Dendritic cells, the professional antigen-presenting cells of our immune system, induce cytotoxic T lymphocyte responses against tumor-associated antigens. Consequently, there has been much interest in their application as anti-tumor vaccines. In this study we willcombine the expertise of the GRGN and LMCT to evaluate the use of mRNA-loaded microbubbles and ultrasound to modify DCs ex vivo as well as intradally to boost anti-tumor immunity. These data together with the fact that the use of mRNA-loaded microbubbles could provide a way to manipulate DCs in vio, warrant an in dept evaluation of this mRNA-loaded microbubble-based vaccination strategy.