Cargo identification and dynamic organization of the plant-specific TPLATE endocytic complex and its interplay with AP2

01 January 2015 → 31 December 2020
Research Foundation - Flanders (FWO)
Research disciplines
  • Natural sciences
    • Regulation of metabolism
    • Intracellular compartments and transport
    • Plant genetics
clathrin-mediated endocytosis plant cell biology protein complex purification TPLATE complex
Project description

We recently identified the TPLATE complex as an essential endocytic complex driving plant clathrin-mediated endocytosis in plants alongside the evolutionary conserved AP-2 complex. Aim of this project is to link membrane proteins to either the TPLATE or the AP-2 complex and to further unravel the function of the TPLATE complex. This will be achieved using proximity biotinylation and conditional delocalization.