Project

Investigation of the spermfactor phospholipase C zeta associated with failed fertilization

Code

3F006309

Duration

01 October 2009 → 31 January 2013

Funding

Regional and community funding: Special Research Fund, Research Foundation - Flanders (FWO)

Promotor

Petra De Sutter

Fellow

Frauke Vanden Meerschaut

Research disciplines

Natural sciences
- Genetics
Medical and health sciences
- Endocrinology and metabolic diseases
- Gynaecology and obstetrics
- Laboratory medicine
- Medical systems biology
- Molecular and cell biology
- Morphological sciences
- Endocrinology and metabolic diseases
- Gynaecology and obstetrics
- Laboratory medicine
- Medical systems biology
- Molecular and cell biology
- Morphological sciences
- Nursing
- Endocrinology and metabolic diseases
- Gynaecology and obstetrics
- Laboratory medicine
- Medical systems biology
- Molecular and cell biology
- Morphological sciences

Keywords

knock-out mouse model failed fertilization oocyte activation

Project description

Failed fertilisation following intracytoplasmic sperm injection is mainly due to an oocyte activation defect. The sperm-specific phospholipase C zeta is the responsible factor. Its localisation, expression and genetics will be studied in activation deficient sperm. Furthermore we will create a knock-out mouse model for this factor, test different artificial oocyte stimulation ways and subsequently use these in human oocytes.