Code
01CD06524
Duration
01 November 2024 → 30 April 2025
Funding
Regional and community funding: Special Research Fund
Promotor
Fellow
Research disciplines
-
Natural sciences
- Carbohydrates
- Energy metabolism
- Regulation of metabolism
- Plant developmental and reproductive biology
- Interactomics
- Proteomics
-
Agricultural and food sciences
- Agricultural plant breeding and biotechnology
Keywords
Trehalose-6-Phosphate
Prime Editing
drought
sustainable agriculture
SPARK
stress resilience
yield
nitrogen
phenotypic screening assay
cell culture
Plant growth
source-sink
SNF1-related kinase 1
nutrient signalling
combinatorial CRISPR Cas9
gene modification
dynamic in vivo kinase reporter
precision breeding
Target of Rapamycin kinase
Project description
Source-sink communication is crucial for crop yield. TOR, SnRK1 and trehalose-6-P constitute the cornerstone of this nutrient signaling network regulating plant growth. Based on this network, highorder combinatorial CRISPR/Prime-edited mutant libraries in Arabidopsis will be screened for resilience and high-yield. Cell cultures as alternative will be evaluated. Besides, a dynamic kinase reporter will be developed to monitor TOR/SnRK1 activity in vivo.