Project

The development of a specific monoclonal antibody against deoxynvalenol

Duration
01 October 2013 → 31 May 2014
Funding
Regional and community funding: Special Research Fund
Research disciplines
  • Natural sciences
    • Analytical chemistry
    • Other chemical sciences
  • Medical and health sciences
    • Nutrition and dietetics
    • Pharmaceutical analysis and quality assurance
  • Engineering and technology
    • Biomaterials engineering
    • Biological system engineering
    • Biomaterials engineering
    • Biomechanical engineering
    • Other (bio)medical engineering
    • Environmental engineering and biotechnology
    • Industrial biotechnology
    • Other biotechnology, bio-engineering and biosystem engineering
  • Agricultural and food sciences
    • Agricultural animal production
    • Food sciences and (bio)technology
Keywords
monoclonal antibody hybridoma technique phage display amperometric immunosensor deoxynivalenol
 
Project description

- the development of monoclonal antibody against deoxynivalenol using the classic hybridoma technique and the recombinant phage display technique. During recombinant antibody technologu the following steps will be used:

-> Construction of antobody library

-> Biopanning of the phage display library

-> Genetic engineering: point mutations

- The determination of deocynivalenol using an amperometric immunosesor based on carbon screen-printed electrodes.