Project

Study of the phosphoenpyruvate-pyruvate-oxalacetate node in Eschericia coli based on multi-omics data and mathematical models

Code

01D26207

Duration

01 October 2007 → 30 September 2011

Funding

Regional and community funding: Special Research Fund

Promotor

Wim Soetaert

Research disciplines

Engineering and technology
- Biocatalysis and enzyme technology
- Bioprocessing, bioproduction and bioproducts
- Fermentation
- Industrial biotechnology diagnostics
- Industrial microbiology
- Industrial molecular engineering of nucleic acids and proteins
- Industrial biotechnology not elsewhere classified

Keywords

biotechnology modelling metabolic engineering transcriptomics metabolomics

Project description

This project has a goal to elucidate on of the most important nodes in industrial biotechnology, the PEP-pyruvate-oxalacetate node. By means of 13C-labellingsexperiments and transcriptome data, the intracellular fluxes will be uncovered. For this purpose, novel sampling and analytical protocols will be set up, after which experimental data can be collected as a basis for mathematical models.