Project

Immuno-PCR for the detection of Clostridium botulinum toxins in animals, feed and milk

Duration
01 October 2008 → 30 September 2010
Funding
Federal funding: various
Research disciplines
  • Natural sciences
    • Genetics
    • Microbiology
    • Systems biology
  • Medical and health sciences
    • Immunology
    • Laboratory medicine
    • Microbiology
    • Molecular and cell biology
    • Immunology
    • Laboratory medicine
    • Immunology
    • Laboratory medicine
    • Microbiology
    • Molecular and cell biology
Keywords
immuno-PCR toxins clostridium botulinum
 
Project description

The objective of the project is the development of in vitro detection methods for the different toxins (A, B, C, D, E and F) of Clostridium botulinum by optimizing both a competitive immuno-PCR test (icqPCR) and a quantitative immuno-PCR (iqPCR) and by comparing one to another with regard to the obtained specificity and sensitivity of detection for each type of botulinum toxin as well as to compare them to the reference method for detection of botulinum toxins being the mice toxicity test.