Improving multiplex digital PCR based nucleic acid quantification using data driven analysis tools

01 October 2020 → 30 September 2024
Regional and community funding: Special Research Fund
Research disciplines
  • Natural sciences
    • Statistics
  • Medical and health sciences
    • Molecular diagnostics
    • Cancer diagnosis
    • Biostatistics
  • Agricultural and food sciences
    • Veterinary medicine not elsewhere classified
Digital Polymerase Chain Reaction (dPCR) multiplex PCR macrophages single cell gene expression cancer mutation detection
Project description

Digital PCR allows higher order multiplexing compared to real time PCR, enabling simultaneous quantification of multiple targets for single cell research and for diagnosis of cancer mutations or infectious agents. These applications require an appropriate data analysis pipeline. We will tackle this unexplored field by designing 16-plex reactions for single cell analysis of macrophages and mutation detection in cancer.