A highly efficient CRISPR/Cas9 gene editing method for difficult to transform arthropods

01 September 2023 → 28 February 2025
European funding: framework programme
Research disciplines
  • Natural sciences
    • Genetics
  • Engineering and technology
    • Genetically modified animals
CRIPSR gene editing genetic transformation pest
Other information
Project description

During the ERC consolidator grant POLYADAPT, an efficient gene editing system was discovered which allows to genetically transform the spider mite, Tetranychus urticae, a main crop pest. This species belongs to the chelicerates, the second largest group of arthropods including mites, ticks and spiders, that are notoriously difficult to transform. Our invention not only accomplished for the first time gene knock-out in mites, but also allows precise gene editing via knock-in with high efficiency. The method results in a 20-fold increase of transformation efficiency. In the PoC CRISPART, we will demonstrate the efficacy of the invention on a wide range of difficult to transform arthropods and aim to better understand the mode of action. We will further investigate the optimal validation trajectory of the invention, considering a gene editing service and investigate commercial and financial feasibility of the optimal path.

Funded by the European Union. Views and opinions expressed are however those of the author(s) only and do not necessarily reflect those of the European Union or the European Research Council Executive Agency (ERCEA). Neither the European Union nor the authority can be held responsible for them.