Evaluation of new tools to study the HIV latent reservoir in vitro and in vivo

01 January 2012 → 31 March 2017
Regional and community funding: Special Research Fund
Research disciplines
  • Medical and health sciences
    • Immunology
    • Immunology
    • Microbiology
    • Immunology
HIV next generation sequencing integration target capture laser capture microdissection
Project description

In this project new tools will be optimized to investigate latent HIV infection in vivo. Integration sites will be analyzed by optimizing a technique for sequence specific capture and DNA sequencing of proviral HIV DNA. In addition, a technique to identify latently infected cells based on in situ hybridization will be optimized for laser capture microdissection and gene expression analysis.