In the context of skeletal muscle tissue engineering, conductive hydrogel materials have proven successful in promoting the myogenesis process but come with the drawbacks of non-biodegradability, water insolubility, poor mechanical properties and debated in vivo cytotoxicity. A resort to ionic conductivity will be made instead which relies on ion transport, like all biological processes, by developing an ion conducting gelatin hydrogel which will address the above-mentioned shortcomings. Enhancing the ionic conductivity will be achieved by grafting photo-crosslinkable ionic polymers, synthesized using RAFT, onto a gelatin backbone. The systematic investigation of the factors influencing the ionic conductivity by altering the chemical design of ionic polymers is unprecedented in the field. The functionalized and characterized materials will be processed into aligned scaffolds by means of extrusion-based 3D-bioprinting in a support bath in the presence of C2C12 myoblasts. An electric field parallel to the printing nozzle will be applied which is hypothesized to enhance C2C12 myoblast alignment. Assessing the effect of in vitro electric field stimulation on the myogenesis process will be realized by measuring the calcium signaling, tetanic force, expression of myogenic regulatory factors, nuclear aspect ratio and myoblast alignment for a cationic, anionic and neutral ion conducting hydrogel.