The UGent NGS Core Facility requests funding to expand its infrastructure through an interdependent equipment package that enables reaction miniaturization, which reduces reagent costs, and increases throughput. This extension addresses growing demand for cost-efficient, scalable library preparation workflows for low-input and emerging applications such as single-cell sequencing, spatial transcriptomics, and CRISPR-based cell therapy research. To ensure contamination control and compliance with good laboratory practice, the equipment must be deployed across both pre- and post-PCR environments. As a functionally inseparable unit, it is essential for implementing standardized, miniaturized library preparation workflows. This investment will make high-throughput sequencing more efficient, affordable, and widely accessible to UGent research groups across disciplines.