Plant growth and development depend on a tight regulation of water and small solute transport across cell membranes and tissues. Water and solute diffusion through membranes is facilitated by a class of integral plasma membrane proteins called aquaporins. These proteins also play a major role in gas exchange in leaves as they control the opening and closure of the stomatal pores. An interactomics experiment linked several aquaporins to the Secretory Carrier-Associated Membrane Protein 5 (SCAMP5). SCAMPs are highly conserved small integral membrane proteins involved in endomembrane trafficking. Plant SCAMPs are very poorly characterized and although they have been linked to secretion of cell wall components and to stomatal dynamics, we know very little about their role. The main goal of this project is to unravel how SCAMP proteins interact with, and control the function of the aquaporins. This will be done by mapping the SCAMP family interactome and by establishing direct interactions between SCAMPs and aquaporins. Furthermore, we will identify key residues required for their interaction using Alphafold structural modelling in combination with molecular dynamics simulations. We will characterize the role of their interaction in planta and in a Xenopus oocyte model using SCAMPs and aquaporins carrying specific mutations that abolish their interaction. Finally, we will visualize SCAMP and aquaporin trafficking and determine their interplay in stomata upon abiotic stress.