The goal of this interdisciplinary research project is unraveling the role of Cu in liver disease and identification of the factors governing Cu isotope ratio variation by using mouse models and by further development of analytical methods. With these methods, we will have access to information on the isotopic composition of bulk Cu and of Cu in selected fractions and species, while we will also be able to reveal the spatial distribution of Cu and variation in 65Cu/63Cu down to a (sub-)cellular level.