The objective of the project is the development of in vitro detection methods for the different toxins (A, B, C, D, E and F) of Clostridium botulinum by optimizing both a competitive immuno-PCR test (icqPCR) and a quantitative immuno-PCR (iqPCR) and by comparing one to another with regard to the obtained specificity and sensitivity of detection for each type of botulinum toxin as well as to compare them to the reference method for detection of botulinum toxins being the mice toxicity test.