Wounds on the distal limbs of horses pose a significant risk of complicated healing. An often-observed complication is the excessive growth of granulation tissue, known as exuberant granulation tissue (EGT), which extends well beyond the wound margins and impedes wound closure. This wound healing disorder presents a significant challenge to equine veterinary care. EGT is a leading cause of early retirement and can even result in the death or euthanasia of horses. Currently, there is no effective treatment, largely due to a lack of understanding of the underlying mechanisms. In the clinic, some horses with EGT recover quickly, and these are not considered a major problem, but some horses show chronic EGT. These require extended treatment and are most at risk for severe complications. This project aims to enhance our comprehension of the immune landscape and its impact on fibroblasts by comparing fast and slow healing EGT. The methodology will involve basic histological analysis, laser capture microdissection, and sequencing. In addition, in vitro stimulation experiments will be conducted to investigate the effect of cytokines and immune cells on fibroblasts. Finally, we will investigate the wound microbiome as EGT wounds are never sterile and the microbiome's composition can directly affect EGT. These insights will be crucial to identify more effective treatment strategies for horse limb wounds.