Research Explorer

Project

Improving multiplex digital PCR based nucleic acid quantification using data driven analysis tools
Code
01IO0420
Duration
01 October 2020 → 30 September 2024
Funding
Regional and community funding: Special Research Fund
Research disciplines
  • Natural sciences
    • Statistics not elsewhere classified
  • Medical and health sciences
    • Molecular diagnostics
    • Cancer diagnosis
    • Biostatistics
  • Agricultural and food sciences
    • Veterinary medicine not elsewhere classified
Keywords
Digital Polymerase Chain Reaction (dPCR) multiplex PCR macrophages single cell gene expression cancer mutation detection
 
Project description
 

Digital PCR allows higher order multiplexing compared to real time PCR, enabling simultaneous quantification of multiple targets for single cell research and for diagnosis of cancer mutations or infectious agents. These applications require an appropriate data analysis pipeline. We will tackle this unexplored field by designing 16-plex reactions for single cell analysis of macrophages and mutation detection in cancer.