Project

Investigation of the spermfactor phospholipase C zeta associated with failed fertilization

Code
3F006309
Duration
01 October 2009 → 31 January 2013
Funding
Regional and community funding: Special Research Fund, Research Foundation - Flanders (FWO)
Research disciplines
  • Natural sciences
    • Genetics
  • Medical and health sciences
    • Endocrinology and metabolic diseases
    • Gynaecology and obstetrics
    • Laboratory medicine
    • Medical systems biology
    • Molecular and cell biology
    • Morphological sciences
    • Endocrinology and metabolic diseases
    • Gynaecology and obstetrics
    • Laboratory medicine
    • Medical systems biology
    • Molecular and cell biology
    • Morphological sciences
    • Nursing
    • Endocrinology and metabolic diseases
    • Gynaecology and obstetrics
    • Laboratory medicine
    • Medical systems biology
    • Molecular and cell biology
    • Morphological sciences
Keywords
knock-out mouse model failed fertilization oocyte activation
 
Project description

Failed fertilisation following intracytoplasmic sperm injection is mainly due to an oocyte activation defect. The sperm-specific phospholipase C zeta is the responsible factor. Its localisation, expression and genetics will be studied in activation deficient sperm. Furthermore we will create a knock-out mouse model for this factor, test different artificial oocyte stimulation ways and subsequently use these in human oocytes.