The importance of a balanced animal fiber pattern in the diet for gut and systemic health was demonstrated in cheetahs. The role of dietary animal fiber in the different terrestrial carnivore strategies and its link with health has however not been investigated. We aim at documenting the natural fiber intake of a broad range of carnivores (representing the variation of carnivorous strategies) and at pinning down its effects on the intestinal microbiome and gut health proxies. In a first stage, average prey intake and carcass-part selection behavior will be combined with analyzing different prey and prey parts for animal fiber concentrations in order to calculate animal fiber intake. Second, the microbiome that is associated with natural foraging behavior will be documented and put forward as gold standard. In a final step we will determine how deviations from a natural animal fiber intake pattern affect microbiome and gut health proxies (gut putrefaction and gut inflammation). This project will elucidate the role of animal fiber in the health in function of carnivore type which will enable animal fiber recommendations for the diet of carnivores under human care.