Design and characterization of non-viral carriers for siRNA delivery in human skin

01 January 2009 → 31 October 2011
Regional and community funding: Special Research Fund
Research disciplines
  • Medical and health sciences
    • Dermatology
    • Dermatology
    • Biomarker discovery and evaluation
    • Drug discovery and development
    • Medicinal products
    • Pharmaceutics
    • Pharmacognosy and phytochemistry
    • Pharmacology
    • Pharmacotherapy
    • Toxicology and toxinology
    • Other pharmaceutical sciences
    • Dermatology
  • Engineering and technology
    • Biomaterials engineering
    • Biological system engineering
    • Biomaterials engineering
    • Biomechanical engineering
    • Other (bio)medical engineering
    • Environmental engineering and biotechnology
    • Industrial biotechnology
    • Other biotechnology, bio-engineering and biosystem engineering
myosin Va siRNA ultradeformable liposomes
Project description

Ultradeformable liposomes and ethosomes are synthesized and complexed with siRNA agaisnt myosin Va exon F, an important transportation protein involved in pigmentation. Their size, surface charge and deformability are investigated. Human primary melanocytes are transfected with these complexes to evaluate their biological activity (i.e. their silencing capacity) cytotoxicity and stability in time. Finally their penetration capacity through human skin will be tesetd by means of Franz-diffusion cells and evaluation is done by multiphoton microscopy.