A method for efficient CRISPR/Cas9 Gene editing in difficult to transform arthropods

17 January 2022 → 16 April 2023
Regional and community funding: Industrial Research Fund
Research disciplines
  • Natural sciences
    • Invertebrate biology
  • Engineering and technology
    • Genetically modified animals
  • Agricultural and food sciences
    • Agricultural plant protection
    • Biotechnology for agricultural, forestry, fisheries and allied sciences not elsewhere classified
insects endosomal escape reagent CRISPR/Cas9 mites GMO BAPC gene editing
Project description

In this concepTT, we want to capitalize on a unique gene editing method that we developed for the spider mite Tetranychus urticae. Mites - together with ticks, spiders and scorpions - belong to the Chelicerata, the second largest group of animals on the planet, and are notoriously difficult to genetically transform. We will collect data on variations in the formulation composition that might further improve editing efficiency. We will show that our method, in addition to spider mites, can be used in economically important predatory mites but also in some difficult to transform insects. Last but not least, we will provide proof of principle for the creation of knock-ins. Together this information will allow to broaden the claims of a planned patent.