXavier VerhelstFrederique Van de VeldeBruno LapauwHans Van VlierbergheNico Callewaert

A1 Journal Article in MOLECULAR & CELLULAR PROTEOMICS

Exploring the influence of N-glycosylation on allergen-specific immunotherapy

Charlot De WachterNico CallewaertBart Lambrecht

Glycome patterns of perfusate in livers before transplantation associate with primary non-function

Xavier VerhelstAnja GeertsIna JochmansDieter VanderschaegheAgnes ParadissisAude VanlanderFrederik BerrevoetGéraldine DahlqvuistFrederik NevensJacques Pirenne et al.

A1 Journal Article in GASTROENTEROLOGY

Mechanical strain determines the site-specific direction of inflammation and tissue damage in arthritis

Isabelle CambréDjoere GaublommeArne BurssensPeggy JacquesDirk ElewautR LoriesG SchettG KolliasAmélie De MuynckLeander Meuris et al.

Mechanical strain determines the site-specific direction of inflammation and tissue damage in arthritis

Isabelle CambréDorien GaublommeArne BurssensPeggy JacquesLeander MeurisStijn LambrechtShea CarterAmélie De MuynckNico CallewaertGeorge Kollias et al.

Mechanical strain determines the site-specific localization of inflammation and tissue damage in arthritis

Isabelle CambréDjoere GaublommeArne BurssensPeggy JacquesNadia SchryversAmélie De MuynckLeander MeurisStijn LambrechtShea CarterPieter De Bleser et al.

A1 Journal Article in NATURE COMMUNICATIONS

Metabolic engineering of glycosylation and secretion pathways in Pichia pastoris and its application in production of the human recombinant enzyme N-acetylgalactosamine-6-sulfate sulfatase

Edwin Alexander Rodríguez LópezNico CallewaertCarlos Javier Alméciga Diaz

The potential of methylome sequencing in the diagnostic work : up of pediatric solid tumors

Ruben Van PaemelCharlotte VandeputteBram De WildeKatleen De PreterNadine Van RoyJo VandesompeleTim LammensWim Van CriekingeP. UltzNico Callewaert et al.

2017

A glycomic based serum marker analysed on postoperative day 7 after liver transplantation is an independent predictor of graft and patient survival during the first year after liver transplantation

Xavier VerhelstAnja GeertsXavier RogiersAude VanlanderFrederik BerrevoetNico CallewaertRoberto TroisiHans Van Vlierberghe

A glycomic serum marker analysed at one week after liver transplantation is an independent predictor of graft loss during the first year after liver transplantation

Xavier VerhelstAnja GeertsXavier RogiersAude VanlanderFrederik BerrevoetNico CallewaertRoberto TroisiHans Van Vlierberghe

A glycomic serum marker analysed at one week after liver transplantation is an independent predictor of graft loss during the first year after liver transplantation

Xavier VerhelstAnja GeertsXavier RogiersAude VanlanderFrederik BerrevoetNico CallewaertHans Van Vlierberghe

A glycomics-based test predicts the development of hepatocellular carcinoma in cirrhosis

Xavier VerhelstDieter VanderschaegheLaurent CasteraTom RaesAnja GeertsClaire FrancozRoos ColmanFrançois DurandNico CallewaertHans Van Vlierberghe

A1 Journal Article in CLINICAL CANCER RESEARCH

A pretransplant glycomic signature in perfusate is predictive of primary non function after liver transplantation

Xavier VerhelstAnja GeertsXavier RogiersAude VanlanderFrederik BerrevoetNico CallewaertRoberto TroisiHans Van Vlierberghe

A simple colorimetric assay for measuring fructosamine 3 kinase activity

Justin C CikomolaAntoine S KishabongoKarl VandepoeleMARIEKE DE MULDERPhilippe B KatchungaBram LaukensLoes van SchieHendrik GrootaertNico CallewaertMarijn Speeckaert et al.

A1 Journal Article in CLINICAL CHEMISTRY AND LABORATORY MEDICINE

An endoplasmic reticulum-engineered yeast platform for overproduction of triterpenoids

Philipp ArendtKarel MiettinenJacob PollierRiet De RyckeNico CallewaertAlain Goossens

A1 Journal Article in METABOLIC ENGINEERING

Exploring glyco-active enzymes for biomedical glycan manipulation

Hendrik GrootaertNico Callewaert

Hierarchical and redundant roles of activating FcγRs in protection against influenza disease by M2e-specific IgG1 and IgG2a antibodies

Silvie Van den HoeckeKatrin EhrhardtAnnasaheb KolpeKarim El BakkouriLei DengHendrik GrootaertSteve SchoonoogheAnouk SmetMostafa BentahirKenny Roose et al.

A1 Journal Article in JOURNAL OF VIROLOGY

Pretransplant glycomic analysis of perfusate is predictive of primary non function after liver transplantation

Xavier VerhelstAnja GeertsDieter VanderschaegheXavier RogiersAude VanlanderFrederik BerrevoetNico CallewaertRoberto TroisiHans Van Vlierberghe

Release of urinary extracellular vesicles in prostate cancer is associated with altered urinary N-glycosylation profile

Tijl VermassenKatharina D'HerdeDominique JacobusCharles Van PraetFilip PoelaertNicolaas LumenNico CallewaertKarel DecaesteckerGeert VilleirsPiet Hoebeke et al.

A1 Journal Article in JOURNAL OF CLINICAL PATHOLOGY

Screening proteomes for secretable fragments in yeast

Morgane BooneNico Callewaert

2016

A scalable low-cost cGMP process for clinical grade production of the HIV inhibitor 5P12-RANTES in Pichia pastoris

Fabrice CeriniHubert GaertnerKnut MaddenIlya TolstorukovScott BrownBram LaukensNico CallewaertJay C HarnerAnna M OommenJohn T Harms et al.

A1 Journal Article in PROTEIN EXPRESSION AND PURIFICATION

A sweet story of metabolic engineering : towards a yeast platform for production of triterpene saponins

Philipp ArendtAlain GoossensNico Callewaert

Elucidation of the molecular mechanisms of two nanobodies that inhibit thrombin‐activatable fibrinolysis inhibitor activation and activated thrombin‐activatable fibrinolysis inhibitor activity

X. ZhouS.D. WeeksPaul AmelootNico CallewaertS.V. StrelkovP.J. Declerck

A1 Journal Article in JOURNAL OF THROMBOSIS AND HAEMOSTASIS

Influenza-binding sialylated polymer coated gold nanoparticles prepared via RAFT polymerization and reductive amination

Zhiyue ZhangBert SchepensLutz NuhnXavier SaelensMichael SchotsaertNico CallewaertRiet De RyckeQilu ZhangSébastien MoinsSamira Benali et al.

A1 Journal Article in CHEMICAL COMMUNICATIONS

Long-lasting cross-protection against influenza A by neuraminidase and M2e-based immunization strategies

Michael SchotsaertTine YsenbaertAnouk SmetBert SchepensDieter VanderschaegheSvetlana StegalkinaThorsten U VogelNico CallewaertWalter FiersXavier Saelens

A1 Journal Article in SCIENTIFIC REPORTS

Modular integrated secretory system engineering in Pichia pastoris to enhance G-protein coupled receptor expression

Katrien ClaesKristof VandewalleBram LaukensToon LaeremansOlivier VostersIngrid LangerMarc ParmentierJan SteyaertNico Callewaert

A1 Journal Article in ACS SYNTHETIC BIOLOGY

N-glycosylation engineering in Pichia pastoris for protein therapeutics: advancing the GlycoSwitch and introducing GlycoDelete

Bram LaukensNico Callewaert

Synthetic biology for production of natural and new-to-nature terpenoids in photosynthetic organisms

Philipp ArendtJacob PollierNico CallewaertAlain Goossens

A1 Journal Article in PLANT JOURNAL

2015

Assesment of serum glycomics (GlycoCirrhoTest) for risk prediction of hepatocellular carcinoma development in patients with cirrhosis

Xavier VerhelstDieter VanderschaegheLaurent CasteraAnja GeertsNathalie GoutteClaire FrancozFrançois DurandNico CallewaertHans Van Vlierberghe

Assesment of serum glycomics (GlycoCirrhoTest) for risk prediction of hepatocellular carcinoma development in patients with cirrhosis

Xavier VerhelstDieter VanderschaegheLaurent CasteraAnja GeertsNathalie GoutteClaire FrancozFrançois DurandNico CallewaertHans Van Vlierberghe

Building genome-wide mutant resources in slow-growing mycobacteria

Kristof VandewalleNico CallewaertNele Festjens

Characterization of genome-wide ordered sequence-tagged Mycobacterium mutant libraries by Cartesian Pooling-Coordinate Sequencing

Kristof VandewalleNele FestjensEvelyn PletsMarnik VuylstekeYvan SaeysNico Callewaert

A1 Journal Article in NATURE COMMUNICATIONS

Comparison of VHH-Fc antibody production in Arabidopsis thaliana, Nicotiana benthamiana and Pichia pastoris

Thomas De MeyerBram LaukensJonah NolfEls Van LerbergeRiet De RyckeAns De BeuckelaerSylvie De BuckNico CallewaertAnna Depicker

A1 Journal Article in PLANT BIOTECHNOLOGY JOURNAL

Development of modular engineering tools for membrane protein expression in yeast

Katrien ClaesNico Callewaert

Efficacy of a trans-sialidase-ISCOMATRIX subunit vaccine candidate to protect against experimental Chagas disease

Iván Alejandro BontempiMiguel Hernán ViccoGabriel CabreraSilvina Raquel VillarFlorencia Belén GonzálezEduardo Angel RoggeroPaul AmelootNico CallewaertAna Rosa PérezIván Sergio Marcipar

A1 Journal Article in VACCINE

Engineering of protein production systems to drastically reduce glycan heterogeneity of biopharmaceuticals

Francis SantensNico Callewaert

Engineering of the production of mannose-6-phosphate-modified lysosomal enzymes by the yeast Pichia pastoris

Charlotte De VisscherNico Callewaert

Engineering the Pichia pastoris N-glycosylation pathway using the GlycoSwitch technology

Bram LaukensCharlot De WachterNico Callewaert

Bookchapter in Glyco-engineering : methods and protocols

Engineering yeast for producing human glycoproteins : where are we now?

Bram LaukensCharlotte De VisscherNico Callewaert

A1 Journal Article in FUTURE MICROBIOLOGY

Evaluation of the value of serum glycomics (GlycoCirrhoTest) for risk prediction of hepatocellular carcinoma in compensated cirrhosis

Xavier VerhelstDieter VanderschaegheLaurent CasteraAnja GeertsNathalie GoutteClaire FrancozFrançois DurandNico CallewaertHans Van Vlierberghe

Membrane protein expression and analysis in yeast

Katrien ClaesMouna GuerfalNico Callewaert

A1 Journal Article in METHODS IN ENZYMOLOGY

Production, purification and crystallization of a trans-sialidase from Trypanosoma vivax

Carole LF HaynesPaul AmelootHans RemautNico CallewaertYan G-J SterckxStefan Magez

A1 Journal Article in ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS

Structure and assembly mechanism of the signaling complex mediated by human CSF-1

Jan FélixSteven De MunckKenneth VerstraeteLeander MeurisNico CallewaertJonathan ElegheertSavvas Savvides

A1 Journal Article in STRUCTURE

Urinary prostate protein glycosylation profiling as a diagnostic biomarker for prostate cancer

Tijl VermassenCharles Van PraetNicolaas LumenKarel DecaesteckerDieter VanderschaegheNico CallewaertGeert VilleirsPiet HoebekeSimon Van BelleSylvie Rottey et al.

A1 Journal Article in PROSTATE

Using GlycoDelete to produce proteins lacking plant-specific N-glycan modification in seeds

Robin PironFrancis SantensAnnelies De PaepeAnna DepickerNico Callewaert

A1 Journal Article in NATURE BIOTECHNOLOGY

2014

Capillary electrophoresis of urinary prostate glycoproteins assists in the diagnosis of prostate cancer

Tijl VermassenCharles Van PraetDieter VanderschaegheThomas MaenhoutNicolaas LumenNico CallewaertPiet HoebekeSimon Van BelleSylvie RotteyJoris Delanghe

A1 Journal Article in ELECTROPHORESIS

Characterization of a type D1A EUL-related lectin from rice expressed in Pichia pastoris

Bassam Al AtalahDieter VanderschaegheYehudi BlochPaul ProostKirsten PlasNico CallewaertSavvas SavvidesEls Van Damme

A1 Journal Article in BIOLOGICAL CHEMISTRY

Genome dynamics of the human embryonic kidney 293 lineage in response to cell biology manipulations

Yao-Cheng LinMorgane BooneLeander MeurisIrma LemmensNadine Van RoyArne SoeteJoke ReumersMatthieu MoisseStéphane PlaisanceRadoje Drmanac et al.

A1 Journal Article in NATURE COMMUNICATIONS

GlycoDelete engineering of mammalian cells simplifies N-glycosylation of recombinant proteins

Leander MeurisFrancis SantensGreg ElsonNele FestjensMorgane BooneAnaëlle Dos SantosSimon DevosFrançois RousseauEvelyn PletsErica Houthuys et al.

A1 Journal Article in NATURE BIOTECHNOLOGY

Multiple phenotypes in phosphoglucomutase 1 deficiency

LC TegtmeyerS RustM van ScherpenzeelBG NgME LosfeldS TimalK RaymondP HeM IchikawaJ Veltman et al.

A1 Journal Article in NEW ENGLAND JOURNAL OF MEDICINE

Possible use of urinary prostate proteins glycosylation profile as a diagnostic biomarker for prostate cancer

Tijl VermassenNicolaas LumenCharles Van PraetDieter VanderschaegheNico CallewaertPiet HoebekeSimon Van BelleJoris DelangheSylvie Rottey

Vaccine production and glycoengineering in Arabidopsis thaliana seeds

Robin PironAnna DepickerNico Callewaert

2013

CE analysis of γ-globulin mobility and potential clinical utility

Dieter VanderschaegheEvi DebruyneHans Van VlierbergheNico CallewaertJoris Delanghe

Bookchapter in Clinical applications of capillary electrophoresis : methods and protocols

Enhanced membrane protein expression by engineering increased intracellular membrane production

Mouna GuerfalKatrien ClaesOskar KnittelfelderRiet De RyckeSepp D KohlweinNico Callewaert

A1 Journal Article in MICROBIAL CELL FACTORIES

High-throughput profiling of the serum N-glycome on capillary electrophoresis microfluidics systems

Dieter VanderschaegheAndras GuttmanNico Callewaert

Bookchapter in Clinical applications of capillary electrophoresis : methods and protocols

Necrostatin-1 blocks both RIPK1 and IDO: consequences for the study of cell death in experimental disease models

Peter VandenabeeleSasker GrootjansNico CallewaertNozomi Takahashi

Editorial material

Prostate proteins glycosylation profile and its potential as a diagnostic biomarker for prostate cancer

Tijl VermassenNicolaas LumenCharles Van PraetDieter VanderschaegheNico CallewaertPiet HoebekeSimon Van BelleSylvie RotteyJoris Delanghe

Structure of Ostertagia ostertagi ASP-1: insights into disulfide-mediated cyclization and dimerization

Jimmy BorlooPeter GeldhofIris PeelaersFrederik Van MeulderPaul AmelootNico CallewaertJozef VercruysseEdwin ClaereboutSergei V StrelkovStephen D Weeks

A1 Journal Article in ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

2012

A bacterial glycosidase enables mannose-6-phosphate modification and improved cellular uptake of yeast-produced recombinant human lysosomal enzymes

Petra TielsEkaterina BaranovaKathleen PiensCharlotte De VisscherGwenda PynaertWim NerinckxJan StoutFranck FudalejPaco HulpiauSimon Tännler et al.

A1 Journal Article in NATURE BIOTECHNOLOGY

A novel strategy for the comprehensive analysis of the biomolecular composition of isolated plasma membranes

Deepak B Thimiri Govinda RajBart GhesquièreArun Kumar Tharkeshwar RaghunathKatrijn CoenRita DeruaDieter VanderschaegheEvelien RysmanMurali BagadiPieter BaatsenBart De Strooper et al.

Biologically active, magnICON®-expressed EPO-Fc from stably transformed Nicotiana benthamiana plants presenting tetra-antennary N-glycan structures

Bieke NagelsEls Van DammeNico CallewaertLennart ZabeauJan TavernierJoris DelangheAnnemie BoetsAlexandra CastilhoKoen Weterings

A1 Journal Article in JOURNAL OF BIOTECHNOLOGY

Engineering the yeast Yarrowia lipolytica for the production of therapeutic proteins homogeneously glycosylated with Man₈GlcNAc₂ and Man₅GlcNAc₂

Karen De PourcqWouter VerveckenIsabelle DewerteAlbena ValevskaAnnelies Van HeckeNico Callewaert

A1 Journal Article in MICROBIAL CELL FACTORIES

Engineering Yarrowia lipolytica to produce glycoproteins homogeneously modified with the universal Man(3)GlcNAc(2) N-glycan core

Karen De PourcqPetra TielsAnnelies Van HeckeSteven GeysensWouter VerveckenNico Callewaert

A1 Journal Article in PLOS ONE

Introduction of tri-antennary N-glycans in Arabidopsis thaliana plants

Bieke NagelsEls Van DammeNico CallewaertKoen Weterings

A1 Journal Article in PLANT SCIENCE

N-glycan based biomarker distinguishing non-alcoholic steatohepatitis from steatosis independently of fibrosis

BRAM BLOMMESven FrancqueEric TrépoLouis LibbrechtDieter VanderschaegheAn VerrijkenPiet PattynYves Van NieuwenhoveDirk Van de PutteAnja Geerts et al.

A1 Journal Article in DIGESTIVE AND LIVER DISEASE

Necrostatin-1 analogues: critical issues on the specificity, activity and in vivo use in experimental disease models

Nozomi TakahashiLinde DuprezSasker GrootjansAnje CauwelsWim NerinckxJB DuHadawayVera GoossensRia RoelandtFilip Van HauwermeirenClaude Libert et al.

A1 Journal Article in CELL DEATH & DISEASE

Pichia surface display: a tool for screening single domain antibodies

Kristof De SchutterNico Callewaert

Bookchapter in Single domain antibodies : methods and protocols

Production of plant made pharmaceuticals: from plant host to functional protein

Bieke NagelsK WeteringsNico CallewaertEls Van Damme

A1 Journal Article in CRITICAL REVIEWS IN PLANT SCIENCES

Storage of blood samples at 4°C during one week does not affect protein glycosylation in healthy human volunteers

Xavier VerhelstBRAM BLOMMEWim DelvaNico CallewaertHans Van Vlierberghe

X-ray structure of Ostertagia ostertagi ASP-1 provides detailed insights in dimerization mechanism and protein cyclization

Jimmy BorlooPeter GeldhofIris PeelaersFrederik Van MeulderPaul AmelootNico CallewaertJozef VercruysseEdwin ClaereboutSergei StrelkovStephen D Weeks

2011

2D separation and profiling of complex oligosaccharide mixtures

Nathalie BoulyNico CallewaertAnita Van LandschootIngeborg Stals

A novel strategy for the comprehensive analysis of the biomolecular composition of isolated plasma membranes

Deepak B Thimiri Govinda RajBart GhesquièreArun Kumar Tharkeshwar RaghunathKatrijn CoenRita DeruaDieter VanderschaegheEvelien RysmanMurali BagadiPieter BaatsenBart De Strooper et al.

A1 Journal Article in MOLECULAR SYSTEMS BIOLOGY

Activation of a glycosyl hydrolase family 18 enzyme from the filamentous fungus Trichoderma reesei by C-terminal processing

Ingeborg StalsSaeid KarkehabadiPatricia NtarimaNico CallewaertMichael WardBart DevreeseMats Sandgren

Alterations of serum protein N-glycosylation in two mouse models of chronic liver disease are hepatocyte and not B cell driven

BRAM BLOMMEChristophe Van SteenkistePaola GrassiStuart M HaslamAnne DellNico CallewaertHans Van Vlierberghe

A1 Journal Article in AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY

Comparison of the hydrolytic capacity of Trichoderma reesei QM6a and RUT C30 enzyme mixtures on lignocellulosic substrates

Nathalie BoulyNico CallewaertIngeborg Stals

Disruption of the SapM locus in Mycobacterium bovis BCG improves its protective efficacy as a vaccine against M. tuberculosis

Nele FestjensPieter BogaertAnjana BatniErica HouthuysEvelyn PletsDieter VanderschaegheBram LaukensBob AsselberghEef ParthoensRiet De Rycke et al.

A1 Journal Article in EMBO MOLECULAR MEDICINE

Expression analysis of the nucleocytoplasmic lectin 'Orysata' from rice in Pichia pastoris

Bassam Al AtalahElke FouquaertDieter VanderschaeghePaul ProostJan BalzariniDavid F SmithPierre RougeYi LasanajakNico CallewaertEls Van Damme

A1 Journal Article in FEBS JOURNAL

Improved sample preparation for CE-LIF analysis of plant N-glycans

Bieke NagelsFrancis SantensKoen WeteringsEls Van DammeNico Callewaert

A1 Journal Article in ELECTROPHORESIS

Introduction of multi-antennary N-glycan structures in plants

Bieke NagelsEls Van DammeNico CallewaertKoen Weterings

Production of complex multiantennary N-Glycans in nicotiana benthamiana plants

Bieke NagelsEls Van DammeMartin PabstNico CallewaertKoen Weterings

A1 Journal Article in PLANT PHYSIOLOGY

Vaccine testing of recombinant versions of the protective activation-associated secreted proteins from the bovine abomasal parasite Ostertagia ostertagi

Peter GeldhofPaul AmelootNico CallewaertJ PootJozef VercruysseEdwin Claerebout

2010

2D separation and profiling of complex oligosaccharide mixtures

Nathalie BoulyNico CallewaertAnita Van LandschootIngeborg Stals

2D separation and profiling of complex oligosaccharide mixtures

Nathalie BoulyIngeborg StalsAnita Van LandschootNico Callewaert

Abscisic acid deficiency causes changes in cuticle permeability and pectin composition that influence tomato resistance to Botrytis cinerea

Katrien CurversHamed SeifiGrégory MouilleRiet De RyckeBob AsselberghAnnelies Van HeckeDieter VanderschaegheHerman R HöfteNico CallewaertFrank Van Breusegem et al.

A1 Journal Article in PLANT PHYSIOLOGY

Diagnostic Value of the Hemopexin N-Glycan Profile in Hepatocellular Carcinoma Patients

Evi DebruyneDieter VanderschaegheHans Van VlierbergheAnnelies VanheckeNico CallewaertJoris Delanghe

A1 Journal Article in CLINICAL CHEMISTRY

Engineering of glycosylation in yeast and other fungi: current state and perspectives

Karen De PourcqKristof De SchutterNico Callewaert

A1 Journal Article in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Expression, purification, biochemical characterisation and structure determination of a fungal endo-β-N-acetylglucosaminidase of glycosyl hydrolase family 18

Ingeborg StalsSaeid KarkehabadiMats SandgrenNico CallewaertAnita Van Landschoot

Fed-batch fermentation of GM-CSF-producing glycoengineered Pichia pastoris under controlled specific growth rate

Pieter JacobsM InanNele FestjensJurgen HaustraeteAnnelies Van HeckeRoland ContrerasMM MeagherNico Callewaert

A1 Journal Article in MICROBIAL CELL FACTORIES

GlycoHepatoTest: validation and analytical technology development for implementation on microfluidics analyzers

Dieter VanderschaegheJoris DelangheNico Callewaert

Glycome profiling using modern glycomics technology: technical aspects and applications

Dieter VanderschaegheNele FestjensJoris DelangheNico Callewaert

A1 Journal Article in BIOLOGICAL CHEMISTRY

High-throughput profiling of the serum N-glycome on capillary electrophoresis microfluidics systems: toward clinical implementation of glycoHepatoTest

Dieter VanderschaegheAkos SzekrenyesChristian WenzMarcus GassmannNatasha NaikMaggie BynumHongfeng YinJoris DelangheAndras GuttmanNico Callewaert

A1 Journal Article in ANALYTICAL CHEMISTRY

Isolation of antigen-binding camelid heavy chain antibody fragments (nanobodies) from an immune library displayed on the surface of Pichia pastoris

Stefan RyckaertE PardonJ SteyaertNico Callewaert

A1 Journal Article in JOURNAL OF BIOTECHNOLOGY

The HAC1 gene from Pichia pastoris: characterization and effect of its overexpression on the production of secreted, surface displayed and membrane proteins

Mouna GuerfalStefan RyckaertPieter JacobsPaul AmelootKathleen Van CraenenbroeckRiet De RyckeNico Callewaert

A1 Journal Article in MICROBIAL CELL FACTORIES

The impact of elevation of total bilirubin level and etiology of the liver disease on serum N-glycosylation patterns in mice and men

BRAM BLOMMEChristophe Van SteenkisteJacques VanhuysseIsabelle ColleNico CallewaertHans Van Vlierberghe

A1 Journal Article in AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY

The impact of elevation of total bilirubin level on serum N-glycosylation patterns in mice and men

BRAM BLOMMEChristophe Van SteenkisteJacques Van HuysseIsabelle ColleNico CallewaertHans Van Vlierberghe

The impact of elevation of total bilirubin level on serum N-glycosylation patterns in mice and men

BRAM BLOMMEChristophe Van SteenkisteJacques Van HuysseIsabelle ColleNico CallewaertHans Van Vlierberghe

2009

Alteration of protein glycosylation in liver diseases

BRAM BLOMMEChristophe Van SteenkisteNico CallewaertHans Van Vlierberghe

A1 Journal Article in JOURNAL OF HEPATOLOGY

Analysis of γ-globulin mobility on routine clinical CE equipment: exploring its molecular basis and potential clinical utility

Dieter VanderschaegheEvi DebruyneHans Van VlierbergheNico CallewaertJoris Delanghe

A1 Journal Article in ELECTROPHORESIS

Detection, separation and sequencing of oligosaccharides in beer

Ingeborg StalsKathleen PiensDieter DepuydtNico CallewaertBart DevreeseAnita Van Landschoot

Engineering complex-type N-glycosylation in Pichia pastoris using GlycoSwitch technology

Pieter P. JacobsSteven GeysensWouter VerveckenRoland ContrerasNico Callewaert

A1 Journal Article in NATURE PROTOCOLS

Genome sequence of the recombinant protein production host Pichia pastoris

Kristof De SchutterYao-Cheng LinPetra TielsAnnelies Van HeckeS GlinkaJ Weber-LehmannPierre RouzéYves Van de PeerNico Callewaert

A1 Journal Article in NATURE BIOTECHNOLOGY

GlycoFibroTest Is a Highly Performant Liver Fibrosis Biomarker Derived from DNA Sequencer-based Serum Protein Glycomics

Dieter VanderschaegheWouter LaroyErwin SablonPhilippe HalfonAnnelies Van HeckeJoris DelangheNico Callewaert

A1 Journal Article in MOLECULAR & CELLULAR PROTEOMICS

High-throughput quantitative analysis of plant N-glycan using a DNA sequencer

Jyung Jin LeeJin-Hee JungJung-Mi LeeYangkang SoOhsuk KwonNico CallewaertHyun Ah KangKisung KoDoo-Byoung Oh

A1 Journal Article in BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Hybrid N-glycans on the host protective activation-associated secreted proteins of Ostertagia ostertagi and their importance in immunogenicity (vol 161, pg 67, 2008)

Yves MeyvisNico CallewaertKris GevaertEvy TimmermanJoost Van DurmeJoost SchymkowitzFrederic RousseauJozef VercruysseEdwin ClaereboutPeter Geldhof

N-glycosylation Engineering of Biopharmaceutical Expression Systems

Pieter JacobsNico Callewaert

A1 Journal Article in CURRENT MOLECULAR MEDICINE

Open access to sequence: Browsing the Pichia pastoris genome

D MattanovichNico CallewaertPierre RouzéYC LinA GrafA RedlPetra TielsB GasserKristof De Schutter

A1 Journal Article in MICROBIAL CELL FACTORIES

Reversible changes in serum immunoglobulin galactosylation during the immune response and treatment of inflammatory autoimmune arthritis

Katrien Van BenedenKen CoppietersW LaroyFilip De KeyserIlse HoffmanFilip Van den BoschBert Vander CruyssenMichael DrennanPeggy JacquesP Rottiers et al.

A1 Journal Article in ANNALS OF THE RHEUMATIC DISEASES

2008

Consequences of soluble ICAM-1 N-glycan alterations on receptor binding and signaling kinetics in mouse astrocytes

Thomas SchürphNico CallewaertMatthias J.T. MeyerChristian TränkleWouter LaroyRichard D. CummingsVivianne Otto

A4 Journal Article in Open Glycoscience

Different N-glycosylation patterns in mice models of portal hypertension and cirrhosis

BRAM BLOMMEChristophe Van SteenkisteJacques VanhuysseIsabelle ColleNico CallewaertHans Van Vlierberghe

Fishing for lectins from diverse sequence libraries by yeast surface display - An exploratory study

Stefan RyckaertNico CallewaertPieter JacobsSylviane DewaeleIsabelle DewerteRoland Contreras

A1 Journal Article in GLYCOBIOLOGY

Glycosylation and cell surface engineering in Pichia pastoris

Pieter P JacobsNico CallewaertRoland Contreras

Hybrid N-glycans on the host protective activation-associated secreted proteins of Ostertagia ostertagi and their importance in immunogenicity

Yves MeyvisNico CallewaertKris GevaertEvy TimmermanJoost Van DurmeJoost SchymkowitzFrederic RousseauJozef VercruysseEdwin ClaereboutPeter Geldhof

A1 Journal Article in MOLECULAR AND BIOCHEMICAL PARASITOLOGY

Pichia surface display: display of proteins on the surface of glycoengineered Pichia pastoris strains

Pieter JacobsStefan RyckaertSteven GeysensKristof De VusserNico CallewaertRoland Contreras

A1 Journal Article in BIOTECHNOLOGY LETTERS

Synthesis of isofagomine and a new C-6 pyrrolidine azasugar with potential biological activity

Pieter EspeelKathleen PiensNico CallewaertJohan Van der Eycken

A1 Journal Article in SYNLETT

2007

Agalactosyl immunoglobulins are a useful marker for clinical progression and successful therapy of collagen-induced arthritis

Katrien Van BenedenKen CoppietersWouter LaroyPieter RottiersAugust VerbruggenNico CallewaertDirk Elewaut

An essential oligomannosidic glycan chain in the catalytic domain of autotaxin, a secreted lysophospholipase-D

S JANSENNico CallewaertIsabelle DewerteM ANDRIESH CEULEMANSM BOLLEN

A1 Journal Article in JOURNAL OF BIOLOGICAL CHEMISTRY

Clinical laboratory testing in human medicine based on the detection of glycoconjugates

BL SCHULZWouter LaroyNico Callewaert

A1 Journal Article in CURRENT MOLECULAR MEDICINE

Lyso-phosphatidylcholine is a signal in the arbuscular mycorrhizal symbiosis

D DRISSNERG KUNZENico CallewaertP GEHRIGM TAMASLOUKHTT BOLLERG FELIXN AMRHEINM BUCHER

A1 Journal Article in SCIENCE

Modification of the N-glycosylation pathway to produce homogeneous human-like glycans using GlycoSwitch plasmids

Wouter VerveckenNico CallewaertVladimir KaigorodovSteven GeysensRoland Contreras

Bookchapter in Pichia Protocols

N-glycomic changes in hepatocellular carcinoma patients with liver cirrhosis induced by hepatitis B virus

Xueen LiuLiesbeth DesmyterCF GAOWouter LaroySylviane DewaeleValerie VanhoorenL WANGH ZHUANGNico CallewaertClaude Libert et al.

A1 Journal Article in HEPATOLOGY

P-selectin mediates metastatic progression through binding to sulfatides on tumor cells

Josep GarciaNico CallewaertLubor Borsig

A1 Journal Article in GLYCOBIOLOGY

Parallel glycoprotein N-glycan analysis

Nico CallewaertSteven GeysensWouter Laroy

A1 Journal Article in GENETIC ENGINEERING & BIOTECHNOLOGY NEWS

2006

Angiogenesis enhances factor IX delivery and persistence from retrievable human bioengineered muscle implants

L THORREZH VANDENBURGHNico CallewaertNico MertensJ SHANSKYL WANGJ ARNOUTD COLLENM CHUAHT VANDENDRIESSCHE

A1 Journal Article in MOLECULAR THERAPY

Conserved oligomeric Golgi complex subunit 1 deficiency reveals a previously uncharacterized congenital disorder of glycosylation type II

F FOULQUIERE VASILEE SCHOLLENNico CallewaertT RAEMAEKERSD QUELHASJ JAEKENP MILLSB WINCHESTERM KRIEGER et al.

A1 Journal Article in PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Definition of the bacterial N-glycosylation site consensus sequence

Michael KowarikMartin N YoungShin NumaoBenjamin L SchulzIsabelle HugNico CallewaertDominic C MillsDavid C WatsonMarcela HernandezJohn F Kelly et al.

A1 Journal Article in EMBO JOURNAL

Glycome mapping on DNA sequencing equipment

Wouter LaroyRoland ContrerasNico Callewaert

A1 Journal Article in NATURE PROTOCOLS

N-glycan structures and N-glycosylation sites of mouse soluble intercellular adhesion molecule-1 revealed by MALDI-TOF and FTICR mass spectrometry

Vivianne I OttoEugen DamocLeah N CueniThomas SchurpfRenate FreiSarah AliNico CallewaertAdrian MoiseJulie A LearyGerd Folkers et al.

A1 Journal Article in GLYCOBIOLOGY

N-linked glycosylation of folded proteins by the bacterial oligosaccharyltransferase

Michael KowarikShin NumaoMario F FeldmanBenjamin L SchulzNico CallewaertEva KiermaierIna CatreinMarkus Aebi

A1 Journal Article in SCIENCE

Substrate specificity of bacterial oligosaccharyltransferase suggests a common transfer mechanism for the bacterial and eukaryotic systems

M WackerMF FeldmanNico CallewaertM KowarikBR ClarkeNL PohlM HernandezED VinesMA ValvanoC Whitfield et al.

A1 Journal Article in PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Two distinct but interchangeable mechanisms for flipping of lipid-linked oligosaccharides

C AlaimoI CatreinL MorfCL MaroldaNico CallewaertMA ValvanoMF FeldmanM Aebi

A1 Journal Article in EMBO JOURNAL

2005

Acceptor protein requirements for recombinant N-glycosylation in Escherichia coli

M KOWARIKI HUGNico CallewaertM HERNANDEZM WACKERM AEBI

Independent validation and comparison with fibroscan, fibrotest and liver biopsy of clinical glycomics for the non invasive assessment of liver fibrosis in chronic hepatitis C

L CASTERAAnnelies Van HeckeP TRIMOULETNico CallewaertP COUZIGOUV DE LEDINGHENRoland ContrerasWouter Laroy

N-glycan engineering in yeasts and fungi: finally human-like glycosylation

Nico CallewaertWouter VerveckenSteven GeysensRoland Contreras

Bookchapter in Handbook of Carbohydrate Engineering

O mannosylation of α-dystroglycan is essential for lymphocytic choriomeningitis virus receptor function

Mauro ImperialiClaudio ThomaErnesto PavoniAndrea BrancaccioNico CallewaertAnnette Oxenius

A1 Journal Article in JOURNAL OF VIROLOGY

2004

DSA-FACE: high-throughput analysis of the N-glycans of NS0-cell secreted antibodies

Lieselot DefrancqNico CallewaertJ ZHUWouter LaroyRoland Contreras

A2 Journal Article in Bioprocess International

In vivo synthesis of mammalian-like, hybrid-type N-glycans in Pichia pastoris

Wouter VerveckenVladimir KaigorodovNico CallewaertSteven GeysensKristof De VusserRoland Contreras

A1 Journal Article in APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Noninvasive diagnosis of liver cirrhosis using DNA sequencer-based total serum protein glycomics

Nico CallewaertHans Van VlierbergheAnnelies Van HeckeWouter LaroyJoris DelangheRoland Contreras

A1 Journal Article in NATURE MEDICINE

Total serum protein N-glycome profiling on a capillary electrophoresis-microfluidics platform

Nico CallewaertRoland ContrerasL MITNIK-GANKINL CAREYP MATSUDAIRAD EHRLICH

A1 Journal Article in ELECTROPHORESIS

2003

Increased fucosylation and reduced branching of serum glycoprotein N-glycans in all known subtypes of congenital disorder of glycosylation I

Nico CallewaertE SCHOLLENA VANHECKEJ JAEKENG MATTHIJSRoland Contreras

A1 Journal Article in GLYCOBIOLOGY

2002

Non-pathogenic trypanosomatid protozoa as a platform for protein research and production.

R BREITLINGS KLINGNERNico CallewaertR PIETRUCHAA GEYERG EHRLICHR HARTUNGA MULLERRoland ContrerasSM BEVERLEY et al.

A1 Journal Article in PROTEIN EXPRESSION AND PURIFICATION

Novel tools for the study of class I alpha-mannosidases: a chromogenic substrate and a substrate-analog inhibitor.

Tom DesmetWim NerinckxIngeborg StalsNico CallewaertRoland ContrerasMarc Claeyssens

A1 Journal Article in ANALYTICAL BIOCHEMISTRY

Structure and function in rhodopsin: High-level expression of rhodopsin with restricted and homogeneous N-glycosylation by a tetracycline-inducible N-acetylglucosaminyltransferase I-negative HEK293S stable mammalian cell line.

PJ REEVESNico CallewaertRoland ContrerasHG KHORANA

A1 Journal Article in PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Use of a meltable polyacrylamide matrix for sodium dodecyl sulfate-polyacrylamide gel electrophoresis in a procedure for N-glycan analysis on picomole amounts of glycoproteins.

Nico CallewaertWouter VerveckenA VAN HECKERoland Contreras

A1 Journal Article in ANALYTICAL BIOCHEMISTRY

2001

Ultrasensitive profiling and sequencing of N-linked oligosaccharides using standard DNA-sequencing equipment.

Nico CallewaertSteven GeysensP MOLEMANSRoland Contreras

A1 Journal Article in GLYCOBIOLOGY

Use of HDEL-tagged Trichoderma reesei mannosyl oligosaccharide 1,2-alpha-D-mannosidase for N-glycan engineering in Pichia pastoris.

Nico CallewaertWouter LaroyH CADIRGISteven GeysensXavier SaelensWilly Min JouRoland Contreras

A1 Journal Article in FEBS LETTERS

2000

Molecular cloning and enzymatic characterization of a Trichoderma reesei 1,2-alpha-D-mannosidase.

Marleen MarasNico CallewaertKathleen PiensMarc ClaeyssensWim MartinetS DEWAELEH CONTRERASI DEWERTEM PENTTILARoland Contreras

A1 Journal Article in JOURNAL OF BIOTECHNOLOGY