Code
01SM2113
Duration
01 October 2013 → 31 May 2014
Funding
Regional and community funding: Special Research Fund
Promotor
Fellow
Research disciplines
-
Natural sciences
- Analytical chemistry
- Other chemical sciences
-
Medical and health sciences
- Nutrition and dietetics
- Pharmaceutical analysis and quality assurance
-
Engineering and technology
- Biomaterials engineering
- Biological system engineering
- Biomaterials engineering
- Biomechanical engineering
- Other (bio)medical engineering
- Environmental engineering and biotechnology
- Industrial biotechnology
- Other biotechnology, bio-engineering and biosystem engineering
-
Agricultural and food sciences
- Agricultural animal production
- Food sciences and (bio)technology
Keywords
monoclonal antibody
hybridoma technique
phage display
amperometric immunosensor
deoxynivalenol
Project description
- the development of monoclonal antibody against deoxynivalenol using the classic hybridoma technique and the recombinant phage display technique. During recombinant antibody technologu the following steps will be used:
-> Construction of antobody library
-> Biopanning of the phage display library
-> Genetic engineering: point mutations
- The determination of deocynivalenol using an amperometric immunosesor based on carbon screen-printed electrodes.