Engineering of trehalose phosphorylase for the production of trehalose analogues

01 March 2014 → 30 September 2016
Regional and community funding: Special Research Fund
Research disciplines
  • Natural sciences
    • Biochemistry and metabolism
    • Systems biology
  • Medical and health sciences
    • Medical biochemistry and metabolism
    • Medical biochemistry and metabolism
    • Medical biochemistry and metabolism
  • Engineering and technology
    • Catalysis and reacting systems engineering
    • Chemical product design and formulation
    • General chemical and biochemical engineering
    • Process engineering
    • Separation and membrane technologies
    • Transport phenomena
    • Other (bio)chemical engineering
    • Biomaterials engineering
    • Biological system engineering
    • Biomaterials engineering
    • Biomechanical engineering
    • Other (bio)medical engineering
    • Environmental engineering and biotechnology
    • Industrial biotechnology
    • Other biotechnology, bio-engineering and biosystem engineering
trehalose mutagenesis biocatalysis
Project description

In this project, the substrate specificity of trehalose phosphorylase will be engineered by means of semi-rational mutagenesis. Both its donor and acceptor specificity will be modified towards alternative monosaccharides to allow the synthesis of various trehalose analogues. Such products have applications in the food industry as stabilizers with a mild sweet taste and low caloric content.