Project

Optimalisation of in vitro culture conditions (FTOC and OP9) of T cells from human hematopoietic stem cells for the generation and study of mature T cells

Duration
01 January 2008 → 31 December 2010
Funding
Research Foundation - Flanders (FWO)
Promotor
Research disciplines
  • Medical and health sciences
    • Immunology
    • Laboratory medicine
    • Palliative care and end-of-life care
    • Regenerative medicine
    • Other basic sciences
    • Immunology
    • Laboratory medicine
    • Palliative care and end-of-life care
    • Regenerative medicine
    • Other clinical sciences
    • Other health sciences
    • Nursing
    • Other paramedical sciences
    • Immunology
    • Laboratory medicine
    • Palliative care and end-of-life care
    • Regenerative medicine
    • Other translational sciences
    • Other medical and health sciences
Keywords
tumor antigen immunotherapy T cell
 
Project description

In this project we will further study and optimise the generation of mature CD3+TCRab+ (CD4+ and CD8+) T-cells from human HSC (from postnatal thymus and cord blood) using the in vitro OP9-DL1 culture system. Several methods for the generation of tumor antigen specific T-cells will be tested out. Finally, these tumor antigen specific T-cells will be introduced in a preclinical mouse model.