Project

Targeting selected Transient Receptor Potential (TRP) channels to modulate gastric function in the weaned piglet

Code
3S016018
Duration
01 January 2018 → 31 December 2021
Funding
Research Foundation - Flanders (FWO)
Research disciplines
No data available
Keywords
weaned piglet
 
Project description

Weaning is a critical event in the life of the piglet because various stressors culminate. Due to its inability to adequately secrete acid and changes in motility, the function of the stomach is compromised and as a result the barrier function towards pathogenic bacteria and protein digestion is reduced. As a consequence, diarrhoea is frequently observed and farmers use massive amounts of antibiotics. Therefore, we want to modulate the function of the stomach by targeting TRPA1 and TRPV1 channels. These channels are present in various cell types in the stomach and small intestine, and it was shown in rodents that stimulation of these channels enhances secretions in the stomach and can delay emptying of gastric contents. The aim of my research is to explore the potential to modulate gastric function in the weaned piglet by using highly available phytochemicals that are known stimulators of these channels. First, the gene expression of TRPA1 and TRPV1 in different cell types along the gut will be investigated, and second an ex vivo model will be used to select the best phytochemicals for optimal modulation. Next, with these candidates, the modulatory effects of TRPA1 and TRPV1 stimulation will be examined in vivo using a cannulated pig model. Finally, an in vivo validation will be carried out to evaluate piglet performances and health. My research will lead to better formulations of feed for weaned piglets and lower use of antibiotics.