Extracellular vesicles (EV), a heterogenous group of nanometer-sized structures released by all cells, are central to (patho)physiological processes, and hold great potential for clinical use. EV characterization by complementary techniques is essential to assess the quality of EV preparations obtained from biofluids. Nanoparticle tracking analysis (NTA) allows for a rapid and high-throughput characterization of EV preparations for size and concentration. To ensure continuation of the high-quality research activities, we request the replacement of the 13-year-old NTA instrument. We reached the end of the lifespan of the laser and spare parts are no longer available. As basic EV characterization, including EV abundance and size, is the basis for each EV-related experiment, the new NTA instrument is essential. Broad access will be ensured via the CORE Extracellular Vesicles.