Electrochemical study of the usability of gelatin, immobilized on electrode surfaces, as matrix for redox enzymes.

01 October 2009 → 13 January 2012
Regional and community funding: Special Research Fund, Research Foundation - Flanders (FWO)
Research disciplines
  • Natural sciences
    • Other biological sciences
    • Other natural sciences
Redox enzymes gelatin
Project description

  This project is part of the domain that is to gain insight into the mechanism and kinetics of processes taking place at modified electrodes. The latter have the potential to be used in the development of biosensors. In order corresponding target molecules to be detected quickly and with a high selectivity and sensitivity, the enzymatic activity of the enzymes must, which ISOLATED be immobilized on an electrode and bind the target molecules or react with them, to be guaranteed. The encapsulation of the enzymes in biocompatible matrices makes it one of the possible strategy & euml n. In this project the usability of gelatin for the immobilization of enzymes are investigated. In a first phase, different procedures will be compared to optimize the deposition of gelatin on various electrodes. The possibility of then linking of gelatin by means of a covalent bond to a gold electrode seems to be very promising. In a second phase, the incorporation of proteins or enzymes centrally in the deposited layer of gelatin. The model protein, horse heart cytochrome c will be used for this purpose in the first instance, after which it can be switched to a selected cytochrome P450 enzyme. Various conventional electrochemical techniques will be employed here. In addition, considerable attention will be paid to complete physico-chemical characterization of the deposited layer through various techniques.